Controlled Semi-Automated Laser-Induced Injuries for Studying Spinal Cord Regeneration in Zebrafish Larvae

El-Daher, Francois; Early, Jason J; Richmond, Claire; Jamieson, Rory; Becker, Catherina G.; Becker, Thomas

doi:10.3791/63259

Cited by 1 publication

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“…These methods have increased screening efficiency to the point that several hundred genes can be functionally tested by a single researcher in a matter of weeks. The optical accessibility and lack of skeletal tissues in larval zebrafish also allow the use of a range of rapid injury paradigms from simple physical transections to semi-automated laser injuries and cell-specific photoablation (Briona and Dorsky, 2014b ; Hecker et al, 2020 ; El-Daher et al, 2021 ). Further, a diverse catalog of quantifiable locomotor behaviors including swim kinematics, swim endurance, and escape response, facilitates simple and robust assessment of subsequent functional recovery (Mokalled et al, 2016 ; Hecker et al, 2020 ; Vasudevan et al, 2021 ).…”

Section: Advantages Of the Larval Zebrafish Modelmentioning

confidence: 99%

Unique advantages of zebrafish larvae as a model for spinal cord regeneration

Alper

Dorsky

2022

Front. Mol. Neurosci.

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The regenerative capacity of the spinal cord in mammals ends at birth. In contrast, teleost fish and amphibians retain this capacity throughout life, leading to the use of the powerful zebrafish model system to identify novel mechanisms that promote spinal cord regeneration. While adult zebrafish offer an effective comparison with non-regenerating mammals, they lack the complete array of experimental approaches that have made this animal model so successful. In contrast, the optical transparency, simple anatomy and complex behavior of zebrafish larvae, combined with the known conservation of pro-regenerative signals and cell types between larval and adult stages, suggest that they may hold even more promise as a system for investigating spinal cord regeneration. In this review, we highlight characteristics and advantages of the larval model that underlie its potential to provide future therapeutic approaches for treating human spinal cord injury.

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