Contributions from metabolomics to fish research

Samuelsson, Linda M.; Larsson, D. G. Joakim

doi:10.1039/b804196b

Cited by 100 publications

(63 citation statements)

References 40 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…61 A metabolômica ambiental envolve o estudo das repostas metabólicas de organismos a fatores bióticos (competição, mutualismo, relação parasita-hospedeiro, planta-herbívoro, entre outros), e abióticos (temperatura, umidade, disponibilidade de nutrientes, exposição a poluentes, etc.). [62][63][64][65][66][67] A aplicação da metabolômica na área ambiental é muito ampla e envolve o estudo de organismos aquáticos, [67][68][69][70] de plantas, 71 e como estes respondem ao estresse oxidativo, 72 estresse abiótico, [73][74][75] fatores bióticos, 76 ou fatores bióticos e abióticos simultaneamente. 77 Também são encontrados trabalhos de exposição de organismos a poluentes, 78 18,86 entre outros.…”

Section: Ambientalunclassified

Metabolômica: Definições, Estado-Da-Arte E Aplicações Representativas

Canuto¹,

Costa²,

Cruz³

et al. 2017

Quim. Nova

View full text Add to dashboard Cite

METABOLOMICS: DEFINITIONS, STATE-OF-THE-ART AND REPRESENTATIVE APPLICATIONS.Metabolomics is an emerging and promising omics approach used to understand biological mechanisms. By untargeted and targeted metabolomics analyses, metabolites are determined in biological samples (fluids, cells, tissues, etc.) by comparison of control groups with altered groups, undergoing different therapies, submitted to differing stress levels, dietary modulation, or promoted by a disease, or specific condition, etc., using sophisticated analytical techniques, and advanced data treatment and statistical analyses. In this review, the concepts involved in metabolomics studies were presented, describing in details all steps involved in the metabolomics workflow, for untargeted and targeted strategies. Finally, the potential of metabolomics is illustrated by applications in representative areas: clinical, environmental, food and nutrition, forensic toxicology, microbiology, parasitology, plants, and sports. Relevant reviews were compiled to characterize each of these areas, and a corresponding application of untargeted and targeted metabolomics were described.Keywords: metabolomics; untargeted metabolomics; targeted metabolomics; metabolomics workflow. INTRODUÇÃOAs ciências ômicas buscam o entendimento do funcionamento celular dos organismos e suas alterações biológicas. Fazem parte deste conjunto de ciências, a genômica (estudo da alteração dos genes), a transcriptômica (estudo das alterações dos transcritos), a proteômica (estudo das alterações das proteínas), e a metabolômica (estudo das alterações dos metabólitos). Metabólitos são produtos intermediários ou finais do metabolismo em uma amostra biológica.1 O conjunto de todos os metabólitos de baixa massa molecular (até 1500 Da), presentes ou alterados em um sistema biológico, é chamado de metaboloma (do inglês, metabolome).2 A pesquisa relacionada a metabólitos vem sendo desenvolvida há décadas, mas em 1999, Nicholson et al. definiram a metabonômica (do inglês, metabonomics), como sendo a medida quantitativa da resposta metabólica de um sistema biológico após estímulos fisiopatológicos ou modificações genéticas.3 Já o termo metabolômica (do inglês, metabolomics) foi introduzido em 2001, por Oliver Fiehn, como sendo a análise abrangente e quantitativa do metaboloma de um sistema biológico. 4 Além disso, a literatura nos apresenta outras denominações envolvendo esse campo da ciência, tais como: perfil metabólico (do inglês, metabolic profiling), descrito como sendo a análise de metabólitos previamente selecionados de rotas bioquímicas específicas, 5 a impressão digital metabólica (do inglês, metabolic fingerprinting), que é definida como sendo "uma classificação de amostras de acordo com sua origem ou sua relevância biológica", 3 e, por último, análise footprinting (ainda sem tradução para o português), para se referir aos metabólitos excretados por uma célula em condições controladas. Observa-se, portanto, uma divergência em relação às terminologias aplicadas às análises metabolômic...

show abstract

Section: Ambientalunclassified

Metabolômica: Definições, Estado-Da-Arte E Aplicações Representativas

Canuto¹,

Costa²,

Cruz³

et al. 2017

Quim. Nova

View full text Add to dashboard Cite

show abstract

“…Therefore, an NMR based metabolomic approach has been successfully applied to evaluate animal homeostasis, including in human beings (Claesson et al 2012;Clayton et al 2006;Fukuda et al 2011;Furusawa et al 2013;Holmes et al 2008;Schlipalius et al 2012). This NMR based metabolomic approach is also valuable in aquatic ecosystems for studying the environmental effects of pharmaceuticals and other chemicals on fishes (Samuelsson et al 2011;Samuelsson et al 2006;Samuelsson & Larsson 2008). Such studies have contributed knowledge of basic physiology and development of fish, disease, water pollution, and other aspects (Bilandzic et al 2011;Dove et al 2012;Picone et al 2011;Southam et al 2008;Southam et al 2011;Wagner et al 2014;Williams et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Non invasive analysis of metabolic changes following nutrient input into diverse fish species, as investigated by metabolic and microbial profiling approaches

Asakura

Sakata

Yoshida

et al. 2014

Preprint

View full text Add to dashboard Cite

An NMR-based metabolomic approach in aquatic ecosystems is valuable for studying the environmental effects of pharmaceuticals and other chemicals on fish. This technique has also contributed to new information in numerous research areas, such as basic physiology and development, disease, and water pollution. We evaluated the microbial diversity in various fish species collected from Japan's coastal waters using next-generation sequencing, followed by evaluation of the effects of feed type on co-metabolic modulations in fishmicrobial symbiotic ecosystems in laboratory-scale experiments. Intestinal bacteria of fish in their natural environment were characterized (using 16S rRNA genes) for trophic level using pyrosequencing and noninvasive sampling procedures developed to study the metabolism of intestinal symbiotic ecosystems in fish reared in their environment. Metabolites in feces were compared, and intestinal contents and feed were annotated based on HSQC and TOCSY using SpinAssign and network analysis. Feces were characterized by species and varied greatly depending on the feeding types. In addition, feces samples demonstrated a response to changes in the time series of feeding. The potential of this approach as a non-invasive inspection technique in aquaculture is suggested. PrePrints ABSTRACT An NMR based metabolomic approach in aquatic ecosystems is valuable for studying the environmental effects of pharmaceuticals and other chemicals on fish. This technique has also contributed to new information in numerous research areas, such as basic physiology and development, disease, and water pollution. We evaluated the microbial diversity in various fish species collected from Japan's coastal waters using next generation sequencing, followed by evaluation of the effects of feed type on co metabolic modulations in fish microbial symbiotic ecosystems in laboratory scale experiments. Intestinal bacteria of fish in their natural environment were characterized (using 16S rRNA genes) for trophic level using pyrosequencing and noninvasive sampling procedures developed to study the metabolism of intestinal symbiotic ecosystems in fish reared in their environment. Metabolites in feces were compared, and intestinal contents and feed were annotated based on HSQC and TOCSY using SpinAssign and network analysis. Feces were characterized by species and varied greatly depending on the feeding types. In addition, feces samples demonstrated a response to changes in the time series of feeding. The potential of this approach as a non invasive inspection technique in aquaculture is suggested.

show abstract

“…Metabolomics aims in identification and quantitation of metabolites smaller than 1kDa in the metabolome [25]. Metabolomics is also considered as the process of detecting and measuring metabolites changes responding to different status, such as diseases, environmental or genetic perturbation etc.…”

Section: Metabolomics Analysismentioning

confidence: 99%

Development and Application of Liquid Chromatography in Life Sciences

Zhu¹,

Chen²

2017

J Chromatogr Sep Tech

View full text Add to dashboard Cite

Liquid chromatographic approaches cover a wide range of applications today. It is closely relevant in scientific studies, which provides a powerful identification of certain components that will be effective for curing specific diseases. Especially important, LC methods can assist us for isolation and purification of therapeutic drugs that benefit for the development of medical industry. As the development of liquid chromatography, the single effective component within a drug can be separated and purified under extreme purity requirements from a biological system with great complexity and abundance. However, improvement of LC methods is still urgently needed for better application of this method to benefit our lives.

show abstract

Contributions from metabolomics to fish research

Cited by 100 publications

References 40 publications

Metabolômica: Definições, Estado-Da-Arte E Aplicações Representativas

Metabolômica: Definições, Estado-Da-Arte E Aplicações Representativas

Non invasive analysis of metabolic changes following nutrient input into diverse fish species, as investigated by metabolic and microbial profiling approaches

Development and Application of Liquid Chromatography in Life Sciences

Contact Info

Product

Resources

About