Control of microorganisms of oral health interest with Arctium lappa L. (burdock) extract non-cytotoxic to cell culture of macrophages (RAW 264.7)

Oliveira, Jonatas Rafael de; Almeida, Rosilene Batista de Aguiar; Vilela, Polyana das Graças Figueiredo; Oliveira, Felipe Eduardo de; Rocha, Rosilene Fernandes da; Jorge, Antônio Olavo Cardoso; Oliveira, Luciane Dias de

doi:10.1016/j.archoralbio.2014.05.013

Cited by 26 publications

(17 citation statements)

References 30 publications

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“…The herbal extracts of E. arvense L. possess antimicrobial, anti-inflammatory and anticancer effects [24][25][26][27][28][29][30] . The essential oil of E. arvense exerted antibacterial and anticandidal potentials [31][32][33] . To date, 25 compounds with antimicrobial activities have been identified in essential oil obtained from the aerial parts of the plant 25,35 .…”

Section: Introductionmentioning

confidence: 99%

Anticandidal effect of endophytic bacteria isolated from Equisetum arvense L. against Candida albicans and Candida glabrata

Das

Patra

Choi

et al. 2017

Braz. arch. biol. technol.

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Section: Introductionmentioning

confidence: 99%

Anticandidal effect of endophytic bacteria isolated from Equisetum arvense L. against Candida albicans and Candida glabrata

Das

Patra

Choi

et al. 2017

Braz. arch. biol. technol.

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“…Absorbance was read using a microplate reader (Biochrom ® , EZ Reader 400 Microplate Reader) at a wavelength of 570 nm. 14…”

Section: Metabolic Activitymentioning

confidence: 99%

Antifungal, antibiofilm, and antiproliferative activities of Guapira graciliflora Mart

et al. 2018

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The aim of this study was to evaluate in vitro the antifungal, antibiofilm and antiproliferative activities of the extract from the leaves of Guapira graciliflora Mart. The phytochemical characterization of the extract was performed using electrospray ionization mass spectrometry (ESI-MS). The antimicrobial activity of the extract and its fractions was evaluated using the broth microdilution method against species of Candida. The inhibition of C. albicans biofilm was evaluated based on the number of colony-forming units (CFU) and metabolic activity (MTT). The antiproliferative activity of the extract and its fraction was evaluated in the presence of human tumor and non-tumor cells, and the cytotoxicity of the extract was determined on the RAW 264.7 macrophage line - both using the sulforhodamine B method. The phytochemical characterization indicated the presence of the flavonoids rutin and kaempferol. The extract and the methanol fraction exhibited moderate antifungal activity against C. albicans, C. krusei, and C. glabrata, and strong activity against C. dubliniensis. In the biofilms at 24 and 48 hours, the concentration of 12500 µg/mL of the extract was the most effective at reducing the number of CFU s/mL (44.4% and 42.9%, respectively) and the metabolic activity of C. albicans cells (34.6% and 52%, respectively). The extract and its fractions had no antiproliferative effect on the tumor lines tested, with mean activity (log GI50) equal to or greater than 1.71 µg/mL. Macrophage cell viability remained higher than 80% for concentrations of the extract of up to 62.5 µg/mL. G. graciliflora has flavonoids in its chemical composition and demonstrates potential antifungal and antibiofilm activity, with no evidence of a significant change in the viability of human tumor and non-tumor cell lines.

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“…These results are in agreement with those of previously published manuscripts [16,24] reporting on the incorporation of different amounts of TiO 2 and evaluation of their antimicrobial properties. Even though the biofilms may be ten to a thousand times more resistant to an antimicrobial agent [48], because the HAp acts as a synergic agent, enhancing the antimicrobial function of TiO 2 .…”

Section: Tablementioning

confidence: 99%

Biomineralized diamond-like carbon films with incorporated titanium dioxide nanoparticles improved bioactivity properties and reduced biofilm formation

Lopes

Oliveira

Milani

et al. 2017

Materials Science and Engineering: C

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Recently, the development of coatings to protect biomedical alloys from oxidation, passivation and to reduce the ability for a bacterial biofilm to form after implantation has emerged. Diamond-like carbon films are commonly used for implanted medical due to their physical and chemical characteristics, showing good interactions with the biological environment. However, these properties can be significantly improved when titanium dioxide nanoparticles are included, especially to enhance the bactericidal properties of the films. So far, the deposition of hydroxyapatite on the film surface has been studied in order to improve biocompatibility and bioactive behavior. Herein, we developed a new route to obtain a homogeneous and crystalline apatite coating on diamond-like carbon films grown on 304 biomedical stainless steel and evaluated its antibacterial effect. For this purpose, films containing two different concentrations of titanium dioxide (0.1 and 0.3g/L) were obtained by chemical vapor deposition. To obtain the apatite layer, the samples were soaked in simulated body fluid solution for up to 21days. The antibacterial activity of the films was evaluated by bacterial eradication tests using Staphylococcus aureus biofilm. Scanning electron microscopy, X-ray diffraction, Raman scattering spectroscopy, and goniometry showed that homogeneous, crystalline, and hydrophilic apatite films were formed independently of the titanium dioxide concentration. Interestingly, the diamond-like films containing titanium dioxide and hydroxyapatite reduced the biofilm formation compared to controls. A synergism between hydroxyapatite and titanium dioxide that provided an antimicrobial effect against opportunistic pathogens was clearly observed.

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Control of microorganisms of oral health interest with Arctium lappa L. (burdock) extract non-cytotoxic to cell culture of macrophages (RAW 264.7)

Cited by 26 publications

References 30 publications

Anticandidal effect of endophytic bacteria isolated from Equisetum arvense L. against Candida albicans and Candida glabrata

Anticandidal effect of endophytic bacteria isolated from Equisetum arvense L. against Candida albicans and Candida glabrata

Antifungal, antibiofilm, and antiproliferative activities of Guapira graciliflora Mart

Biomineralized diamond-like carbon films with incorporated titanium dioxide nanoparticles improved bioactivity properties and reduced biofilm formation

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