Controllable Nitric Oxide Release in the Presence of Gold Nanoparticles

Abstract

A major problem associated with nitric oxide (NO) donors is the release of the desired amount of NO at a specific site. A number of platforms have been developed for the regulation of NO dosage. We present the use of citrate-stabilized gold nanoparticles as a platform to regulate NO release. Because of the affinity between gold and thiols, the characteristic -S-NO bond of S-nitrosothiols (RSNOs) breaks in the presence of gold nanoparticles, thereby releasing NO and modifying the gold nanoparticle surface with the corresponding thiol. This system allows for surface-controlled NO release, where the amount of NO released is proportional to the number of thiols bound to the gold nanoparticle surface. Moreover, by employing an amperometric technique to detect the maximum NO release, we were able to estimate the stoichiometry of the reaction, that is, the number of adsorbed RSNO molecules per gold nanoparticle. A kinetic model for NO release and its subsequent decomposition is proposed and used to fit the experimental results. The reaction was found to be zeroth- and first-order with respect to RSNO and gold nanoparticles, respectively.

Electrospun PCL/keratin/AuNPs mats with the catalytic generation of nitric oxide for potential of vascular tissue engineering

“…Interestingly, PCL/keratin/AuNPs mats in the presence of GSNO displayed further lower OD values, caused by the synergistic bactericidal effects of AuNPs and NO generated from GSNO. It was known that AuNPs could catalyze the decomposition of GSNO, resulting in the NO generation . Thus, the generating NO enhanced the antibacterials’ activity of AuNPs.…”

“…Metal nanoparticles, especially AuNPs, are highly attractive because of their unique electronic, optical, and catalytic properties . Au nanoparticles were demonstrated to catalyze NO regeneration from endogenous RSNOs . AuNPs are biocompatible and important for biomedical applications including computer tomography (CT) contrast agent and nanosensors .…”

“…It was known that AuNPs could catalyze the decomposition of GSNO, resulting in the NO generation. 21,22 Thus, the generating NO enhanced the antibacterials' activity of AuNPs.…”

“…Au nanoparticles were demonstrated to catalyze NO production from endogenous RSNOs. 21,22 Our previous results demonstrate that keratins are able to catalyze the blood endogenous NO donor to generate NO. 23 In the present study, poly(ε-caprolactone) (PCL) and keratins were first coelectrospun to afford biocomposite mats ( Fig.…”

“…Gold (Au) is regarded as one of the noble metals with high biocompatibility. Au nanoparticles were demonstrated to catalyze NO production from endogenous RSNOs …”