Control of cell adhesion and spreading by spatial microarranged PEO-like and pdAA domains

Surface Functionalization and Patterning Techniques to Design Interfaces for Biomedical and Biosensor Applications

“…This is illustrated in Figure 4, where cells cover only a masked part of the Petri dishes that had been covered by the 1 W PEO‐like films. In contrast, coatings prepared at higher than 1 W exhibited better cell adhesion properties with a percentage of adhering cells similar to the control in the case of films deposited with RF power higher than 5 W. This results are in agreement with published works where it has shown that a percentage larger than 70% of PEO retention is required for obtaining a repellent effect toward cell attachment 29,48…”

Plasma‐Aided Micro‐ and Nanopatterning Processes for Biomedical Applications

“…The behavior of cells, in this case, is driven by gradients/contrasts of different chemical functionalities on the substrate. The following sequence of steps is a procedure59–61 that has been applied to transfer micro‐arrangements of cell‐adhesive and cell‐repulsive domains on surfaces on polymeric substrates: plasma‐treatment or PE‐CVD process that aims to obtain a homogeneous cell‐adhesive surface all over the substrate; positioning of the physical mask in close contact with the substrate; plasma treatment or PE‐CVD process that aims to obtain cell‐repulsive surfaces, performed through the physical mask. …”

“… 1) Plasma modification of the substrate, 2) plasma patterning through TEM grids60 and 3) through nanocolloidal latex beads85,86 masks to obtain, respectively, micro‐ and nanosized features. A plasma process can be performed before the patterning procedure to obtain chemically different domains or to improve the self assembly of the colloidal particles.…”

Trends in surface engineering of biomaterials: atmospheric pressure plasma deposition of coatings for biomedical applications

“…High versatility, minimum consumption of reagents, no use of solvents, high/easy integration in existing industrial processes are other advantages of the technique. In surface engineering of biomaterials and devices low pressure (LP) have a longer tradition than atmospheric pressure (AP) plasma processes [2][3][4][5][6][7][8][9]. However, also AP plasmas are being investigated for surface engineering of biomaterials, as we will show in the text, but they entered recently in this field.…”

“…AP Plasma Liquid Deposition (AP-PLD) and AP Plasma Enhanced Chemical Vapours Depositions (AP-PECVD). LP Plasma deposited PEO-like coatings are known for their non fouling properties in water media against protein, cells and bacteria [4,38,39]. In the AP-PLD process, liquid CH 3 O(CH 2 CH 2 O) 4 CH 3 (TEGDME) was sprayed on the substrate and then exposed to an Ar AP plasma torch.…”