Contribution of physical methods in decellularization of animal tissues

Rabbani, Mohsen; Zakian, Nasrin; Alimoradi, Nima

doi:10.4103/jmss.jmss_2_20

Cited by 44 publications

(64 citation statements)

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“…In addition to the above classical methods, several novel treatments, such as vacuum-assisted decellularization and apoptosis-assisted decellularization, are recently emerged. Vacuum-assisted decellularization refers to using negative pressure to accelerate the processing of decellularization [ [148] , [149] , [150] , [151] ]. The method is highly effective when combined with physical, chemical or enzyme treatment; however, the prolonged vacuum time may increase the pore size and destruct the collagen fiber bundles, and thus decrease the mechanical strength of specific tissues, such as pericardium [ 152 , 153 ].…”

Section: Classification and Fabrication Of Decm Scaffoldsmentioning

confidence: 99%

Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering

Zhang

Chen

Hong

et al. 2022

Bioactive Materials

333

263

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The application of scaffolding materials is believed to hold enormous potential for tissue regeneration. Despite the widespread application and rapid advance of several tissue-engineered scaffolds such as natural and synthetic polymer-based scaffolds, they have limited repair capacity due to the difficulties in overcoming the immunogenicity, simulating in-vivo microenvironment, and performing mechanical or biochemical properties similar to native organs/tissues. Fortunately, the emergence of decellularized extracellular matrix (dECM) scaffolds provides an attractive way to overcome these hurdles, which mimic an optimal non-immune environment with native three-dimensional structures and various bioactive components. The consequent cell-seeded construct based on dECM scaffolds, especially stem cell-recellularized construct, is considered an ideal choice for regenerating functional organs/tissues. Herein, we review recent developments in dECM scaffolds and put forward perspectives accordingly, with particular focus on the concept and fabrication of decellularized scaffolds, as well as the application of decellularized scaffolds and their combinations with stem cells (recellularized scaffolds) in tissue engineering, including skin, bone, nerve, heart, along with lung, liver and kidney.

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Section: Classification and Fabrication Of Decm Scaffoldsmentioning

confidence: 99%

Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering

Zhang

Chen

Hong

et al. 2022

Bioactive Materials

333

263

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“…Finding a suitable method to obtain a biocompatible scaffold with optimal biomechanical and structural properties is still a challenge in the field of tissue engineering. The literature data [ 51 , 52 ] available so far illustrate the great diversity among the favored methods of tissue removal and crosslinking. This is dictated by different experimental conditions, tissue types, and the purpose for which the scaffold will be used.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, the most popular and extensively investigated protocols regarding decellularization processes include chemical and enzymatic methods [ 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 ]. However, there are lately emerging reports [ 51 ] indicating that these conventional methods used for decellularization are harsh in nature and destructive to essential components of the ECM structure.…”

Section: Introductionmentioning

confidence: 99%

“…The known physical methods applied in decellularization processes are: thermal shock, mechanical pressure, electroporation, perfusion, pressure gradient, superficial fluids, ultrasonic waves, immersion and agitation, and vacuum-assisted [ 51 ]. Yet, none of the current tissue engineering strategies have considered laser or proton irradiation as effective methods for decellularized scaffolds production [ 52 ].…”

Section: Introductionmentioning

confidence: 99%

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Interdisciplinary Methods for Zoonotic Tissue Acellularization for Natural Heart Valve Substitute of Biomimetic Materials

et al. 2022

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The goal of this work was to create a bioactive tissue-based scaffold using multi-disciplinary engineering materials and tissue engineering techniques. Materials & methods: Physical techniques such as direct laser interference lithography and proton radiation were selected as alternative methods of enzymatic and chemical decellularization to remove cells from a tissue without degradation of the extracellular matrix nor its protein structure. This study was an attempt to prepare a functional scaffold for cell culture from tissue of animal origin using new physical methods that have not been considered before. The work was carried out under full control of the histological and molecular analysis. Results & conclusions: The most important finding was that the physical methods used to obtain the decellularized tissue scaffold differed in the efficiency of cell removal from the tissue in favour of the laser method. Both the laser method and the proton method exhibited a destructive effect on tissue structure and the genetic material in cell nuclei. This effect was visible on histology images as blurred areas within the cell nucleus. The finite element 3D simulation of decellularization process of the three-layer tissue of animal origin sample reflected well the mechanical response of tissue described by hyperelastic material models and provided results comparable to the experimental ones.

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“…Also, it can improve the removal of cellular remnant materials. The pressure leads to minimal changes in ECM structure, even less than agitation ( Crapo et al, 2011 ; Heath, 2019 ; Rabbani et al, 2021 ). Sonication is another physical method and can be considered a subtype of agitation.…”

Section: Decellularization Agents and Methodsmentioning

confidence: 99%

Decellularization in Tissue Engineering and Regenerative Medicine: Evaluation, Modification, and Application Methods

Neishabouri

Khaboushan

Daghigh

et al. 2022

Front. Bioeng. Biotechnol.

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Reproduction of different tissues using scaffolds and materials is a major element in regenerative medicine. The regeneration of whole organs with decellularized extracellular matrix (dECM) has remained a goal despite the use of these materials for different purposes. Recently, decellularization techniques have been widely used in producing scaffolds that are appropriate for regenerating damaged organs and may be able to overcome the shortage of donor organs. Decellularized ECM offers several advantages over synthetic compounds, including the preserved natural microenvironment features. Different decellularization methods have been developed, each of which is appropriate for removing cells from specific tissues under certain conditions. A variety of methods have been advanced for evaluating the decellularization process in terms of cell removal efficiency, tissue ultrastructure preservation, toxicity, biocompatibility, biodegradability, and mechanical resistance in order to enhance the efficacy of decellularization methods. Modification techniques improve the characteristics of decellularized scaffolds, making them available for the regeneration of damaged tissues. Moreover, modification of scaffolds makes them appropriate options for drug delivery, disease modeling, and improving stem cells growth and proliferation. However, considering different challenges in the way of decellularization methods and application of decellularized scaffolds, this field is constantly developing and progressively moving forward. This review has outlined recent decellularization and sterilization strategies, evaluation tests for efficient decellularization, materials processing, application, and challenges and future outlooks of decellularization in regenerative medicine and tissue engineering.

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Contribution of physical methods in decellularization of animal tissues

Cited by 44 publications

References 1 publication

Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering

Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering

Interdisciplinary Methods for Zoonotic Tissue Acellularization for Natural Heart Valve Substitute of Biomimetic Materials

Decellularization in Tissue Engineering and Regenerative Medicine: Evaluation, Modification, and Application Methods

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