Controlled Field Release of a Bioluminescent Genetically Engineered Microorganism for Bioremediation Process Monitoring and Control

Ripp, Steven; Nivens, David E.; Ahn, Yeonghee; Werner, Cláudia Maria Lima; Jarrell,; Easter, J.; Cox, Chris D.; Burlage, Robert S.; Sayler, Gary S.

doi:10.1021/es002004+

Cited by 8 publications

(7 citation statements)

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“…luciferase and β-galactosidase) that emit visible light in response to the presence of specific substances [72]. Several biosensors using this promoter-reporter-gene concept have been developed for the detection of heavy metals [73,74,75] and toxic organic compounds [76,77,78].…”

Section: Microorganismsmentioning

confidence: 99%

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Fiber-optic biosensors – an overview

2002

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This article reviews progress and developments during the past five years in the field of optical fiber biosensors. Because of the expense and time constraints associated with modern laboratory analysis, there is a growing need for real-time, low-cost technology that can be used industrially, environmentally, and clinically, and to monitor food processing. Miniaturization, integrated systems, and multianalyte determination have become key aspects of sensor development and efforts in this direction will also be discussed, with some pointers to likely directions of future research in the area. The review will provide information about the analytical characteristics and applications of fiber-optic biosensors classified depending on the biorecognition element employed - enzymes, whole cells, antibodies, nucleic acids, and biomimetic polymers.

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Section: Microorganismsmentioning

confidence: 99%

“…naphthalene. Fiber-optic biosensors utilizing alginate-encapsulated HK44 cells have been used to monitor the presence of naphthalene in soil bioremediation studies [77]. The estimated lifetime of these biosensors is approximately one week; after that they must be replaced with newly encapsulated cells.…”

Section: Microorganismsmentioning

confidence: 99%

Fiber-optic biosensors – an overview

2002

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“…Fusions of lux to this promoter induce luminescence in response to naphthalene, salicylate, and some other aromatic compounds [169]. One such example, Pseudomonas fluorescens HK44, has been released into the field for in situ monitoring of a bioremediation process [170]. The authors described this as the first genetically modified microorganism approved for field testing in bioremediation experiments in the United States.…”

Section: Naphthalene Detectionmentioning

confidence: 99%

Wastewater Toxicity Assessment by Whole Cell Biosensor

Philp

French

Wiles

et al. 2004

The Handbook of Environmental Chemistry

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“…Although GMMs have been used for 20 years in the production of pharmaceuticals, vaccines, and enzymes, none of these applications involves the release of GMMs or viruses (31) and only a few GMMs have been approved for release into the environment (8,27,32,35).…”

mentioning

confidence: 99%

“Mark the Gene”: a Method for Nondestructive Introduction of Marker Sequences Inside the Gene Frame of Transgenes

Morono

Kitagawa

Kimura

et al. 2007

Appl Environ Microbiol

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A specific marking and detection technique is a fundamental requirement for the safer use of genetically modified (GM) organisms. Here we propose a simple and effective method for directly marking functional transgenes in GM organisms. For that purpose, we introduced nucleotide substitutions (NS), based on the degeneracy of codons as markers (NS markers), into the bphC (2,3-dihydroxybiphenyl dioxygenase) and tomA3 (toluene-ortho-monooxygenase) gene frames using a PCR-based method. No change was observed in the enzyme activity of translated proteins, and alignments with homologous genes showed the uniqueness of the NS markers. Furthermore, we constructed tomA3 variations harboring NS markers in different positions. Although the translational products were identical, the constructed variation genes could be distinguished through their marker patterns by multiplex PCR, showing that NS markers could serve as product-specific tags for identifying individual GM organisms. This direct method of marking the functional transgene provides a simple, low-risk, and robust marking method without causing the gene functions to deteriorate.

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Controlled Field Release of a Bioluminescent Genetically Engineered Microorganism for Bioremediation Process Monitoring and Control

Cited by 8 publications

References 0 publications

Fiber-optic biosensors – an overview

Fiber-optic biosensors – an overview

Wastewater Toxicity Assessment by Whole Cell Biosensor

“Mark the Gene”: a Method for Nondestructive Introduction of Marker Sequences Inside the Gene Frame of Transgenes

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