Control of cell-contact sites by cyclic AMP pulses in differentiating Dictyostelium cells

Gerisch, Günther; Fromm, Hillel; Huesgen, Adolfine; Wick, U.

doi:10.1038/255547a0

Cited by 318 publications

(99 citation statements)

References 16 publications

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“…Which form of phosphodiesterase plays the most important role in controlling the cGMP content in intact cells is not known. These results indicate a role of cGMP during chemotaxis in D. discoideum, cAMP and frolic acid not only induce chemotaxis [2,3] but also cell differentiation [25][26][27] and, in the case of cAMP, adenylate cyclase activation [28]. More information is required to know which of these biological effects are regulated by cGMP.…”

Section: Discussionmentioning

confidence: 99%

Cyclic AMP and folic acid mediated cyclic GMP accumulation in Dictyostelium discoideum

et al. 1977

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Section: Discussionmentioning

confidence: 99%

Cyclic AMP and folic acid mediated cyclic GMP accumulation in Dictyostelium discoideum

et al. 1977

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“…One of the consequences of pulsatile signalling in starving amoebae is the formation of glycoprotein 'contact sites A' that are concerned with the adhesion of cells towards the end of the aggregation phase (Beug et al, 1973;Gerisch et al, 1975). It has been found with aggregation-defective mutants that the inability to produce or relay pulsatile cyclic AMP signals inhibits formation of contact sites A, unless exogenous cyclic AMP pulses are provided (Darmon et al, 1977;Glazer & Newell, 1981).…”

Section: Mechanism Of Adenosine Inhibition Of Centre Initiationmentioning

confidence: 99%

Inhibition by Adenosine of Aggregation Centre Initiation and Cyclic AMP Binding in Dictyostelium

Newell

Ross

1982

Microbiology

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The inhibitory action of adenosine on the initiation of aggregation centre formation in starving Dictyostelium discoidem was examined and used to test assumptions of theoretical models of cyclic AMP signalling. Adenosine at 5 mM strongly inhibited aggregation centre initiation without preventing signal relay. Inosine or AMP at 10 mM had no effect and did not prevent the effect of adenosine. Adenosine had no effect on cellular phosphodiesterase activity, contact site A formation, or chemotaxis to small drops of cyclic AMP. The chemotactically-related responses of rapid cyclic GMP formation and changes in light scattering after pulses of cyclic AMP did not show any inhibition or decrease in sensitivity to the signal in the presence of adenosine. However, binding of 3H-labelled cyclic AMP to cell surface receptors was strongly inhibited by adenosine. The maximum inhibition was approximately 90% and was non-competitive with respect to cyclic AMP. The role of cell surface cyclic AMP receptors in the mechanism of initiation and the implications of the effects of adenosine for the role of cyclic GMP as a secondary messenger are discussed.

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“…The affected cells secrete cAMP in response to the original pulse, resulting in signal amplification and relay and giving rise to periodic outward propagating waves of cAMP that direct cell movement toward the origin (4)(5)(6)(17)(18)(19)(20). Another role for cAMP early in development has been revealed by observations that either pulses or high continuous levels of exogenous cAMP induce prematurely the appearance of several developmentally regulated proteins (21)(22)(23)(24)(25)(26)(27)(28). High concentrations of cAMP can also induce formation of stalk (29,30) and spore (30) (35)(36)(37) suggest that tips are centers for the synthesis of cAMP.…”

mentioning

confidence: 99%

A role for cyclic AMP in expression of developmentally regulated genes in Dictyostelium discoideum.

Landfear¹,

Lodish²

1980

Proc. Natl. Acad. Sci. U.S.A.

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Starved cells of Dictyostelium discoideum begin to synthesize a new class of developmentally regulated proteins at about 13 hr of the 24-hr developmental program, concomitant with the formation of tips on the tight cell aggregates [Alton, Dev. Biol. 60, 180-2061. Continued synthesis of these proteins is normally dependent upon the integrity of the multicellular aggregates, because cells that have been disaggregated at 13 hr and shaken in suspension for 5 hr do not make these proteins. We show here that addition of 20 AM cyclic AMP to suspension cultures of disaggregated 13-hr cells caused synthesis of most of these late proteins to be maintained. Translation in an in vitro wheat germ system of total cellular RNA isolated from these cyclic AMP-stimulated suspension cells, or from normal aggregates, generated several proteins that were not encoded by the RNA isolated from equivalent suspension cells which had not been treated with cyclic AMP or from preaggregation cells. We conclude that cyclic AMP has a direct role in maintaining the synthesis of aggregation-dependent Dictyostelium proteins and in maintaining the level of the corresponding mRNAs.The cellular slime mold Dictyostelium discoideum presents an ideal eukaryotic system in which to study the control of developmentally regulated genes (1, 2). Growing cells exist as unicellular amoebae in the presence of a complete nutrient medium. When amoebae are starved and plated at an air-liquid interface, a well-defined developmental program ensues in which cells secrete 3',5'-cyclic AMP (cAMP) (3) in a pulsatile manner (4-6), stream toward aggregation centers, and form multicellular aggregates that differentiate into stalk and spore tissues (1, 2). Some genes are expressed only during discrete stages of this developmental cycle: if cells are labeled with 15-to 30-min pulses of [a5S]methionine at various intervals throughout the 24-hr developmental program, and the protein products are separated on O'Farrell two-dimensional gels (7), about 100 of the 400 observable spots change substantially in relative intensity at some period of development (8). The most pronounced alteration occurs around 10-13 hr in the developmental cycle, at the time when tight cell-cell aggregates have formed and tips begin to appear at the top of the mounds: approximately 40 proteins that were made at low levels or not at all in 3-hr cells are induced to high levels of synthesis. The continued pronounced synthesis of these developmentally controlled proteins is dependent upon the maintainance of cell contact, because mounds that are disaggregated selectively arrest or attentuate the synthesis of these late species (9). In this paper, we present evidence that cAMP is directly involved in controlling the expression of this class of genes.The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "ad- Autoradiograms were made by exposing a gel containing 106 cpm of trichloroacetic acid-precipitable -5S for 24 hr; ...

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Control of cell-contact sites by cyclic AMP pulses in differentiating Dictyostelium cells

Cited by 318 publications

References 16 publications

Cyclic AMP and folic acid mediated cyclic GMP accumulation in Dictyostelium discoideum

Cyclic AMP and folic acid mediated cyclic GMP accumulation in Dictyostelium discoideum

Inhibition by Adenosine of Aggregation Centre Initiation and Cyclic AMP Binding in Dictyostelium

A role for cyclic AMP in expression of developmentally regulated genes in Dictyostelium discoideum.

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