Control of viral haemorrhagic disease of rabbits in Poland

Gorski, Jack; Mizak, B.; Chrobocińska, M.

doi:10.20506/rst.13.3.802

Cited by 9 publications

(12 citation statements)

References 12 publications

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“…The first two cases of RHD in Poland were officially diagnosed in the spring of 1988 (20). The clinical symptoms of infection and pathological findings were similar to the description of RHD outbreaks reported at that time in neighbouring countries, and previously in China (29,33,35).…”

Section: Introductionsupporting

confidence: 79%

Evaluation of the usefulness of laboratory diagnostic methods in RHD outbreak

Fitzner

2014

Bulletin of the Veterinary Institute in Pulawy

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The field outbreak of RHD that occurred late summer 2012 on a small-scale rabbit-rearing operation in Poland and the usefulness of techniques for RHD virus diagnosis are described. During the epizootic, the overall mortality rate of rabbits older than two months was 77%. Eight liver specimens collected from dead unvaccinated rabbits (aged 3-5 months) underwent virological examinations. RHDV specific antigen was detected in two out of eight liver homogenates by haemagglutination (HA) test and ELISA, one of the two being negative in HA assay. However the presence of genetic material of RHD virus was confirmed by RT-PCR and real-time RT-PCR in all liver samples tested. Based on antigen reactivity in ELISA and sequencing of PCR amplicons of the VP60 gene, the RHDVa subtype strain was identified as the cause of infection. The partial genome sequence of a field isolate (STR 2012), comprising the C-terminus of the polymerase gene and the full capsid protein gene, revealed 91% nucleotide homology to reference FRG89 RHDV isolate and 97% to strain Triptis representing the RHDVa variant. Serological evidence of an RHD outbreak in the STR rabbit-rearing operation was confirmed in a serum sample collected from an unvaccinated surviving rabbit. A cross-reactivity examination of RHDV positive serum revealed a decrease in HI titre against the STR 2012 field antigen, and a decrease in the RHDVa control antigen as compared to classic RHDV.

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Section: Introductionsupporting

confidence: 79%

Evaluation of the usefulness of laboratory diagnostic methods in RHD outbreak

Fitzner

2014

Bulletin of the Veterinary Institute in Pulawy

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“…Exposure to 2% Virkon for 2 h does not inactivate the virus, although 4% Virkon is effective; 1% sodium hydroxide or 10% household bleach are also effective disinfectants (Gorski et al, 1994;Goodly, 2001). It is caused by a host-specific calicivirus (see Section 16.6.2.).…”

Section: Viral Haemorrhagic Diseasementioning

confidence: 99%

“…Controlled exposure to infection in the laboratory suggested that rabbits that were simultaneously vaccinated were immune to both diseases (Gorski et al, 1994). There are data to support the efficacy of simultaneous vaccination but no firm conclusions can be drawn because of differences in the type of vaccine.…”

Section: Simultaneous Administration Of Myxomatosis and Vhd Vaccinementioning

confidence: 99%

The rabbit consultation and clinical techniques

Harcourt-Brown¹

2002

Textbook of Rabbit Medicine

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“…Readers interested in additional information on this topic are referred to certain of the references [8,9,10,12,18,19,20,22,30,31]. …”

Section: Resultsmentioning

confidence: 99%

“…There is not an extensive literature dealing with low pH inactivation of caliciviruses, but that which could be found [6,9,12,21,31,75] is summarized in Table 9.…”

Section: Resultsmentioning

confidence: 99%

Inactivation of Caliciviruses

Nims¹,

Plavsic²

2013

Pharmaceuticals

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The Caliciviridae family of viruses contains clinically important human and animal pathogens, as well as vesivirus 2117, a known contaminant of biopharmaceutical manufacturing processes employing Chinese hamster cells. An extensive literature exists for inactivation of various animal caliciviruses, especially feline calicivirus and murine norovirus. The caliciviruses are susceptible to wet heat inactivation at temperatures in excess of 60 °C with contact times of 30 min or greater, to UV-C inactivation at fluence ≥30 mJ/cm2, to high pressure processing >200 MPa for >5 min at 4 °C, and to certain photodynamic inactivation approaches. The enteric caliciviruses (e.g.; noroviruses) display resistance to inactivation by low pH, while the non-enteric species (e.g.; feline calicivirus) are much more susceptible. The caliciviruses are inactivated by a variety of chemicals, including alcohols, oxidizing agents, aldehydes, and β-propiolactone. As with inactivation of viruses in general, inactivation of caliciviruses by the various approaches may be matrix-, temperature-, and/or contact time-dependent. The susceptibilities of the caliciviruses to the various physical and chemical inactivation approaches are generally similar to those displayed by other small, non-enveloped viruses, with the exception that the parvoviruses and circoviruses may require higher temperatures for inactivation, while these families appear to be more susceptible to UV-C inactivation than are the caliciviruses.

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Control of viral haemorrhagic disease of rabbits in Poland

Cited by 9 publications

References 12 publications

Evaluation of the usefulness of laboratory diagnostic methods in RHD outbreak

Evaluation of the usefulness of laboratory diagnostic methods in RHD outbreak

The rabbit consultation and clinical techniques

Inactivation of Caliciviruses

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