Control of neuronal network organization by chemical surface functionalization of multi-walled carbon nanotube arrays

Abstract

Carbon nanotube substrates are promising candidates for biological applications and devices. Interfacing of these carbon nanotubes with neurons can be controlled by chemical modifications. In this study, we investigated how chemical surface functionalization of multi-walled carbon nanotube arrays (MWNT-A) influences neuronal adhesion and network organization. Functionalization of MWNT-A dramatically modifies the length of neurite fascicles, cluster inter-connection success rate, and the percentage of neurites that escape from the clusters. We propose that chemical functionalization represents a method of choice for developing applications in which neuronal patterning on MWNT-A substrates is required.

Bioactive nanocarbon assemblies: Nanoarchitectonics and applications

“…Different neuronal cell functions such as cell adhesion and network organization were observed from different CNTs. The results may be due to the presence of certain functional groups that can adsorb cell regulating molecules and proteins [91].…”

Application of Carbon Nanotubes in Breast Cancer Therapy

“…A MWNTs encompass an exclusive nanomaterial type that is remarkably durable and electrically conductive owing to the high aspect ratio and chemistry of the particles [38,39]. MWNTs are comparatively long, with a cylindrical shaped-nanoparticles whose honeycomb structure produces high surface area relative to their volume, and high porosity.…”

Repairing Peripheral Nerves: Is there a Role for Carbon Nanotubes?

“…In a recent similar study, embryonic hippocampal neurons were grown on a set of MWCNTs (MWCNT‐A 0 ‐A 5 ) chemically functionalised covalently or non‐covalently to assess the effect of functionalisation on neuronal growth . MWCNT‐A 1 ‐A 5 were all prepared from raw MWCNT‐A 0 that was initially coated onto silicon wafer.…”

“…When assessing the suitability of functionalised CNT scaffolds in vitro, careful consideration must be given to control substrates for comparison. Glass coverslips and TCP coated with polyamines such as PDL, PLL, PEI and poly ornithine have been extensively used as control substrates when neurite outgrowth has been assessed on CNTs . Polyamines are commonly used for coating culture surfaces to enhance cellular adhesion through interactions with negatively charged groups on cell surfaces .…”