Controlled heparin conjugation on electrospun poly(ε-caprolactone)/gelatin fibers for morphology-dependent protein delivery and enhanced cellular affinity

Lee, J.; Yoo, James J.; Atala, Anthony; Lee, S.J.

doi:10.1016/j.actbio.2012.03.030

Cited by 51 publications

(46 citation statements)

References 42 publications

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“…www.ecmjournal.org have been shown to successfully retain PDGF-BB bioactivity and allow for sustained delivery, compared to physically adsorbed PDGF-BB. The same delivery devices, with physically adsorbed PDGF-BB, exhibited a burst release of the growth factor within the first 3-4 d (Lee et al, 2012a;Sun et al, 2009). Studies on tendon explants have shown results similar to cell cultures.…”

Section: Fibrin-based Delivery Devicessupporting

confidence: 52%

“…Heparin conjugation with electrospun polymeric fibres with subsequent PDGF-BB loading ( Fig. 5A) has been done in only a few studies using poly (ε-caprolactone) (PCL)/gelatin fibres (Lee et al, 2012b), poly(L-lactide) fibres (plasma assisted heparin conjugation) (Cheng et al, 2014) and PCL/gelatin electrospun fibres (Lee et al, 2012a;Lee et al, 2012b), where cellular bioactivity and cell infiltration were studied.…”

Section: Heparin-conjugated Systemsmentioning

confidence: 99%

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In vitro and in vivo effects of PDGF-BB delivery strategies on tendon healing: a review

Evrova

Buschmann

2017

eCM

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To promote and support tendon healing, one viable strategy is the use or administration of growth factors at the wound/rupture site. Platelet derived growth factor-BB (PDGF-BB), together with other growth factors, is secreted by platelets after injury. PDGF-BB promotes mitogenesis and angiogenesis, which could accelerate tendon healing. Therefore, in vitro studies with PDGF-BB have been performed to determine its effect on tenocytes and tenoblasts. Moreover, accurate and sophisticated drug delivery devices, aiming for a sustained release of PDGF-BB, have been developed, either by using heparin-binding and fibrin-based matrices or different electrospinning techniques.In this review, the structure and composition, as well as the healing process of tendons, are described. Part A deals with in vitro studies. They focus on the multiple effects evoked by PDGF-BB on the cellular level. Moreover, they address strategies for the sustained delivery of PDGF-BB. Part B focuses on animal models used to test different delivery strategies for PDGF-BB, in the context of tendon reconstruction. These studies showed that dosage and timing of PDGF-BB application are the most important factors for deciding which delivery device should be applied for a specific tendon laceration.

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Section: Fibrin-based Delivery Devicessupporting

confidence: 52%

Section: Heparin-conjugated Systemsmentioning

confidence: 99%

In vitro and in vivo effects of PDGF-BB delivery strategies on tendon healing: a review

Evrova

Buschmann

2017

eCM

View full text Add to dashboard Cite

show abstract

“…We heparinized gelatin sponge as described previously [30,31], and used a toluidine blue assay and SEM observation [30] to compare heparinized gelatin sponge to a normal (nonheparinzed) gelatin sponge (Fig. 6).…”

Section: Functionalization Of Gelatin Spongementioning

confidence: 99%

“…Heparin was immobilized on the gelatin sponge as described previously [30,31]. Briefly, absorbable gelatin sponge (gelfoam, Pfizer Inc., NY, USA) was equilibrated in 0.05 M 2-morpholinoethane sulfonic acid buffer (MES, pH 5.6) for During this step, the carboxyl groups of heparin were chemically immobilized with the amine groups of gelatin.…”

Section: Heparinization Of Gelatin Spongementioning

confidence: 99%