A bioinspired gelatin-hyaluronic acid-based hybrid interpenetrating network for the enhancement of retinal ganglion cells replacement therapy

Dromel, Pierre C.; Singh, Deepti; Andres, Eliot; Likes, Molly; Kurisawa, Motoichi; Alexander‐Katz, Alfredo; Spector, Myron; Young, Michael J.

doi:10.1038/s41536-021-00195-3

Cited by 18 publications

(14 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The engrafted hRGCs display signs of optic nerve regeneration via decreased immune cell response to hRGCs delivered in the gel. 40 Hydrogel properties also influence release properties along with cell behavior. On a separate study from the same group, the diffusion and release of human epidermal growth factors (hEGF)…”

Section: Retinamentioning

confidence: 99%

“…A study developed IPNs of gelatin‐hydroxyphenyl propionic acid (Gtn‐HPA) and HA‐tyramine (HA‐Tyr) hydrogels and tuned them to mimic the vitreous chemical composition and mechanical properties. These vitreous mimics improve in vitro and in vivo viability of human retinal ganglion cells (hRGCs) in the IPNs 40 . In vivo vitreal injections of cell laden IPNs show attachment of cells to the inner limiting membrane of rat retinas to improve with hydrogel stiffness.…”

Section: Ophthalmologymentioning

confidence: 99%

See 1 more Smart Citation

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

Adhikari

Stager

Krebs

2023

J Biomedical Materials Res

View full text Add to dashboard Cite

The field of biomaterials aims to improve regenerative outcomes or scientific understanding for a wide range of tissue types and ailments. Biomaterials can be fabricated from natural or synthetic sources and display a plethora of mechanical, electrical, and geometrical properties dependent on their desired application. To date, most biomaterial systems designed for eventual translation to the clinic rely on soluble signaling moieties, such as growth factors, to elicit a specific cellular response. However, these soluble factors are often limited by high cost, convoluted synthesis, low stability, and difficulty in regulation, making the translation of these biomaterials systems to clinical or commercial applications a long and arduous process. In response to this, significant effort has been dedicated to researching cell‐directive biomaterials which can signal for specific cell behavior in the absence of soluble factors. Cells of all tissue types have been shown to be innately in tune with their microenvironment, which is a biological phenomenon that can be exploited by researchers to design materials that direct cell behavior based on their intrinsic characteristics. This review will focus on recent developments in biomaterials that direct cell behavior using biomaterial properties such as charge, peptide presentation, and micro‐ or nano‐geometry. These next generation biomaterials could offer significant strides in the development of clinically relevant medical devices which improve our understanding of the cellular microenvironment and enhance patient care in a variety of ailments.

show abstract

Section: Retinamentioning

confidence: 99%

Section: Ophthalmologymentioning

confidence: 99%

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

Adhikari

Stager

Krebs

2023

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…In this respect, double crosslinked HA networks were synthesized by Diels-Alder click reaction combined with phenyl boronate ester bonds, resulting in superior mechanical properties, good injectability and adhesion, and reduced degradation [241]. Bioinspired hydrogel designed as IPN of gelatin-hydroxyphenyl propionic acid and HA-tyramine were proposed for enhancing intravitreal retinal cell therapy [242]. Horseradish peroxidase and hydrogen peroxide incorporated into the hydrogel enabled the control of gelation rate and crosslinking density.…”

Section: Hyaluronic Acidmentioning

confidence: 99%

Bioinspired Hydrogels as Platforms for Life-Science Applications: Challenges and Opportunities

Bercea¹

2022

Polymers

View full text Add to dashboard Cite

Hydrogels, as interconnected networks (polymer mesh; physically, chemically, or dynamic crosslinked networks) incorporating a high amount of water, present structural characteristics similar to soft natural tissue. They enable the diffusion of different molecules (ions, drugs, and grow factors) and have the ability to take over the action of external factors. Their nature provides a wide variety of raw materials and inspiration for functional soft matter obtained by complex mechanisms and hierarchical self-assembly. Over the last decade, many studies focused on developing innovative and high-performance materials, with new or improved functions, by mimicking biological structures at different length scales. Hydrogels with natural or synthetic origin can be engineered as bulk materials, micro- or nanoparticles, patches, membranes, supramolecular pathways, bio-inks, etc. The specific features of hydrogels make them suitable for a wide variety of applications, including tissue engineering scaffolds (repair/regeneration), wound healing, drug delivery carriers, bio-inks, soft robotics, sensors, actuators, catalysis, food safety, and hygiene products. This review is focused on recent advances in the field of bioinspired hydrogels that can serve as platforms for life-science applications. A brief outlook on the actual trends and future directions is also presented.

show abstract

“…In brief, the ONL was defined as the distance between the outer plexiform layer to the outer limiting membrane, and was assessed by the averaged thicknesses from 10 locations on each image, measured at 700 μm intervals by 5 locations on each side of the optic disc. The measurements of ONL thickness were performed by experienced investigators in a masked fashion [58][59][60].…”

Section: Optical Coherence Tomography (Oct)mentioning

confidence: 99%

IL-4 induces reparative phenotype of RPE cells and protects against retinal neurodegeneration via Nrf2 activation

Zhou

Yang

et al. 2022

Cell Death Dis

View full text Add to dashboard Cite

Retinal degeneration is a kind of neurodegeneration characterized by progressive neuronal death and dysfunction of retinal pigment epithelium (RPE) cells, leading to permanent visual impairment. It still lacks effective therapeutic options and new drugs are highly warranted. In this study, we found the expression of IL-4, a critical regulator of immunity, was reduced in both patients and mouse models. Importantly, exogenous intravitreal IL-4 application could exert a novel neuroprotective effect, characterized by well-preserved RPE layer and neuroretinal structure, as well as amplified wave-amplitudes in ERG. The RNA-seq analysis revealed that IL-4 treatment suppressed the essential oxidative and pro-inflammatory pathways in the degenerative retina. Particularly, IL-4 upregulated the IL-4Rα on RPE cells and induced a reparative phenotype via the activation of Nrf2 both in vitro and in vivo. Furthermore, the Nrf2-/- mice displayed no recovery in response to IL-4 application, highlighting a significant role of Nrf2 in IL-4-mediated protection. Our data provides evidence that IL-4 protects against retinal neurodegeneration by its antioxidant and anti-inflammatory property through IL-4Rα upregulation and Nrf2 activation in RPE cells. The IL-4/IL-4Rα-Nrf2 axis maybe the potential targets for the development of novel therapies for neurodegenerative diseases.

show abstract

A bioinspired gelatin-hyaluronic acid-based hybrid interpenetrating network for the enhancement of retinal ganglion cells replacement therapy

Cited by 18 publications

References 57 publications

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

Cell‐instructive biomaterials in tissue engineering and regenerative medicine

Bioinspired Hydrogels as Platforms for Life-Science Applications: Challenges and Opportunities

IL-4 induces reparative phenotype of RPE cells and protects against retinal neurodegeneration via Nrf2 activation

Contact Info

Product

Resources

About