Control of Papaya Ringspot Virus by Cross Protection.

Yeh, Shyi-Dong; Gonsalves, D.; Wang, Hui-Liang; Namba, R.; Chiu, R. J.

doi:10.1094/pd-72-0375

Cited by 115 publications

(38 citation statements)

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“…Yeh et al (1988) premunizaram eficientemente mudas de mamoeiro com uma estirpe fraca do papaya ringspot virus -type P (PRSV-P), aplicando o inóculo com uma pistola para pintura, com pressão de 4-6 kg cm -2 . O inóculo da estirpe fraca foi diluído na proporção de 1 g de tecido para 20 mL de tampão.…”

Section: Resultados E Discussão Proteção Das Estirpes Fracas Em Casa unclassified

Efeitos da premunização da abóbora 'Menina Brasileira' com estirpes fracas do vírus-do-mosaico-do-mamoeiro - estirpe melancia

Rezende

Pacheco

Iemma

1999

Pesq. agropec. bras.

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RESUMO -Neste trabalho avaliou-se o efeito da premunização com duas estirpes fracas do vírus-domosaico-do-mamoeiro -estirpe melancia (papaya ringspot virus -type W, PRSV-W), combinada com a tolerância das plantas, no controle do mosaico e na produtividade da abóbora Menina Brasileira. Testes realizados em casa de vegetação mostraram que as plantas dessa cultivar premunizadas com as estirpes fracas PRSV-W-1 e 2 ficaram totalmente protegidas contra a infecção por uma estirpe severa de Campinas. Em condições de campo avaliou-se comparativamente a produção de plantas premunizadas, infectadas com a estirpe severa de Campinas, e sadias e expostas à infecção natural (controle). A proteção foi medida com base na produção individual das plantas, cujos frutos foram classificados em comerciais e não-comerciais. As plantas premunizadas tiveram uma produção média de frutos comerciais (peso) 33% superior à daquelas naturalmente infectadas em campo. Quanto ao número de frutos comerciais, o aumento foi da ordem de 50%. A premunização combinada com a tolerância da abóbora Menina Brasileira permitiu um melhor controle do mosaico, com ganhos na produção de frutos comerciais.Termos para indexação: Cucurbita moschata, controle de doença. EFFECTS OF CROSS PROTECTION WITH MILD STRAINS OF PRSV-W ON MENINA BRASILEIRA SQUASHABSTRACT -Cross protection with mild strains combined with tolerance was evaluated for control of papaya ringspot virus -type W (PRSV-W) in squash cultivar Menina Brasileira. Tests carried out in the greenhouse showed that plants premunized with PRSV-W-1 and 2 mild strains were protected against infection with a severe strain of the virus. Protection in the field was evaluated comparing plants premunized with the mild strains, plants infected with severe strains and healthy plants exposed to natural infection (control). Protection index was obtained through yield of marketable and non marketable fruits harvested from individual plants. Average yield (weight) of marketable fruits from protected plants was approximately 33% higher than that of naturally infected plants in the field. Combination of mild strain protection and tolerance of Menina Brasileira provided a better disease control with a significant increase of fruit yield.

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Section: Resultados E Discussão Proteção Das Estirpes Fracas Em Casa unclassified

Efeitos da premunização da abóbora 'Menina Brasileira' com estirpes fracas do vírus-do-mosaico-do-mamoeiro - estirpe melancia

Rezende

Pacheco

Iemma

1999

Pesq. agropec. bras.

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“…An attenuated strain (ZYMV-2002) of Zucchini yellow mosaic virus (ZYMV) isolated through low temperature treatment has also been applied to cucumber (Kosaka et al 2006). Papaya in Hawaii and Taiwan was tested for cross protection by an attenuated strain of PRSV (HA-21), showing significant protection (Yeh et al 1988). Some attenuated, field-selected strains of Cacao swollen shoot virus (CSSV) have been used for cross protection on cacao to prevent severe damage from CSSV (Hughes and Ollennu 1994).…”

Section: Selection Isolation and Induction Of Attenuated Virusesmentioning

confidence: 99%

Attenuated plant viruses: preventing virus diseases and understanding the molecular mechanism

Nishiguchi

Kobayashi

2011

J Gen Plant Pathol

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Attenuated viruses have been isolated and studied not only as a practical means of controlling virus diseases but also to gain a molecular understanding of viral virulence and cross protection. They have been isolated from crop fields and generated through high/low temperature treatment or by mutagens such as nitrous acid and ultraviolet irradiation. Some viruses have been beneficially used in fields and evaluated for one or more decades. Molecular genetic studies on attenuated viruses have revealed that amino acid substitutions are located in replicase and the movement protein in tobamovirus, protein 2b for cucumovirus, and P1 and HC-Pro for potyvirus. In most cases, with a few exceptions, symptom attenuation is positively correlated with a reduced level of RNA silencing suppression. Molecular mechanisms underlying virus attenuation and cross protection and the rationale for practical use of attenuated viruses for effective virus disease control are discussed.

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“…These plants exhibit ''pathogen-derived resistance'' through a process that might be similar to the natural phenomenon of viral cross protection [68][69][70]. The cp of the PRSV is the dominant viral gene and has been the preferred choice of scientists in 11 countries to develop PRSV-resistant papaya through genetic engineering [11].…”

Section: Development Of Economically Important Traits In Papaya By Gementioning

confidence: 99%

Recent advances in the development of transgenic papaya technology

Mendoza

Laurena

Botella

2008

Biotechnology Annual Review

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Abstract. Papaya with resistance to papaya ringspot virus (PRSV) is the first genetically modified tree and fruit crop and also the first transgenic crop developed by a public institution that has been commercialized. This chapter reviews the different transformation systems used for papaya and recent advances in the use of transgenic technology to introduce important quality and horticultural traits in papaya. These include the development of the following traits in papaya: resistance to PRSV, mites and Phytophthora, delayed ripening trait or long shelf life by inhibiting ethylene production or reducing loss of firmness, and tolerance or resistance to herbicide and aluminum toxicity. The use of papaya to produce vaccine against tuberculosis and cysticercosis, an infectious animal disease, has also been explored. Because of the economic importance of papaya, there are several collaborative and independent efforts to develop PRSV transgenic papaya technology in 14 countries. This chapter further reviews the strategies and constraints in the adoption of the technology and biosafety to the environment and food safety. Constraints to adoption include public perception, strict and expensive regulatory procedures and intellectual property issues.

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Control of Papaya Ringspot Virus by Cross Protection.

Cited by 115 publications

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Efeitos da premunização da abóbora 'Menina Brasileira' com estirpes fracas do vírus-do-mosaico-do-mamoeiro - estirpe melancia

Efeitos da premunização da abóbora 'Menina Brasileira' com estirpes fracas do vírus-do-mosaico-do-mamoeiro - estirpe melancia

Attenuated plant viruses: preventing virus diseases and understanding the molecular mechanism

Recent advances in the development of transgenic papaya technology

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