Controlled release of PEG chain from gold nanorods: Targeted delivery to tumor

Niidome, Takuro; Ohga, Akira; Akiyama, Yasuyuki; Watanabe, Kazuto; Niidome, Yasuro; Mori, Takeshi; Katayama, Yoshiki

doi:10.1016/j.bmc.2010.04.070

Cited by 39 publications

(34 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They found that the cytotoxicity of polyelectrolytes coated GNRs was relatively lower than that of CTABcapped GNRs. In addition, Niidome et al 1,54,55 reported PEG-modified GNRs using thiol-terminated methoxy-poly(ethylene glycol). Since PEG is a biocompatible polymer frequently used in drug and DNA delivery applications, PEG-modified GNRs showed around 95% cell viability at 0.05 mM concentration whereas less than 20% cells were viable in the presence of CTAB-stabilized GNRs.…”

Section: Synthesis and Biocompatibility Of Gnrsmentioning

confidence: 97%

Photothermal Cancer Therapy and Imaging Based on Gold Nanorods

et al. 2011

View full text Add to dashboard Cite

Gold nanorods (GNRs), which strongly absorb near-infrared (NIR) light, have shown great potential in fields of biomedical application. These include photothermal therapy, molecular imaging, biosensing, and gene delivery, especially for the treatment of diseased tissues such as cancer. These biomedical applications of GNRs arise from their various useful properties; photothermal (nanoheater) properties, efficient large scale synthesis, easy functionalization, and colloidal stability. In addition, GNRs do not decompose and have an enhanced scattering signal and tunable longitudinal plasmon absorption which allow them to be used as a stable contrast agent. Therefore, GNRs are also promising theranostic agents, combining both tumor diagnosis and treatment. In this review, we discuss the recent progress of in vitro and in vivo explorations of the diagnostic and therapeutic applications of GNRs as a component of cancer therapy.

show abstract

Section: Synthesis and Biocompatibility Of Gnrsmentioning

confidence: 97%

Photothermal Cancer Therapy and Imaging Based on Gold Nanorods

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Here, the PEG layer is attached to NP surface via a linker that may be cleaved by a condition unique to solid tumors such as weakly acidic environment (10, 11) or enzymes abundant in the tumor extracellular matrix such as matrix metalloproteinases (MMPs) (12–15). For example, liposomes with removable PEG were prepared using a PEG-peptide-phospholipid ternary conjugate, where the peptide was sensitive to MMPs.…”

Section: Introductionmentioning

confidence: 99%

“…These liposomes were used for the delivery of an anti-cancer drug (16), plasmid DNA (17), or siRNA (18), showing greater cellular uptake and biological activities with the removal of PEG by MMPs. The same principle was used in imaging agents such as quantum dots (19) or gold nanorods (12). These NPs were covered with a conjugate of PEG and MMP-substrate peptide via the avidin/biotin interaction (19) or a conjugate of PEG and a peptide substrate of urokinase-type plasminogen activator via gold-sulfur bond (12).…”

Section: Introductionmentioning

confidence: 99%

Polydopamine-Based Surface Modification for the Development of Peritumorally Activatable Nanoparticles

2013

View full text Add to dashboard Cite

Purpose To create a poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs), where a drug-encapsulating NP core is covered with polyethylene glycol (PEG) in a normal condition but exposes a cell-interactive TAT-modified surface in an environment rich in matrix metalloproteinases (MMPs). Methods PLGA NPs were modified with TAT peptide (PLGA-pDA-TAT NPs) or dual-modified with TAT peptide and a conjugate of PEG and MMP-substrate peptide (Peritumorally activatable NPs, PANPs) via dopamine polymerization. Cellular uptake of fluorescently-labeled NPs was observed with or without a pre-treatment of MMP-2 by confocal microscopy and flow cytometry. NPs loaded with paclitaxel (PTX) were tested against SKOV-3 ovarian cancer cells to evaluate the contribution of surface modification to cellular delivery of PTX. Results While the size and morphology did not significantly change due to the modification, NPs modified with dopamine polymerization were recognized by their dark color. Moreover, TAT-containing NPs (PLGA-pDA-TAT NPs and PANPs) showed changes in surface charge, indicative of effective conjugation of TAT peptide on the surface. PLGA-pDA-TAT NPs and MMP-2-pre-treated PANPs showed relatively good cellular uptake as compared to PLGA NPs, MMP-2-non-treated PANPs, and NPs with non-cleavable PEG. After 3 hour treatment with cells, PTX loaded in cell-interactive NPs showed greater toxicity than that in non-interactive ones as the former could enter cells during the incubation period. However, due to the initial burst drug release, the difference was not as clear as microscopic observation. Conclusions PEGylated polymeric NPs that exposed cell-interactive surface in response to MMP-2 were successfully created by dual modification of PLGA NPs using dopamine polymerization.

show abstract

“…Reasons for PEGylation (i.e. the covalent attachment of PEG) of surfaces nanoparticles are numerous and include shielding of antigenic and immunogenic epitopes, shielding receptor-mediated uptake by the reticuloendothelial system, and preventing recognition and degradation by proteolytic enzymes for biopolymers 10,[12][13][14] . GNR have high optoacoustic contrast, and can be conjugated with specific ligands, such as antibodies, to produce the targeted contrast agents 2 .…”

Section: Introductionmentioning

confidence: 99%

“…Demonstrations of photothermal cancer therapy using gold nanorods as a photothermal converter have also been reported by several groups. Targeting gold nanorods to a specific site is both a critical aspect of bioimaging using gold nanorods as a contrast agent, and for achieving efficient photothermal therapy without side effects especially after intravenous injection 13,16,18 . The standard for conjugating gold nanoparticles to antibodies using covalent bonding was published by utes, the ng to the Thermo med with and after mination was performed through measurement of absorbance at or near 562 nm.…”

Section: Introductionmentioning

confidence: 99%

PEG-coated gold nanorod monoclonal antibody conjugates in preclinical research with optoacoustic tomography, photothermal therapy, and sensing

2012

View full text Add to dashboard Cite

Gold nanorods (GNR) with a peak absorption wavelength of 760 nm were prepared using a seed-mediated method. A novel protocol has been developed to replace hexadecyltrimethylammonium bromide (CTAB) on the surface of GNR with 16-mercaptohexadecanoic acid (MHDA) and metoxy-poly(ethylene glycol)-thiol (PEG), and the monoclonal antibodies: HER2 or CD33. The physical chemistry property of the conjugates was monitored through optical and zetapotential measurements to confirm surface chemistry. The plasmon resonance is kept in the near infrared area, and changes from strong positive charge for GNR-CTAB to slightly negative for GNR-PEG-mAb conjugates are observed. The conjugates were investigated for different cells lines: breast cancer cells and human leukemia lines in vivo applications. These results demonstrate successful tumor accumulation of our modified PEG-MHDA conjugates of GNR for HER2/neu in both overexpressed breast tumors in nude mice, and for thermolysis of human leukemia cells in vitro. The conjugates are non-toxic and can be used in pre-clinical applications, as well as molecular and optoacoustic imaging, and quantitative sensing of biological substrates.

show abstract

Controlled release of PEG chain from gold nanorods: Targeted delivery to tumor

Cited by 39 publications

References 26 publications

Photothermal Cancer Therapy and Imaging Based on Gold Nanorods

Photothermal Cancer Therapy and Imaging Based on Gold Nanorods

Polydopamine-Based Surface Modification for the Development of Peritumorally Activatable Nanoparticles

PEG-coated gold nanorod monoclonal antibody conjugates in preclinical research with optoacoustic tomography, photothermal therapy, and sensing

Contact Info

Product

Resources

About