Controlled phage therapy by photothermal ablation of specific bacterial species using gold nanorods targeted by chimeric phages

Peng, Huan; Borg, Raymond E; Dow, Liam P.; Pruitt, Beth L.; Chen, Irene

doi:10.1073/pnas.1913234117

Cited by 100 publications

(122 citation statements)

References 55 publications

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“…This phage treatment was tested over P. aeruginosa biofilms, showing widespread bacterial cell death even when they were cultured in mammalian epithelial cells. Thus, combination of gold nanorods and genetically modified phages results in an interesting tool for biofilm removal [88].…”

Section: Genetically Modified Phagesmentioning

confidence: 99%

Phages for Biofilm Removal

2020

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Biofilms are clusters of bacteria that live in association with surfaces. Their main characteristic is that the bacteria inside the biofilms are attached to other bacterial cells and to the surface by an extracellular polymeric matrix. Biofilms are capable of adhering to a wide variety of surfaces, both biotic and abiotic, including human tissues, medical devices, and other materials. On these surfaces, biofilms represent a major threat causing infectious diseases and economic losses. In addition, current antibiotics and common disinfectants have shown limited ability to remove biofilms adequately, and phage-based treatments are proposed as promising alternatives for biofilm eradication. This review analyzes the main advantages and challenges that phages can offer for the elimination of biofilms, as well as the most important factors to be taken into account in order to design effective phage-based treatments.

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Section: Genetically Modified Phagesmentioning

confidence: 99%

Phages for Biofilm Removal

2020

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“…New methods for detecting and eliminating antibiotic-resistant, Gram-negative bacteria were developed by conjugating the phages to gold nanorods, whose excitation by near-infrared light at 808 nm causes localized heating (up to 81 °C) that is capable of destroying the bacteria nearby [61].…”

Section: Plasmonic Gold Nanoparticles For Bacteria and Biofilm Phototmentioning

confidence: 99%

Photothermally active nanoparticles as a promising tool for eliminating bacteria and biofilms

Borzenkov¹,

Pallavicini²,

Taglietti³

et al. 2020

Beilstein J. Nanotechnol.

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Bacterial contamination is a severe issue that affects medical devices, hospital tools and surfaces. When microorganisms adhere to a surface (e.g., medical devices or implants) they can develop into a biofilm, thereby becoming more resistant to conventional biocides and disinfectants. Nanoparticles can be used as an antibacterial agent in medical instruments or as a protective coating in implantable devices. In particular, attention is being drawn to photothermally active nanoparticles that are capable of converting absorbed light into heat. These nanoparticles can efficiently eradicate bacteria and biofilms upon light activation (predominantly near the infrared to near-infrared spectral region) due a rapid and pronounced local temperature increase. By using this approach new, protective, antibacterial surfaces and materials can be developed that can be remotely activated on demand. In this review, we summarize the state-of-the art regarding the application of various photothermally active nanoparticles and their corresponding nanocomposites for the light-triggered eradication of bacteria and biofilms.

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“…We can find more examples of how bacteriophages are being used for human or animal models, in addition to different bioengineering methods using bacteriophages that are currently being developed. [29][30][31] Bacteriophages Used for Accelerated Therapeutic Antibody Production Against the Virus Despite the fact that bacteriophages' potential to fight bacterial infections has only recently been rediscovered, they were successfully used as tools at the molecular level, leading to Nobel Prize awards. 32 Using a technique called phage display, bacteriophages have the potential to quickly produce recombinant antibodies.…”

Section: Natural Bacteriophages' Potential-a Direct Weapon Against Bamentioning

confidence: 99%

Bacteriophages Could Be a Potential Game Changer in the Trajectory of Coronavirus Disease (COVID-19)

Wojewodzic

2020

PHAGE

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The pandemic of the coronavirus disease (Covid-19) has caused the death of at least 270,000 people as of the 8th of May 2020. This work stresses the potential role of bacteriophages to decrease the mortality rate of patients infected by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus. The indirect cause of mortality in Covid-19 is miscommunication between the innate and adaptive immune systems, resulting in a failure to produce effective antibodies against the virus on time. Although further research is urgently needed, secondary bacterial infections in the respiratory system could potentially contribute to the high mortality rate observed among the elderly due to Covid-19. If bacterial growth, together with delayed production of antibodies, is a significant contributing factor to Covid-19's mortality rate, then the additional time needed for the human body's adaptive immune system to produce specific antibodies could be gained by reducing the bacterial growth rate in the respiratory system of a patient. Independently of that, the administration of synthetic antibodies against SARS-CoV-2 viruses could potentially decrease the viral load. The decrease of bacterial growth and the covalent binding of synthetic antibodies to viruses should further diminish the production of inflammatory fluids in the lungs of patients (the indirect cause of death). Although the first goal could potentially be achieved by antibiotics, I argue that other methods may be more effective or could be used together with antibiotics to decrease the growth rate of bacteria, and that respective clinical trials should be launched. Both goals can be achieved by bacteriophages. The bacterial growth rate could potentially be reduced by the aerosol application of natural bacteriophages that prey on the main species of bacteria known to cause respiratory failure and should be harmless to a patient. Independently of that, synthetically changed bacteriophages could be used to quickly manufacture specific antibodies against SARS-CoV-2. This can be done via a Nobel Prize awarded technique called ''phage display.'' If it works, the patient is given extra time to produce their own specific antibodies against the SARS-CoV-2 virus and stop the damage caused by an excessive immunological reaction.

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Controlled phage therapy by photothermal ablation of specific bacterial species using gold nanorods targeted by chimeric phages

Cited by 100 publications

References 55 publications

Phages for Biofilm Removal

Phages for Biofilm Removal

Photothermally active nanoparticles as a promising tool for eliminating bacteria and biofilms

Bacteriophages Could Be a Potential Game Changer in the Trajectory of Coronavirus Disease (COVID-19)

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