Controlled synthesis of mesoporous nanostructured anatase TiO₂on a genetically modified Escherichia coli surface for high reversible capacity and long-life lithium-ion batteries

Abstract

TiO 2 is a promising anode material for lithium-ion batteries. The electrochemical performance of TiO 2 can be improved by optimization of nanostructures. The present study was proposed to control the synthesis of mesoporous nanostructured anatase TiO 2 on a genetically modified Escherichia coli surface. A recombinant protein INP-SiliSila containing functional domains of silicatein-a and silaffin was constructed and expressed on the E. coli surface. Deposition of the TiO 2 precursor was facilitated by INP-SiliSila on the E. coli surface. Upon calcination, TiO 2 coating on the E. coli surface transformed to anatase and formed well-defined rod-shaped particles. The electrochemical performance of the asprepared anatase TiO 2 as anode electrodes was improved and better than that of most reported ones.The present study not only provides an organism-based approach for fabricating nanostructured anatase TiO 2 with enhanced electrochemical performance, but also opens a new avenue to take advantage of genetically modified bacterial surfaces in the synthesis and structure control of materials.

Microbe‐Mediated Biosynthesis of Multidimensional Carbon‐Based Materials for Energy Storage Applications

“…After carbonization, the volume of the cells obviously shrunk to 0.5 µm as shown in Figure 3b,c. To further avoid the contamination of bacteria, some specific antibiotic, such as ampicillin and kanamycin, should be added into the LB medium, which can allow the selected bioengineered E. coli bacteria to grow on the culture medium while preventing the growth of other nonresistant bacteria, [95,96] thus promoting the formation of unified E. coli colony in the fermentation process. In the production process, E. coli bacteria with only a large circular DNA molecule were vegetative propagated by dichotomous division.…”

“…Then, Su's group [95] further expressed two functional domains of silaffin and silicatein-a on the surface of E. coli bacteria cells via genetic manipulation technology based on the stable carrier host of ice nucleation protein expressed by adding gene segment of ice nucleation protein (inaK)35 (accession number AF013159) extracted from Pseudomonas syringae), named as INP-SiliSila. Silicatein was expressed via modifying gene segment of silicatein-a15 (accession number AJ272013) derived from the sponge Suberites domuncula while the silaffin was based on the gene segment of silaffin precursor protein sil1p (sil1)29 (accession number AF191634.1) offered by the sponge Cylindrotheca fusiformis.…”

“…To further enhance the deposition of TiO 2 materials on modified surface of E. coli bacteria, Xue et al [95] further expressed two functional domains of silaffin and silicatein-a on the surface of E. coli bacteria cells via modifying gene segment of silicatein-a15 and silaffin precursor protein sil1p (sil1)29. The incorporation of the silaffin and silicatein-a can effectively improve the biocatalytic synthesis of nanosized materials on the modified surface of E. coli bacteria.…”

Low cost Na2FeSiO4/H–N-doped hard carbon nanosphere hybrid cathodes for high energy and power sodium-ion supercapacitors

“…Low cost lithium ion batteries (LIBs) with high energy/power densities, high rate performance and long cycling stability have attracted enormous attention due to good safety and its potential applications in future electric vehicles, smart electric grids, and even miniaturized electronic/optoelectronic devices, etc [1][2][3][4]. In design and fabrication of low-cost sodium ion batteries (SIBs), the rational design of promising cathode is highly challenging yet critically important in practical applications.…”

The effects of structural properties on the lithium storage behavior of mesoporous TiO2

“…A popular and effective way to address these issues is to utilize mesoporous TiO 2 with micron or submicron particle sizes instead of monodispersed nanoparticles [13,[19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36]. For instance, a superior reversible capacity of 220 mA h g −1 at 1C has been reported by Saravanan et al for the micronsized mesoporous TiO 2 with high packing density [19].…”