Generation of Alternating Current in Response to Discontinuous Illumination by Photoelectrochemical Cells Based on Photosynthetic Proteins

Tan, Swee Ching; Crouch, Lucy I.; Jones, Michael R.; Welland, Mark E.

doi:10.1002/ange.201200466

Cited by 16 publications

(43 citation statements)

References 50 publications

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“…2b , black). The transient reverse current observed after turning off the excitation light is probably due to dissipation of stored charges in the system, as discussed previously 16 45 . Measurements with monochromatic excitation showed that the current amplitude tracked the absorbance spectrum of the RC in the near infrared ( Fig.…”

Section: Resultssupporting

confidence: 61%

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Demonstration of asymmetric electron conduction in pseudosymmetrical photosynthetic reaction centre proteins in an electrical circuit

Kamran

Friebe²,

Delgado³

et al. 2015

Nat Commun

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Photosynthetic reaction centres show promise for biomolecular electronics as nanoscale solar-powered batteries and molecular diodes that are amenable to atomic-level re-engineering. In this work the mechanism of electron conduction across the highly tractable Rhodobacter sphaeroides reaction centre is characterized by conductive atomic force microscopy. We find, using engineered proteins of known structure, that only one of the two cofactor wires connecting the positive and negative termini of this reaction centre is capable of conducting unidirectional current under a suitably oriented bias, irrespective of the magnitude of the bias or the applied force at the tunnelling junction. This behaviour, strong functional asymmetry in a largely symmetrical protein-cofactor matrix, recapitulates the strong functional asymmetry characteristic of natural photochemical charge separation, but it is surprising given that the stimulus for electron flow is simply an externally applied bias. Reasons for the electrical resistance displayed by the so-called B-wire of cofactors are explored.

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Section: Resultssupporting

confidence: 61%

“…The particular Rba. sphaeroides RC used in the present work has been efficiently interfaced with unfunctionalized gold electrodes 15 , used to generate direct or alternating photocurrents 15 16 and in addition to photovoltaics per se it has been used for applications such as biosensing 17 .…”

mentioning

confidence: 99%

Demonstration of asymmetric electron conduction in pseudosymmetrical photosynthetic reaction centre proteins in an electrical circuit

Kamran

Friebe²,

Delgado³

et al. 2015

Nat Commun

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“…There is a large interest in substituting natural redox carriers (cytochrome and quinones) by inorganic carrier matrices, which are able to donate/accept electrons to/from the RC as secondary electron donors/acceptors. These functional redox active bionanohybrid materials are promising models for photoelectric energy conversion in either optoelectronic or photovoltaic devices (for reference see, e.g., [59][60][61][62], details are given in the forthcoming paragraphs).…”

Section: Photosynthetic Systems In Bionanotechnologymentioning

confidence: 99%

Functional Nanohybrid Materials from Photosynthetic Reaction Center Proteins

Hajdu

Szabó

Sarrai

et al. 2017

International Journal of Photoenergy

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Application of technical developments in biology and vice versa or biological samples in technology led to the development of new types of functional, so-called "biohybrid" materials. These types of materials can be created at any level of the biological organization from molecules through tissues and organs to the individuals. Macromolecules and/or molecular complexes, membranes in biology, are inherently good representatives of nanosystems since they fall in the range usually called "nano." Nanohybrid materials provide the possibility to create functional bionanohybrid complexes which also led to new discipline called "nanobionics" in the literature and are considered as materials for the future. In this publication, the special characteristics of photosynthetic reaction center proteins, which are "nature's solar batteries," will be discussed in terms of their possible applications for creating functional molecular biohybrid materials.

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“…1a, and see Fig. S1 in Supporting information) facilitates a highly-efficient photochemical charge separation that has been exploited for the construction of prototype solar cells [5], [6], [7], photoelectrochemical cells [8], [9], [10], [11], [12], [13], [14], [15], [16], [17], [18] and biosensors [19], [20]. Reaction centres from oxygenic phototrophs are of particular interest with regard to solar fuel synthesis through water splitting [21], [22] and the powering of catalysis by other redox proteins in non-native, hybrid systems [23], [24].…”

Section: Introductionmentioning

confidence: 99%

“…sphaeroides reaction centres incorporated into photoelectrochemical cells [5], [6], [7], [10], [18], [19]. Deposition of this protein on electrode surfaces in such systems is usually achieved by drop-casting or binding from solution, processes which give limited control over protein orientation at the electrode surface and even less control over packing of protein on the electrode surface.…”

Section: Introductionmentioning

confidence: 99%

Directed assembly of defined oligomeric photosynthetic reaction centres through adaptation with programmable extra-membrane coiled-coil interfaces

Swainsbury

Harniman

Bartolo

et al. 2016

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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A challenge associated with the utilisation of bioenergetic proteins in new, synthetic energy transducing systems is achieving efficient and predictable self-assembly of individual components, both natural and man-made, into a functioning macromolecular system. Despite progress with water-soluble proteins, the challenge of programming self-assembly of integral membrane proteins into non-native macromolecular architectures remains largely unexplored. In this work it is shown that the assembly of dimers, trimers or tetramers of the naturally monomeric purple bacterial reaction centre can be directed by augmentation with an α-helical peptide that self-associates into extra-membrane coiled-coil bundle. Despite this induced oligomerisation the assembled reaction centres displayed normal spectroscopic properties, implying preserved structural and functional integrity. Mixing of two reaction centres modified with mutually complementary α-helical peptides enabled the assembly of heterodimers in vitro, pointing to a generic strategy for assembling hetero-oligomeric complexes from diverse modified or synthetic components. Addition of two coiled-coil peptides per reaction centre monomer was also tolerated despite the challenge presented to the pigment-protein assembly machinery of introducing multiple self-associating sequences. These findings point to a generalised approach where oligomers or longer range assemblies of multiple light harvesting and/or redox proteins can be constructed in a manner that can be genetically-encoded, enabling the construction of new, designed bioenergetic systems in vivo or in vitro.

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Generation of Alternating Current in Response to Discontinuous Illumination by Photoelectrochemical Cells Based on Photosynthetic Proteins

Cited by 16 publications

References 50 publications

Demonstration of asymmetric electron conduction in pseudosymmetrical photosynthetic reaction centre proteins in an electrical circuit

Demonstration of asymmetric electron conduction in pseudosymmetrical photosynthetic reaction centre proteins in an electrical circuit

Functional Nanohybrid Materials from Photosynthetic Reaction Center Proteins

Directed assembly of defined oligomeric photosynthetic reaction centres through adaptation with programmable extra-membrane coiled-coil interfaces

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