Inhibition by Adenosine of Aggregation Centre Initiation and Cyclic AMP Binding in Dictyostelium

Newell, Peter C.; Ross, Fiona

doi:10.1099/00221287-128-11-2715

Cited by 23 publications

(23 citation statements)

References 26 publications

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“…IPA belongs to the group of adenosine derivatives that have been shown to affect binding of cAMP to the cAMP-specific surface receptors [18]. However, we have found that the course of aggregation in the presence of IPA differs considerably from that observed in the presence of adenosine [19]. Although the cells on IPA agar relay the cAMP signal, and optical density waves can be observed in dark-field as in the case of adenosine [19], the cells never form any characteristic streaming patterns due to a concerted cell movement.…”

Section: Introductionmentioning

confidence: 46%

“…However, we have found that the course of aggregation in the presence of IPA differs considerably from that observed in the presence of adenosine [19]. Although the cells on IPA agar relay the cAMP signal, and optical density waves can be observed in dark-field as in the case of adenosine [19], the cells never form any characteristic streaming patterns due to a concerted cell movement. Instead, the waves vanish after a certain time leaving the cell layer in a homogeneous state.…”

Section: Introductionmentioning

confidence: 57%

“…Adenosine is a physiological degradation product of cAMP and has been shown to inhibit several cAMPdepending processes by inhibition of cAMP binding [19] and postaggregative differentiation [18]. Adenosine reduces oscillatory cAMP activity [26,19,27] and plays a role in cell type regulation at later states of the developmental cycle [28,29].…”

Section: Discussionmentioning

confidence: 99%

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Streamless aggregation of Dictyostelium in the presence of isopropylidenadenosin

Hilgardt

Čejková

Hauser

et al. 2008

Biophysical Chemistry

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Section: Introductionmentioning

confidence: 46%

Section: Introductionmentioning

confidence: 57%

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Streamless aggregation of Dictyostelium in the presence of isopropylidenadenosin

Hilgardt

Čejková

Hauser

et al. 2008

Biophysical Chemistry

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“…In wild-type cells, cAMP induction of prespore gene expression is inhibited by millimolar concentrations of adenosine and by micromolar concentrations of the adenosine analogs NECA and IPA, which cannot be phosphorylated by an extracellular adenosine kinase (37,42,43). Adenosine also inhibits cAMP binding (24,41), and recent studies showed that only binding of cAMP to cAR1, but not to cAR2 or cAR3, is inhibited by adenosine (17a). To identify the cAR that mediates prespore gene induction and to verify that the inhibitory effects of adenosine are due to inhibition of cAMP binding FIG.…”

Section: Resultsmentioning

confidence: 99%

Functional Promiscuity of Gene Regulation by Serpentine Receptors in Dictyostelium discoideum

Wijk

Kim

Brandt

et al. 1998

Molecular and Cellular Biology

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Serpentine receptors such as smoothened and frizzled play important roles in cell fate determination during animal development. In Dictyostelium discoideum, four serpentine cyclic AMP (cAMP) receptors (cARs) regulate expression of multiple classes of developmental genes. To understand their function, it is essential to know whether each cAR is coupled to a specific gene regulatory pathway or whether specificity results from the different developmental regulation of individual cARs. To distinguish between these possibilities, we measured gene induction in car1 car3 double mutant cell lines that express equal levels of either cAR1, cAR2, or cAR3 under a constitutive promoter. We found that all cARs efficiently mediate both aggregative gene induction by cAMP pulses and induction of postaggregative and prespore genes by persistent cAMP stimulation. Two exceptions to this functional promiscuity were observed. (i) Only cAR1 can mediate adenosine inhibition of cAMP-induced prespore gene expression, a phenomenon that was found earlier in wild-type cells. cAR1's mediation of adenosine inhibition suggests that cAR1 normally mediates prespore gene induction. (ii) Only cAR2 allows entry into the prestalk pathway. Prestalk gene expression is induced by differentiation-inducing factor (DIF) but only after cells have been prestimulated with cAMP. We found that DIF-induced prestalk gene expression is 10 times higher in constitutive cAR2 expressors than in constitutive cAR1 or cAR3 expressors (which still have endogenous cAR2), suggesting that cAR2 mediates induction of DIF competence. Since in wild-type slugs cAR2 is expressed only in anterior cells, this could explain the so far puzzling observations that prestalk cells differentiate at the anterior region but that DIF levels are actually higher at the posterior region. After the initial induction of DIF competence, cAMP becomes a repressor of prestalk gene expression. This function can again be mediated by cAR1, cAR2, and cAR3.

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“…In Dictyostelium, cyclic AMP acts as a chemoattractant, a morphogen, and a mediator of gene regulation (Bonner, 1970;Konijn et al, 1968;Lodish et al, 1982). It has been suggested that the product of 5NT activity, adenosine, is involved in the regulation of development (Newell and Ross, 1982;Schaap and Wang, 1986;Spek et al, 1988;Theibert and Devreotes, 1984;. It is particularly interesting that a phosphatase activity becomes localized to a narrow region of cells at the interface of the prestalk-prespore boundary during development (Aramaki et al, 1980;Armant and Rutherford, 1979;Krivanek, 1956).…”

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confidence: 98%

Analysis of 5′ nucleotidase and alkaline phosphatase by gene disruption in Dictyostelium

Rutherford

Overall

Ubeidat

et al. 2003

Genesis

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In Dictyostelium discoideum a phosphatase with a high pH optimum is known to increase in activity during cell differentiation and become localized to a narrow band of cells at the interface of prespore and prestalk cells. However, it was not clear if this activity is due to a classical "alkaline phosphatase" with broad range substrate specificity or to a "5'nucleotidase" with high substrate preference for 5'AMP. We attempted to disrupt the genes encoding these two phosphatase activities in order to determine if the activity that is localized to the interface region resides in either of these two proteins. During aggregation of 5nt null mutants, multiple tips formed rather than the normal single tip for each aggregate. In situ phosphatase activity assays showed that the wt and the 5nt gene disruption clones had normal phosphatase activity in the area between prestalk and prespore cell types, while the alp null mutants did not have activity in this cellular region. Thus, the phosphatase activity that becomes localized to the interface of the prestalk and prespore cells is alkaline phosphatase.

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Inhibition by Adenosine of Aggregation Centre Initiation and Cyclic AMP Binding in Dictyostelium

Cited by 23 publications

References 26 publications

Streamless aggregation of Dictyostelium in the presence of isopropylidenadenosin

Streamless aggregation of Dictyostelium in the presence of isopropylidenadenosin

Functional Promiscuity of Gene Regulation by Serpentine Receptors in Dictyostelium discoideum

Analysis of 5′ nucleotidase and alkaline phosphatase by gene disruption in Dictyostelium

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