Control of protein orientation in molecular photoelectric devices using Langmuir—Blodgett films of photosynthetic reaction centers from Rhodopseudomonas viridis

Yasuda, Yoshiaki; Sugino, Hiroaki; Toyotama, Hideki; Hirata, Yoshiki; Hara, Masayuki; Miyake, Jun

doi:10.1016/0302-4598(94)80027-8

Cited by 54 publications

(18 citation statements)

References 10 publications

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“…Illumination of a cell that was constructed using a mixture of RC–LH1 complexes and TMPD produced a short‐circuit current density ( J SC ) that stabilized at a value of 0.15 μA cm −2 after approximately 20 s (Figure 1 a); the direction of the current indicates electron flow into the cell cavity from the FTO‐glass front electrode. The initial spike of current flow was similar to a feature that has been seen in photoelectric measurements on RCs in planar lipid bilayers13 and Langmuir Blodgett films 14. The initial spike was consistently seen in multiple cells, and its origins are considered below.…”

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confidence: 66%

Generation of Alternating Current in Response to Discontinuous Illumination by Photoelectrochemical Cells Based on Photosynthetic Proteins

et al. 2012

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Proteinbasierte photoelektrochemische Zellen, die unter Verwendung eines photosynthetischen Reaktionszentrums (links) hergestellt wurden, erzeugen konventionellen Gleichstrom bei kontinuierlicher Bestrahlung und Wechselstrom bei regelmäßigen Licht‐An/Licht‐Aus‐Zyklen (rechts). Der Mechanismus der Wechselstromerzeugung nutzt die Fähigkeit der natürlichen Reaktionszentren zur Ladungsspeicherung und zur Katalyse hocheffizienter photochemischer Ladungstrennung.

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confidence: 66%

Generation of Alternating Current in Response to Discontinuous Illumination by Photoelectrochemical Cells Based on Photosynthetic Proteins

et al. 2012

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“…64,65,66 Langmuir Blodgett (LB) method was widely being used to coat electrodes with RC layers 30 and attempts were made to control the orientation of the RCs on the electrodes. [67][68][69][70][71] The LB method involves consecutive crossing of an air-water interface by which a compact monomolecular layer of amphiphilic molecules is coated on the substrate with a well-defined molecular arrangement and orientation. 64 Artificial 35 lipid bilayers called liposomes were also used to immobilize the proteins on the electrode as the liposome film on the electrode can provide nearly native environment for the proteins.…”

Section: Ways To Interface Rcs To Electrodesmentioning

confidence: 99%

Progress and perspectives in exploiting photosynthetic biomolecules for solar energy harnessing

Ravi

Tan

2015

Energy Environ. Sci.

104

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5Photosynthetic proteins are emerging as a new class of photovoltaic materials as their nature-designed architecture and internal circuitry are so sophisticated that they carry out the initial light-driven steps of photosynthesis with ≈ 100% quantum efficiency. Research on bioinspired solar cells has increased in recent years as they promise better efficiency than the conventional p-n junction solar cells that have limited conversion efficiency (34%). Since it is a mammoth task to perfectly mimic the intricate proteins 10 evolved in nature, the idea of interfacing the natural proteins with engineered materials seems propitious for developing biohybrid solar cells. Herein, we summarize various approaches in immobilizing the photosynthetic biomolecules in photovoltaic devices and the progress in the photocurrent generation achieved. This review highlights the multidisciplinary nature of photosynthetic biohybrid devices and their future prospects in light of some of the research challenges and discrepancies witnessed by this field. 15 The fascinating aspect of this research area is that it guides the biologists to explore the possibilities of improving protein stability and robustness suitable for solar cells and inspires the solar cell researchers to explore the physics behind the working mechanisms of biohybrid solar cells which can generate novel architectures in future solar energy conversion devices. 60 superior system and mechanism for light harvesting and energy conversion. Their quantum efficiencies 17 are higher than that of the manmade solar cells. [18][19][20] Photosynthesis is an exemplary model for solar cell research as it is the prime mover powering the biological world, the mechanism behind the energy storage in 65 fossil fuels and the sustainer of earth's oxygenated atmosphere. 21 The architecture and the internal circuitry of the photosynthetic systems are very sophisticated that the initial light driven steps have ≈ 100% quantum efficiency. 18,22,23 Bioinspiration of photosynthesis in solar cells has instigated novel research 70 perspectives which are, in close pace, moving towards devising a high efficiency solar cell. Artificial Photosynthesis is one novel approach that tries to emulate the natural photosynthetic systems REVIEW This journal is © The Royal Society of Chemistry [year] [journal], [year], [vol], 00-00 | 9Fig. 7 (a) Two possible ways of RC binding and ET pathways between RC and electrode. P-primary electron donor (special pair), B-monomeric bacteriochlorophyll, H-bacteriopheophytin, Qa and Qb-primary and secondary electron acceptors (quinones). [Adapted from ref. 81 and ref.34, with permission from Elsevier Ltd] (b) Photoinduced-and dark-electron transfers in RC-Cyt-SAM-Gold electrode [Adapted with permission from ref.82,

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“…The initial spike of current flow was similar to a feature that has been seen in photoelectric measurements on RCs in planar lipid bilayers [13] and Langmuir Blodgett films. [14] The initial spike was consistently seen in multiple cells, and its origins are considered below. The open-circuit voltage (V OC ) of the RC-LH1 cell also showed an initial small spike (Figure 1 b), settling over the next approximately 20 s to a steady value of around 7 mV.…”

mentioning

confidence: 99%

Generation of Alternating Current in Response to Discontinuous Illumination by Photoelectrochemical Cells Based on Photosynthetic Proteins

et al. 2012

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There is developing interest in the device properties of natures chlorophyll-containing photochemical reaction centers (RCs) driven by the need to establish a range of technologies for harvesting solar energy and the development of nanoscale electronic components and sensing materials. [1] These RCs operate as photodiodes, converting light energy into a linear or cyclic flow of electrons, [2] and at the heart of this biological function lies a light-induced separation of electrical charge. A much commented feature of natural RCs is that they operate with a quantum yield close to unity. [2] In addition to uses in photovoltaic devices [3] and light-powered fuel cells, [4] RCs have potential applications as photosensors, [5] phototransistors, [6] biosensors for detection of environmental pollutants. [7] A variety of photoelectrochemical cells based on RCs have been constructed, [3] mostly comprising two or three electrodes with RCs adhered to a working electrode that is submerged in a buffer solution containing one or more redox mediators. [8][9][10][11] A number of linkers have been employed to achieve oriented adhesion of RCs to the electrode surface, and in many cases construction of the working electrode has required a complex, multistep fabrication procedure. Photocurrent densities between one and several hundred nA cm À2 have typically been obtained in response to red light of an intensity of a few tens of mW cm À2 , [8,9] and a small number of studies have reported higher photocurrents in the mA or even mA range. [9][10][11][12] Herein, protein photoelectrochemical cells were constructed using either RCs or RC-LH1 complexes from Rhodobacter (Rba.) sphaeroides. In the RC-LH1 complex a central RC is surrounded by a light harvesting 1 (LH1) pigment protein (the structures and mechanism of these complexes are described in Figures S1 and S2 in the Support-ing Information). A very simple fabrication procedure was used in which a mixture of protein and the redox mediator N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) was injected into an approximately 10 mL cavity formed between a fluorine-doped tin oxide (FTO) glass front electrode and a Pt-coated rear electrode (see the Supporting Information). The cells had an area of around 1 cm 2 , and the absorbance spectra of the proteins within the cells were consistent with their spectra in detergent solution ( Figure S3 in the Supporting Information). Cells were illuminated with white light passed through a 570 nm long-pass filter.Illumination of a cell that was constructed using a mixture of RC-LH1 complexes and TMPD produced a short-circuit current density (J SC ) that stabilized at a value of 0.15 mA cm À2 after approximately 20 s (Figure 1 a); the direction of the current indicates electron flow into the cell cavity from the FTO-glass front electrode. The initial spike of current flow was similar to a feature that has been seen in photoelectric measurements on RCs in planar lipid bilayers [13] and Langmuir Blodgett films. [14] The initial spike was consistently se...

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Control of protein orientation in molecular photoelectric devices using Langmuir—Blodgett films of photosynthetic reaction centers from Rhodopseudomonas viridis

Cited by 54 publications

References 10 publications

Generation of Alternating Current in Response to Discontinuous Illumination by Photoelectrochemical Cells Based on Photosynthetic Proteins

Generation of Alternating Current in Response to Discontinuous Illumination by Photoelectrochemical Cells Based on Photosynthetic Proteins

Progress and perspectives in exploiting photosynthetic biomolecules for solar energy harnessing

Generation of Alternating Current in Response to Discontinuous Illumination by Photoelectrochemical Cells Based on Photosynthetic Proteins

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