Control of Marek's Disease in the Netherlands. I. Isolation of an Avirulent Marek's Disease Virus (Strain CVI 988) and Its Use in Laboratory Vaccination Trials

Bh, Rispens; H, van Vloten; Mastenbroek, N; Hj, Maas; Ka, Schat

doi:10.2307/1588905

Cited by 301 publications

(142 citation statements)

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“…FC126 strain of HVT (Witter et al, 1970) and CVI988 strain of MDV (Rispens et al, 1972) were obtained from a commercial source and also used as vaccines in one experiment. All the vaccines were of cell-associated type, and plaque-forming-units (PFU) per dose were 1700 PFU for H2 strain, 2740 PFU for FC126 strain and 660 PFU for CVI988 strain.…”

Section: Virusesmentioning

confidence: 99%

Depression of vaccinal immunity to marek's disease by infection with chicken Anaemia agent

et al. 1988

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Section: Virusesmentioning

confidence: 99%

Depression of vaccinal immunity to marek's disease by infection with chicken Anaemia agent

et al. 1988

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“…Attenuated and very virulent MDV serotype 1 (MDV1), CVI988 [30] (Kyoritsushoji Co., Ltd., Tokyo, Japan) and Md5 [42], respectively, and non-pathogenic MDV serotype 2 (MDV2), SB-1 [33], were propagated in CEF or chicken kidney cells (CKC). CEF and CKC were grown in modified Eagle's medium containing 0.3% tryptose phosphate broth (TPB/MEM), supplemented with 10% (growth medium) or 1% calf serum (maintenance medium).…”

Section: Methodsmentioning

confidence: 99%

Heparin Inhibits Plaque Formation by Cell-free Marek's Disease Viruses in vitro.

Lee

Ohashi

Sugimoto

et al. 2001

The Journal of Veterinary Medical Science

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ABSTRACT. The mechanisms of Marek's disease virus (MDV) entry to host cells have not yet been analyzed. Heparan sulfate (HS) on the cell surface serves as a receptor for several herpesviruses in mammalian species. In this study, we demonstrated that plaque formation by cell-free MDV is inhibited by the addition of soluble heparin to the cell culture. Moreover, pretreatment of susceptible cells, chicken embryo fibroblasts, with heparinase, partially reduced infectivity of the cell-free MDV. From these results, it was suggested that the MDV entry, at least in the case of cell-free MDV, is dependent on the presence of cell surface glycosaminoglycans, principally HS. KEY WORDS: cell-free MDV, heparan sulfate, heparin.J. Vet. Med. Sci. 63(4): 427-432, 2001 The primary event of any viral infection is attachment of virus to its host cell. Viral receptors are often involved in defining the host range and specific tissue tropism of a virus. Attachment of herpesviruses to target cells is mediated by viral glycoproteins which are embedded in the virion envelope, and which interact with cellular surface components acting as virus receptors. The first virus found to bind to heparan sulfate (HS) was herpes simplex virus (HSV) [43], and since then, a number of herpesviruses have been demonstrated to use HS as an initial receptor for their entry. In the cases of the alphaherpesviruses, initial interaction between the virion and the target cell involves binding of glycoprotein C (gC) [37] and/or glycoprotein B (gB) [22] to cell-surface glycosaminoglycans (GAG), in particular, HS, which are components of proteoglycans. This interaction with HS has been observed for HSV-1, and HSV-2 [43], pseudorabies virus (PRV) [24], varicella-zoster virus [44], and bovine herpesvirus 1 (BHV-1) [27]. In addition, the gamma- [40] and betaherpesviruses [10,35] Marek's disease virus (MDV), an avian herpesvirus, is the causative agent of Marek's disease (MD), which is characterized by CD4 + T cell lymphoma and nerve enlargement caused by infiltration of lymphocytes and/or lymphoma cells. The pathogenesis of MD can be sequentially divided into three phases: early cytolytic infection; latent infection; and secondary cytolytic infection with immunosuppression and tumor development [26,32,34]. Early cytolytic infection occurs mainly in B cells, and latently infected lymphocytes are mainly activated T cells [36]. In addition, target cells for transformation by MDV are mainly CD4 + T cells [34], suggesting that latent infection in the T cell subsets could be intimately related to the subsequent transformation by MDV. MDVs are highly cell-associated, and the cellfree virus particles are produced only in feather follicle epithelium (FFE) of MDV-infected chickens [6]. However, the mechanism(s) by which MDV attaches to its host cells has not been delineated.Here, we demonstrated that, cell-free MDVs, experimentally prepared from cell culture in vitro, were infectious to cells not only in vitro but also in vivo. In addition, we also showed that cell surfac...

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“…The chicks were inoculated intra-abdominally at 1 day of age with 0.5 ml of a suspension containing 10 4 PFU per ml of the virulent strain K MDV (Rispens et al, 1972); virus isolation and preparation of the suspension have been described by Maas et aL (1981). The spreader chicks, hatched 3 weeks prior to the test chicks, were placed in the rearing house immediately after inoculation.…”

Section: Exposure To MDVmentioning

confidence: 99%

Evaluation of resistance to Marek's disease in three white Plymouth rock unes and in two reciprocal crosses

Jh³

et al. 1982

Avian Pathology

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SUMMARYTwo White Plymouth Rock lines (WPR), both developed into lines resistant for Marek's disease (MD), and one commercial WPR line, supposedly susceptible to the disease, were mated in a number of combinations to evaluate resistance to MD in their pure-and crossbred progeny. For this purpose the pedigreed 1-day-old progeny chicks were exposed to 3-week-old spreader chicks which had been inoculated at 1-day-old with virulent MD virus (MDV) and reared with them for the whole experimental period of 15 weeks.Response to the challenge showed that resistance to MD in the crossbreds was intermediate to that of resistant and susceptible parental Unes. It appeared that, besides a sex effect in the male progeny, the trait for MD resistance was most effectively transferred to the crossbred progeny by the resistant male parents.

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Control of Marek's Disease in the Netherlands. I. Isolation of an Avirulent Marek's Disease Virus (Strain CVI 988) and Its Use in Laboratory Vaccination Trials

Cited by 301 publications

References 9 publications

Depression of vaccinal immunity to marek's disease by infection with chicken Anaemia agent

Depression of vaccinal immunity to marek's disease by infection with chicken Anaemia agent

Heparin Inhibits Plaque Formation by Cell-free Marek's Disease Viruses in vitro.

Evaluation of resistance to Marek's disease in three white Plymouth rock unes and in two reciprocal crosses

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