Controlling adsorption of albumin with hyaluronan on silica surfaces and sulfonated latex particles

Berts, Ida; Fragneto, Giovanna; Porcar, Lionel; Hellsing, Maja S.; Rennie, Adrian R.

doi:10.1016/j.jcis.2017.05.037

Cited by 4 publications

(3 citation statements)

References 58 publications

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“…It is well established that the surfaces of nanoparticles are covered by various kinds of proteins and other biological molecules when in contact with a biological system. − As a result, the nanoparticles now exist as a new entity, a complex of nanoparticles with layers of proteins (so-called protein corona ,, ), whose surface characteristics alter depending on its composition. The nature of protein coronae is also dependent on the intrinsic properties of the nanoparticles, their chemical compositions, , shape, and surface chemistry. ,, Proteins within coronae are divided into two groups: “hard” and “soft” coronae.…”

Section: Introductionmentioning

confidence: 99%

Soft and Hard Interactions between Polystyrene Nanoplastics and Human Serum Albumin Protein Corona

et al. 2019

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Upon contact with biological fluids, the surface of nanoparticles is surrounded by many types of proteins, forming a so-called "protein corona". The physicochemical properties of the nanoparticle/corona complex depend predominantly on the nature of the protein corona. An understanding of the structure of the corona and the resulting complex provides insight into the structure−activity relationship. Here, we structurally evaluate the soft and hard components of the protein corona, formed from polystyrene (PS) nanoplastics and human serum albumin (HSA). Using circular dichroism spectroscopy to elucidate the structure of HSA within the complex, we establish the effect of nanoparticle size and pH on the nature of the protein corona formed-whether hard or soft. Despite the weak interaction between PS and the HSA corona, small angle neutron scattering revealed the formation of a complex structure that enhanced the intermolecular interactions between HSA proteins, PS particles, and the HS/PSA complexes. Fractal formation occurred under conditions where the interaction between PS and HSA was strong, and increasing HSA concentrations suppressed the degree of aggregation. The size of the nanoparticles directly influenced the nature of the protein corona, with larger particles favoring the formation of a soft corona, due to the decreased PS−HSA attraction.

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Section: Introductionmentioning

confidence: 99%

Soft and Hard Interactions between Polystyrene Nanoplastics and Human Serum Albumin Protein Corona

et al. 2019

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“…Changes in epithelial cell–cell adhesion have been shown to occur through changes in cell–substrate adhesion, which is controlled by interaction with ECM and other surface proteins. − We observe significant alteration to ECM protein adsorption on SiO 2 substrates derived from ⟨100⟩ Si wafers when compared to the other SiO 2 materials, and this may be the basis for the distinct cell behaviors that we observe. Protein interactions with silica materials have been shown to involve both polar and nonpolar interactions and are controlled by solvent conditions such as pH and the presence of soluble biomaterials such as hyaluronic acid as well as protein size and morphology. − Although the SiO 2 substrates have similar mechanical and compositional properties, they do have very subtle topographical differences that may contribute to some of the changes in cell behavior. Nevertheless, we showed that the surface energy of these materials is significantly different between the SiO 2 substrate derived from ⟨100⟩ and the other SiO 2 substrates.…”

Section: Discussionmentioning

confidence: 99%

Alternative SiO₂ Surface Direct MDCK Epithelial Behavior

Covell

Zeng

Mabe

et al. 2017

ACS Biomater. Sci. Eng.

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The mechanical interactions of cells are mediated through adhesive interactions. In this study, we examined the growth, cellular behavior, and adhesion of MDCK epithelial cells on three different SiO2 substrates: amorphous glass coverslips and the silicon oxide layers that grow on ⟨111⟩ and ⟨100⟩ wafers. While compositionally all three substrates are almost similar, differences in surface energy result in dramatic differences in epithelial cell morphology, cell–cell adhesion, cell–substrate adhesion, actin organization, and extracellular matrix (ECM) protein expression. We also observe striking differences in ECM protein binding to the various substrates due to the hydrogen bond interactions. Our results demonstrate that MDCK cells have a robust response to differences in substrates that is not obviated by nanotopography or surface composition and that a cell’s response may manifest through subtle differences in surface energies of the materials. This work strongly suggests that other properties of a material other than composition and topology should be considered when interpreting and controlling interactions of cells with a substrate, whether it is synthetic or natural.

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“…Albumin has previously been shown to interact weakly with HA-coated silica surfaces at neutral pH. 50 Apo-A1 and serum albumin are common constituents of the protein corona of various nanoparticles sub-sequent to plasma exposition. 51 Clearly stronger band intensities in the same molecular weight regions, and additionally in the molecular weight range >88 kDa, were seen for both MSN-COOH and MSN-PEG particles, and the band intensities were very similar for both sets of particles.…”

Section: Papermentioning

confidence: 99%

Towards a simple in vitro surface chemistry pre-screening method for nanoparticles to be used for drug delivery to solid tumours

Schmid

Kaiser²,

Willbold³

et al. 2023

Biomater. Sci.

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Controlling adsorption of albumin with hyaluronan on silica surfaces and sulfonated latex particles

Cited by 4 publications

References 58 publications

Soft and Hard Interactions between Polystyrene Nanoplastics and Human Serum Albumin Protein Corona

Soft and Hard Interactions between Polystyrene Nanoplastics and Human Serum Albumin Protein Corona

Alternative SiO₂ Surface Direct MDCK Epithelial Behavior

Towards a simple in vitro surface chemistry pre-screening method for nanoparticles to be used for drug delivery to solid tumours

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Controlling adsorption of albumin with hyaluronan on silica surfaces and sulfonated latex particles

Cited by 4 publications

References 58 publications

Soft and Hard Interactions between Polystyrene Nanoplastics and Human Serum Albumin Protein Corona

Soft and Hard Interactions between Polystyrene Nanoplastics and Human Serum Albumin Protein Corona

Alternative SiO2 Surface Direct MDCK Epithelial Behavior

Towards a simple in vitro surface chemistry pre-screening method for nanoparticles to be used for drug delivery to solid tumours

Contact Info

Product

Resources

About

Alternative SiO₂ Surface Direct MDCK Epithelial Behavior