Wastewater Toxicity Assessment by Whole Cell Biosensor

Philp, Jim; French, Christopher C.; Wiles, Sioxsie; Bell, Jordan; Whiteley, Andrew S.; Bailey, Mark

doi:10.1007/b97179

Cited by 5 publications

(2 citation statements)

References 181 publications

(219 reference statements)

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“…This type of biosensor is based on the highly specific genetic control mechanisms used by micro-organisms to ensure that specific proteins are only expressed when they are needed; for example, for the detoxification of a particular toxic substance. Biosensors of this type are easily generated (Philp et al, 2004). The most popular reporter is the bacterial lux system, which produces light, because light can be easily quantified and, due to its rarity in biology, there is no interference from the background biochemistry of the host organism.…”

Section: Indirect Biological Methodsmentioning

confidence: 99%

Biotechnology for the Environment in the Future

2013

OECD Science, Technology and Industry Policy Papers

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The OECD Directorate for Science, Technology and Industry (www.oecd.org/sti) develops evidence-based policy advice on the contribution of science, technology and industry to wellbeing and economic growth. STI Policy Papers cover a broad range of topics, including industry and globalisation, innovation and entrepreneurship, scientific R&D and emerging technologies. These reports are officially declassified by an OECD Committee.

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Section: Indirect Biological Methodsmentioning

confidence: 99%

Biotechnology for the Environment in the Future

2013

OECD Science, Technology and Industry Policy Papers

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“…• Sowohl Mikroorganismen, die natürliche Biolumineszenz aufweisen, als auch gentechnisch modifizierte biolumineszente Zellsysteme ändern ihre Biolumineszenz in Gegenwart biologisch aktiver Substanzen. So lässt die Lichtemission der natürlichen marinen Leuchtbakterien Vibrio fischeri nach, wenn sie mit Substanzen, die die Atmungskette schädigen, in Kontakt kommen; sie weisen so toxische Substanzen empfindlich nach 1…”

Section: Biolumineszenz Als Detektorsignalunclassified

Biologische Wirkung direkt messen

Kreiss¹,

Ebertz²,

Weisemann³

2007

Nachr Chem

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Upgrading bioluminescent bacterial bioreporter performance by splitting the lux operon

Yagur‐Kroll

Belkin

2010

Anal Bioanal Chem

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Bioluminescent bacterial bioreporters harbor a fusion of bacterial bioluminescence genes (luxCDABE), acting as the reporting element, to a stress-response promoter, serving as the sensing element. Upon exposure to conditions that activate the promoter, such as an environmental stress or the presence of an inducing chemical, the promoter::reporter fusion generates a dose-dependent bioluminescent signal. In order to improve bioluminescent bioreporter performance we have split the luxCDABE genes of Photorhabdus luminescens into two smaller functional units: luxAB, that encode for the luciferase enzyme, which catalyzes the luminescence reaction, and luxCDE that encode for the enzymatic complex responsible for synthesis of the reaction's substrate, a long-chain aldehyde. The expression of each subunit was put under the control of either an inducible stress-responsive promoter or a synthetic constitutive promoter, and different combinations of the two units were tested for their response to selected chemicals in Escherichia coli. In all cases tested, the split combinations proved to be superior to the native luxCDABE configuration, suggesting an improved efficiency in the transcription and/or translation of two small gene units instead of a larger one with the same genes. The best combination was that of an inducible luxAB and a constitutive luxCDE, indicating that aldehyde availability is limited when the five genes are expressed together in E. coli, and demonstrating that improved biosensor performance may be achieved by rearrangement of the lux operon genes.

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Wastewater Toxicity Assessment by Whole Cell Biosensor

Cited by 5 publications

References 181 publications

Biotechnology for the Environment in the Future

Biotechnology for the Environment in the Future

Biologische Wirkung direkt messen

Upgrading bioluminescent bacterial bioreporter performance by splitting the lux operon

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