Contribution and prognostic value of TSGA10 gene expression in patients with acute myeloid leukemia (AML)

Hoseinkhani, Zohreh; Rastegari-Pouyani, Mohsen; Oubari, Farhad; Mozafari, Hadi; Rahimzadeh, Amir Bahman; Maleki, Afshin; Amini, Saeideh; Mansouri, Kamran

doi:10.1016/j.prp.2019.01.003

Cited by 10 publications

(10 citation statements)

References 25 publications

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“…The negative relationship between expression of TSGA10 and HIF-α subunits was also verified in both cervical and breast cancer [32]. In acute myeloid leukemia, the interaction between TSGA10 and HIF1-α leads to a decrease in VEGF secretion [29]. This suggests there may be a feedback loop among HIF1-α, miR-10b-3p and TSGA10 in ESCC cells, which deserves further exploration.…”

Section: Discussionmentioning

confidence: 97%

“…TSGA10 is initially characterized as a testis-specific protein and tumor-associated antigen that facilitates angiogenesis and metastasis in various cancers [27]. The prognostic value of TSGA10 is also identified in acute myeloid leukemia and transitional cell carcinoma of the bladder [28, 29]. In ESCC, TSGA10 down-regulation is shown to be associated with a progressive clinical stage, and in vivo assays suggest TSGA10 knockdown significantly accelerates tumor growth and leads to larger tumor volumes [30].…”

Section: Discussionmentioning

confidence: 99%

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Hypoxia-induced microRNA-10b-3p promotes esophageal squamous cell carcinoma growth and metastasis by targeting TSGA10

Zhang

et al. 2019

Aging

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Evidence has shown that hypoxia promotes esophageal squamous cell carcinoma (ESCC) growth and metastasis, but the molecular mechanisms underlying that response remain poorly understood. MicroRNAs (miRNAs) are post-transcriptional regulators that participate in various cancer-related processes. Here, we demonstrated that hypoxia along with hypoxia-inducible factor 1α significantly increased expression of miR-10b-3p. Inhibition of miR-10b-3p weakened the effects of hypoxia on ESCC cell proliferation, migration and invasion, while miR-10b-3p overexpression had the opposite effects. Mechanistically, miR-10b-3p acted as cancer-promoting gene by targeting testis specific 10. Using a xenograft model, we observed that administration of miR-10b-3p agomir to tumors enhanced their growth and metastasis in vivo. These findings verified the potent regulatory role played by hypoxia-induced miR-10b-3p expression in ESCC progression. These results suggest that miR-10b-3p may be a useful therapeutic target for treating ESCC.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Hypoxia-induced microRNA-10b-3p promotes esophageal squamous cell carcinoma growth and metastasis by targeting TSGA10

Zhang

et al. 2019

Aging

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“…Reverse transcription for 500 ng of the total RNA extracted from all samples was performed using both random hexamer and oligo‐dT primers. The sense and antisense primers for genes of target and reference were designed using robust oligo primer design software (synthesized by the Bioneer Company) . The sequences of the oligonucleotide primers for qPCR analysis are listed in Table .…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA was extracted from the testis tissue samples both from patients and the control group using Trizol reagent (Invitrogen, Carldbad, CA, USA) in accordance with the manufacturer's instructions. 16 The quality and quantity of each sample were assessed using a NanoDropND-2000 Spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA), and their integrity was confirmed by electrophoresis on 1% agarose gel (Ultra-Pure TM Agarose; Invitrogen), and then they underwent Gel Red RNA staining, followed by detection under an ultraviolet light. The high-quality RNA samples with no degradation were stored at −80 C and the first-strand cDNA was synthesized using a cDNA synthesis kit (Fermentas, Waltham, MA, USA).…”

Section: Quantitative Real-time Polymerase Chain Reaction (Qpcr) Anmentioning

confidence: 99%

“…The sense and antisense primers for genes of target and reference were designed using robust oligo primer design software (synthesized by the Bioneer Company). 16 The sequences of the oligonucleotide primers for qPCR analysis are listed in Table 1. RPL13A and GAPDH were used as reference genes for normalization.…”

Section: Quantitative Real-time Polymerase Chain Reaction (Qpcr) Anmentioning

confidence: 99%

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Autophagy related gene expression status in patients diagnosed with azoospermia: A cross‐sectional study

Asgari

Bakhtiari

Rezazadeh

et al. 2020

The Journal of Gene Medicine

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Background Autophagy affects various aspects of the male reproductive system. Any defects in this process may lead to azoospermia. However, the exact molecular mechanisms of the autophagy pathway have remained largely obscure. Therefore, the present study aimed to investigate levels of autophagy pathway gene expression (i.e. Lc3B, Beclin1, ATG5 and Bcl2) in azoospermic patients. Methods The levels of Lc3B, Beclin1, ATG5 and Bcl2 mRNA expression in azoospermic patients and fertile males were evaluated by a real‐time polymerase chain reaction technique. In addition, diagnostic evaluation based on the receiver‐operating characteristic (ROC) curve was performed. Results The results obtained showed the decreased expression of Lc3B, Beclin1 and ATG5 genes in infertile patients compared to the control group (p < 0.05), whereas Bcl2 expression was increased in samples (p < 0.05). A diagnostic evaluation by ROC curve and calculation of the area under the curve showed that, using a cut‐off relative quantification of 4.550, 0.052, 0.056 and 0.012, the sensitivity of Lc3B, Beclin1, ATG5 and Bcl2 genes was 87.5%, 93.8%, 93.8% and 90%, respectively. In addition, a specificity of 76.7%, 76.7%, 93.3% and 81.2%, respectively, was observed. Conclusions As a first study, the current research suggests that an alteration in the expression of autophagy pathway genes may be associated with male infertility. Based on our finding, the increased expression of Bcl2 and formation of Becline1/Bcl2 complex, which inhibits Beclin1 recruitment, may lead to a decrease of the autophagy process in azoospermic patients. Accordingly, upon further investigation, the autophagy could be considered as an important aspect during spermatogenesis.

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Exosome: From leukemia progression to a novel therapeutic approach in leukemia treatment

et al. 2020

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Exosomes, as small vesicles, are released by tumor cells and tumor microenvironment (cells and function as key intercellular mediators and effects on different processes including tumorigenesis, angiogenesis, drug resistance, and evasion from immune system. These functions are due to exosomes' biomolecules which make them as efficient markers in early diagnosis of the disease. Also, exosomes have been recently applied in vaccination. The potential role of

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Contribution and prognostic value of TSGA10 gene expression in patients with acute myeloid leukemia (AML)

Cited by 10 publications

References 25 publications

Hypoxia-induced microRNA-10b-3p promotes esophageal squamous cell carcinoma growth and metastasis by targeting TSGA10

Hypoxia-induced microRNA-10b-3p promotes esophageal squamous cell carcinoma growth and metastasis by targeting TSGA10

Autophagy related gene expression status in patients diagnosed with azoospermia: A cross‐sectional study

Exosome: From leukemia progression to a novel therapeutic approach in leukemia treatment

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