Control of Boophilus microplus populations in grazing cattle vaccinated with a recombinant Bm86 antigen preparation

Rodrı́guez, Manuel; Penichet, Manuel L.; Mouris, A.E.; Labarta, Violeta; Luaces, L. Lorenzo; Rubiera, Roger; Cordovés, C. O.; Sánchez, Pedro; Ramos, Eduardo Antônio Gonçalves; Hernández-Soto, Alejandro; Canales, Mario; Palenzuela, Daniel; Triguero, Ada; Lleonart, Ricardo; Herrera, Luís; Fuente, José de la

doi:10.1016/0304-4017(94)00678-6

Cited by 91 publications

(55 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Induction of tolerance toward otherwise protective epitopes could result in increased infection levels. In this case, the immunization schedule, including dose, route, adjuvant used and frequency of immunization, was similar to previous studies using recombinant proteins [33–35]. Additionally induction of tolerance generally requires large amounts (6mg or more) of antigen [36, 37].…”

Section: Discussionmentioning

confidence: 57%

Subdominant Outer Membrane Antigens in Anaplasma marginale: Conservation, Antigenicity, and Protective Capacity Using Recombinant Protein

et al. 2015

View full text Add to dashboard Cite

Anaplasma marginale is a tick-borne rickettsial pathogen of cattle with a worldwide distribution. Currently a safe and efficacious vaccine is unavailable. Outer membrane protein (OMP) extracts or a defined surface protein complex reproducibly induce protective immunity. However, there are several knowledge gaps limiting progress in vaccine development. First, are these OMPs conserved among the diversity of A. marginale strains circulating in endemic regions? Second, are the most highly conserved outer membrane proteins in the immunogens recognized by immunized and protected animals? Lastly, can this subset of OMPs recognized by antibody from protected vaccinates and conserved among strains recapitulate the protection of outer membrane vaccines? To address the first goal, genes encoding OMPs AM202, AM368, AM854, AM936, AM1041, and AM1096, major subdominant components of the outer membrane, were cloned and sequenced from geographically diverse strains and isolates. AM202, AM936, AM854, and AM1096 share 99.9 to 100% amino acid identity. AM1041 has 97.1 to 100% and AM368 has 98.3 to 99.9% amino acid identity. While all four of the most highly conserved OMPs were recognized by IgG from animals immunized with outer membranes, linked surface protein complexes, or unlinked surface protein complexes and shown to be protected from challenge, the highest titers and consistent recognition among vaccinates were to AM854 and AM936. Consequently, animals were immunized with recombinant AM854 and AM936 and challenged. Recombinant vaccinates and purified outer membrane vaccinates had similar IgG and IgG2 responses to both proteins. However, the recombinant vaccinates developed higher bacteremia after challenge as compared to adjuvant-only controls and outer membrane vaccinates. These results provide the first evidence that vaccination with specific antigens may exacerbate disease. Progressing from the protective capacity of outer membrane formulations to recombinant vaccines requires testing of additional antigens, optimization of the vaccine formulation and a better understanding of the protective immune response.

show abstract

Section: Discussionmentioning

confidence: 57%

Subdominant Outer Membrane Antigens in Anaplasma marginale: Conservation, Antigenicity, and Protective Capacity Using Recombinant Protein

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Tick resistance against vaccine antigens is less likely than resistance against chemical acaricides, because numerous different antibodies are usually generated against multiple antigen epitopes (Hajdusek et al, 2010). The demonstration that recombinant forms of the protein Bm86 confer protection against R. microplus infestations was a major achievement in parasite vaccinology, proving that a vaccine to protect against an ectoparasite is indeed a realistic possibility (Rand et al, 1989;Rodriguez et al, 1994Rodriguez et al, , 1995aRodriguez et al, , 1995b. Indeed, a Bm86-based tick vaccine was the first ectoparasite vaccine available in the market (Rodriguez et al, 1994).…”

Section: Discussionmentioning

confidence: 97%

Immunoprotective potential of a Rhipicephalus (Boophilus) microplus metalloprotease

Ali

Parizi

Guizzo

et al. 2015

Veterinary Parasitology

View full text Add to dashboard Cite

Ticks have serious impacts on animal and human health, causing significant economic losses in cattle breeding. Besides damage due to the hematophagous behavior, they transmit several pathogens. Low cost and environmental safety have made vaccines a promising alternative control method against tick infestation. Metalloproteases (MPs) have been shown to be essential for diverse biological functions in hematophagous organisms, inhibiting blood clotting, degrading extracellular matrix proteins, and inhibiting host tissue repair via anti-angiogenic activity. In this study, we analyzed the immunoprotective potential of a recombinant MP against Rhipicephalus (Boophilus) microplus infestation. First, a cDNA encoding R. microplus amino acids sequence with highly conserved regions of the metzincin (reprolysin) group of MP was identified (BrRm-MP4). After expression and purification, recombinant BrRm-MP4 was used as a vaccinal antigen against R. microplus infestation in cattle (Bos taurus taurus). All vaccinated bovines developed immune response to the antigen, resulting in increased antibody level throughout the immunization protocol. Immunization with rBrRm-MP4 reduced tick feeding success, decreasing the number of engorged females and their reproduction potential, representing a 60% overall protection. These results show that rBrRm-MP4 provides protection against tick infestation, placing it is a potential candidate for an anti-tick vaccine.

show abstract

“…(B.) microplus and breeds of cattle ranged from 51 to 91 % with the same dose of vaccine Floyd et al 1995;Rodriguez et al 1995;de la Fuente et al 2007). In the present vaccination trial using the same dose of vaccine, a 44.5 % efficacy against R.…”

Section: Discussionmentioning

confidence: 97%

Efficacy of rBm86 against Rhipicephalus (Boophilus) microplus (IVRI-I line) and Hyalomma anatolicum anatolicum (IVRI-II line) infestations on bovine calves

et al. 2012

View full text Add to dashboard Cite

With an aim to evaluate the protective potentiality of rBm86 against Rhipicephalus (Boophilus) microplus Indian Veterinary Research Institute (IVRI)-I line and Hyalomma anatolicum anatolicum IVRI-II line infestations on crossbred (Bos indicus × Bos taurus) calves, 20 animals of 3 months of age were randomly divided in to four equal groups and maintained in tick-proof conditions. Animals of groups 1 and 2 were immunized with 2 ml of rBm86 (100 μg)-based vaccine (procured from Revetmex S.A. de C.V, Mexico City, Mexico) thrice at 30 days interval. Animals of groups 3 and 4 were kept as negative control and inoculated with PBS only. Each animal of group 1 and 3 was challenged with 7-day-old 50 unfed adults of H. anatolicum anatolicum (1:1, male and female), and each animal of groups 2 and 4 was challenged with 6-8-day-old R. (B.) microplus larvae obtained from 50 mg of eggs, on 17th day of the last immunization. The efficacy of rBm86 against tick infestations was determined as percentage reduction in number of adults dropped (DT%), engorged body weight (DR%), egg masses (DO%), and immunogen efficacy (E%). The calculated data were 11.8, 10.8, 15.0, and 25.1 %, respectively, for DT, DR, DO, and E% against H. anatolicum anatolicum infestation, while in the case of R. (B.) microplus infestation, the corresponding data were 6.4, 11.24, 40.7, and 44.5 %, respectively. The results indicated partial effectiveness of rBm86 antigen(s) in imparting protection against homologous and heterologous challenge infestations of Indian ticks. The results indicated identification of more effective antigen(s) for the development of vaccine against economically important tick species in India.

show abstract

Control of Boophilus microplus populations in grazing cattle vaccinated with a recombinant Bm86 antigen preparation

Cited by 91 publications

References 8 publications

Subdominant Outer Membrane Antigens in Anaplasma marginale: Conservation, Antigenicity, and Protective Capacity Using Recombinant Protein

Subdominant Outer Membrane Antigens in Anaplasma marginale: Conservation, Antigenicity, and Protective Capacity Using Recombinant Protein

Immunoprotective potential of a Rhipicephalus (Boophilus) microplus metalloprotease

Efficacy of rBm86 against Rhipicephalus (Boophilus) microplus (IVRI-I line) and Hyalomma anatolicum anatolicum (IVRI-II line) infestations on bovine calves

Contact Info

Product

Resources

About