Controlled degradation of low-fouling poly(oligo(ethylene glycol)methyl ether methacrylate) hydrogels

Abstract

To tune degradation rates of low-fouling hydrogels, a 6-member P(EG)xMA copolymer library with different electronics and hydration levels was developed.

The Removal of Hydrophobic Matter from Thermosensitive Poly[oligo(ethylene glycol) Monomethyl Ether Acrylate] Gel Adsorbent in Alcohol–Water Mixtures

“…Scheme 1 shows the novel process for removing hydrophobic impurities from dilute aqueous solutions using POEG(M)A porous gels, which involves regenerating shrunken gels by exploiting UCST-type transition behavior; these gels have high affinities for a variety of chemicals. POEG(M)A gels shrunken below the LCST are reportedly useful adsorbers of chemicals, such as petroleum oil [12], protein [36], and dye [37] by hydrophobic interaction as we expressed in our previous study [12]. The BPA is a hydrophobic organic material [38] and can be adsorbed by thermosensitive gel [39] including PEGDA [40] when the gel is hydrophobic.…”

Modulating the Thermoresponse of Polymer-Protein Conjugates with Hydrogels for Controlled Release

“…Hydrogel degradation will promote the solubilization of the conjugate for greater release rates and eventual complete release. The pCB-Az copolymer for crosslinking with pCB-DBCO was modified to contain β eliminative carbamate bonds with reported half-lives of 36 h ( Figure 6 a); the hydrogel crosslinks will therefore hydrolyze for complete hydrogel degradation [ 15 , 16 , 33 ]. To demonstrate degradability, the hydrogel was formed in a pre-weighed microcentrifuge tube and wet weight was followed over time ( Figure 6 b); wet weight increases at first due to hydrogel swelling.…”

“…3-(3-dimethylaminopropyl)-1-ethyl-carbodiimide hydrochloride (EDC-HCl) was obtained from Chem-Impex International Inc. (Wood Dale, IL, USA) Alexa Fluor 647 NHS ester, Alexa Fluor 488 NHS Ester, Fetal bovine serum (FBS)DMEM, Alamar blue was purchased from Thermo Fisher Scientific (Burlington, ON, Canada). O-(6-azidohexyl)-O’-succinimidyl carbonate (NHS-azide), O-[1-(4-chlorophenylsulfonyl)-7-azido-2-heptyl]-O′-succinimidylcarbonate (NHS-AzCl) and carboxybetaine monomer were synthesized as previously described [ 15 , 16 , 17 ].…”

“…Poly(carboxybetaine) (pCB) hydrogel synthesis. pCB hydrogels were synthesized in a similar manner as previously described [ 15 , 17 , 18 ]. Copolymers of pCB-APMA, pCB-Azide (pCB-Az), pCB-AzCl and pCB-DBCO were synthesized as described below.…”