Controlled Elimination of Intracellular H<sub>2</sub>O2: Regulation of Peroxiredoxin, Catalase, and Glutathione Peroxidase via Post-translational Modification

Rhee, Sue Goo; Yang, Kap Seok; Kang, Sang Won; Woo, Hyun Ae; Chang, Tong Shin

doi:10.1089/ars.2005.7.619

Cited by 343 publications

(245 citation statements)

References 56 publications

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“…However, the activity of catalase was decreased in the femoral arteries from STZ-treated rats despite an increased protein presence of the enzyme. Catalase is not efficient in eliminating low levels of hydrogen peroxide because it is difficult to saturate with hydrogen peroxide and its catalytic activity requires the interaction of two hydrogen peroxide molecules with a single active site, which is less likely to occur at low hydrogen peroxide concentrations (Halliwell and Gutteridge, 2000;Rhee et al, 2005). This further suggests that the accumulation of hydrogen peroxide in these arteries can be mainly attributed to a reduced activity of catalase.…”

Section: Discussionmentioning

confidence: 99%

Oxygen‐derived free radicals mediate endothelium‐dependent contractions in femoral arteries of rats with streptozotocin‐induced diabetes

Shi

Man

et al. 2007

British J Pharmacology

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Background and purpose:The present experiments were designed to study the contribution of oxygen-derived free radicals to endothelium-dependent contractions in femoral arteries of rats with streptozotocin-induced diabetes. Experimental approach: Rings with and without endothelium were suspended in organ chambers for isometric tension recording. The production of oxygen-derived free radicals in the endothelium was measured with 2 0 ,7 0 -dichlorodihydrofluorescein diacetate using confocal microscopy. The presence of protein was measured by western blotting. Key results: In the presence of L-NAME, the calcium ionophore A23187 induced larger endothelium-dependent contractions in femoral arteries from diabetic rats. Tiron, catalase, deferoxamine and MnTMPyP, but not superoxide dismutase reduced the response, suggesting that oxygen-derived free radicals are involved in the endothelium-dependent contraction. In the presence of L-NAME, A23187 increased the fluorescence signal in femoral arteries from streptozotocin-treated, but not in those from control rats, confirming that the production of oxygen-derived free radicals contributes to the enhanced endotheliumdependent contractions in diabetes. Exogenous H 2 O 2 caused contractions in femoral arterial rings without endothelium which were reduced by deferoxamine, indicating that hydroxyl radicals contract vascular smooth muscle and thus could be an endothelium-derived contracting factor in diabetes. The reduced presence of Mn-SOD and the decreased activity of catalase in femoral arteries from streptozotocin-treated rats demonstrated the presence of a redox abnormality in arteries from rats with diabetes. Conclusions and implications: These findings suggest that the redox abnormality resulting from diabetes increases oxidative stress which facilitates and/or causes endothelium-dependent contractions.

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Section: Discussionmentioning

confidence: 99%

Oxygen‐derived free radicals mediate endothelium‐dependent contractions in femoral arteries of rats with streptozotocin‐induced diabetes

Shi

Man

et al. 2007

British J Pharmacology

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“…Two days after the change of diet, a cluster of stress response proteins has been found to be modulated, of which TXNDC5 and PRDX6 are important antioxidant enzymes protecting cells against oxidative injury by reducing endogenous levels of peroxides. Both proteins catalyze the degradation of H 2 O 2 , organic hydroperoxides, and peroxinitrites and are involved in signal transduction, cell proliferation, differentiation, and apoptosis (51,65). It is well known that immune cells can produce large amounts of ROMs, usually referred as "reactive oxygen metabolites," when stimulated.…”

Section: G265 Diet-dependent Protein Expression Of Ruminal Epitheliummentioning

confidence: 99%

Identification of differentially expressed proteins in ruminal epithelium in response to a concentrate-supplemented diet

Bondzio¹,

Gabler²,

Badewien-Rentzsch³

et al. 2011

American Journal of Physiology-Gastrointestinal and Liver Physi

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minal epithelium adapts to dietary change with well-coordinated alterations in metabolism, proliferation, and permeability. To further understand the molecular events controlling diet effects, the aim of this study was to evaluate protein expression patterns of ruminal epithelium in response to various feeding regimes. Sheep were fed with a concentrate-supplemented diet for up to 6 wk. The control group received hay only. Proteome analysis with differential in gel electrophoresis technology revealed that, after 2 days, 60 proteins were significantly modulated in ruminal epithelium in a comparison between hay-fed and concentrate-fed sheep (P Ͻ 0.05). Forty proteins were upregulated and 20 proteins were downregulated in response to concentrate diet. After 6 wk of this diet, only 14 proteins were differentially expressed. Among these, 11 proteins were upregulated and 3 downregulated. To identify proteins that were modulated by dietary change, two-dimensional electrophoresis was coupled with liquid chromatography electrospray ionization mass spectrometry. The differential expression of selected proteins, such as esterase D, annexin 5, peroxiredoxin 6, carbonic anhydrase I, and actin-related protein 3, was verified by immunoblotting and/or mRNA analysis. The identified proteins were mainly associated with functions related to cellular stress, metabolism, and differentiation. These results suggest new candidate proteins that may contribute to a better understanding of the signaling pathways and mechanisms that mediate rumen epithelial adaptation to high-concentrate diet.proteomics; mRNA expression; protein markers; sheep RUMINANTS ARE OF MAJOR WORLDWIDE interest and economic importance because of increasing meat and milk production. A substantial improvement in the productivity and performance of ruminants can only be achieved with adequate dietary supplementation. When high-yielding cattle, milk sheep, or goats merely consume forage, the intake of energy and protein is too low for the desired rates of animal performance. Hence, supplementation of forage-fed ruminants with sufficient amounts of energy and protein is necessary and significantly improves milk production or daily weight gain (41), but, particularly, a surplus of easily fermented carbohydrates or low-fiber intake will place the animals at risk. Such a change in the diet is a challenge for rumen microbes (31) and especially for the ruminal epithelium with respect to adaptation to the new fermentation pattern (47,55). Insufficient adaptation to diet may result in disintegration of the epithelium (22), translocation of lipopolysaccharides (30), and disturbed transport mechanisms (22) and can further cause subacute ruminal acidosis (14, 49). This disease not only can lead to depressed feed intake and milk production, but can also be associated with laminitis, inflammation, and liver abscesses (14,42,49).Hence, one objective of attaining an adequate diet composition for ruminants is to maintain the physiological functions of the forestomach, such as optimal fe...

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“…ARE driven expression of detoxifying and antioxidant enzymes and the cystine/glutamate transporter involved in GSH biosynthesis [121,122].…”

Section: Protection Against Oxidative Stress and "Igf-i Deficiency Comentioning

confidence: 99%

Protection Against Oxidative Stress and “IGF-I Deficiency Conditions”

Morón¹,

Castilla‐Cortázar²

2012

Antioxidant Enzyme

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Controlled Elimination of Intracellular H₂O2: Regulation of Peroxiredoxin, Catalase, and Glutathione Peroxidase via Post-translational Modification

Cited by 343 publications

References 56 publications

Oxygen‐derived free radicals mediate endothelium‐dependent contractions in femoral arteries of rats with streptozotocin‐induced diabetes

Oxygen‐derived free radicals mediate endothelium‐dependent contractions in femoral arteries of rats with streptozotocin‐induced diabetes

Identification of differentially expressed proteins in ruminal epithelium in response to a concentrate-supplemented diet

Protection Against Oxidative Stress and “IGF-I Deficiency Conditions”

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Controlled Elimination of Intracellular H2O2: Regulation of Peroxiredoxin, Catalase, and Glutathione Peroxidase via Post-translational Modification

Cited by 343 publications

References 56 publications

Oxygen‐derived free radicals mediate endothelium‐dependent contractions in femoral arteries of rats with streptozotocin‐induced diabetes

Oxygen‐derived free radicals mediate endothelium‐dependent contractions in femoral arteries of rats with streptozotocin‐induced diabetes

Identification of differentially expressed proteins in ruminal epithelium in response to a concentrate-supplemented diet

Protection Against Oxidative Stress and “IGF-I Deficiency Conditions”

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Product

Resources

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Controlled Elimination of Intracellular H₂O2: Regulation of Peroxiredoxin, Catalase, and Glutathione Peroxidase via Post-translational Modification