Hypoxia-induced microRNA-10b-3p promotes esophageal squamous cell carcinoma growth and metastasis by targeting TSGA10

Zhang, Qiang; Zhang, Jingjing; Fu, Zhanzhao; Liu, Dong; Tang, Yong; Xu, Chengcheng; Wang, Haifeng; Zhang, Tao; Wu, Yue; Dong, Chao; Shao, Shasha; Wang, Guangxia

doi:10.18632/aging.102462

Cited by 29 publications

(24 citation statements)

References 30 publications

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“…Hypoxia is a typical characteristic observed in the tumor microenvironment and drives the aggressiveness of many tumors, such as hepatocellular carcinoma (Kung-Chun Chiu et al, 2019), colorectal cancer (Qureshi-Baig et al, 2019), and esophageal squamous cell carcinoma (Zhang et al, 2019). Under hypoxic conditions, many transcription factors are activated in tumor cells, inducing various downstream signals regulating cell proliferation, motility, and apoptosis (Semenza, 2012).…”

Section: Introductionmentioning

confidence: 99%

Identification of a Hypoxia-Associated Signature for Lung Adenocarcinoma

Cao

et al. 2020

Front. Genet.

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Background: A hypoxia microenvironment plays a role in the initiation and progression of many cancer types, but its involvement in lung adenocarcinoma is still unclear. This study aimed to explore the potential correlation between hypoxia and lung adenocarcinoma and establish the hypoxia-associated gene signature in lung adenocarcinoma. Methods: Lung adenocarcinoma cases were retrieved from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases. The genes to be included in the hypoxia-associated signature were selected by performing univariate Cox regression analysis and lasso regression analysis. Then, the gene signature was verified by performing a survival analysis and constructing the multiple receiver operating characteristic (ROC) curve. The CIBERSORT tool was then used to investigate the potential correlation between the gene signature and immune cells. Moreover, a nomogram was constructed and evaluated by calculating the C-index. Results: Four genes (XPNPEP1, ANGPTL4, SLC2A1, and PFKP) were included in the final signature. The results showed that patients in the high-risk group showed worse survival than those in the low-risk group. Moreover, we found two types of immune cells (memory activated CD4 + T cell and M0 macrophages) which showed a significant infiltration in the tissues of the high-risk group patients. Conclusion: The hypoxia-associated gene signature established and validated in this study could be used as a potential prognostic factor in lung adenocarcinoma and may guide the immunotherapy choice.

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Section: Introductionmentioning

confidence: 99%

Identification of a Hypoxia-Associated Signature for Lung Adenocarcinoma

Cao

et al. 2020

Front. Genet.

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“…It could be enriched in exosomes of hepatocellular carcinoma cells and was a potential marker for the diagnosis of early‐stage hepatocellular carcinoma 14 . Hypoxia could induce the expression of miR‐10b‐3p and promote the growth and metastasis of esophageal squamous cell carcinoma by targeting TSGA10 15 . It could also improve the invasion of PDAC through suppressed TIP30 and increased EGF 16 .…”

Section: Discussionmentioning

confidence: 99%

MiR‐10b inhibits migration and invasion of pancreatic ductal adenocarcinoma via regulating E2F7

Cui

2020

Clinical Laboratory Analysis

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Background Abnormal microRNAs (miRNAs) expression is closely related to the development and poor prognosis of pancreatic ductal adenocarcinoma (PDAC). We aimed to elucidate the invasive mechanism and clinical significance of miR‐10b in PDAC. Methods The RNA sequence data of pancreatic cancer were extracted from the TCGA database. R packages were performed to analyze the differential expression of RNAs. TargetScan, picTar, and miRanda were used to predict the target gene of miRNA. The expression level of the selected candidate was tested by western blot and RT‐PCR in PDAC cells and tissues. Scrape and Transwell assays were determined the effect of candidate molecules on cell migration and invasion. The gain of function and loss of function was achieved by co‐culture with mimics and vector. Luciferase reporters were generated based on the psiCHECK2 vector. The relative luciferase activity was measured with the Dual‐Luciferase Reporter Assay System and Infinate M200 PRO microplate reader. Results Based on the TCGA data and bioinformatics analysis, we obtained seven differentially expressed miRNAs. Both TCGA data and our center clinical date indicated that miR‐10b was contributed to the poor survival of PDAC. Based on the target gene prediction database, we found that E2F7 was a target mRNA of miR‐10b. In subsequent experiments in molecular biology, miR‐10b expression was downregulated in PDAC cells and tissues, while E2F7 was upregulated. Scrape and Transwell assay indicated that miR‐10b could inhibit the invasion and migration of PDAC. MiR‐10b was confirmed to be by the E2F7 targeting site by dual‐luciferase report. Moreover, rescue experiments prove that miR‐10b could inhibit the invasion and migration of PDAC cells by regulating E2F7 expression. Conclusion Our results suggest that miR‐10b could inhibit the progression of PDAC by regulating E2F7 expression and acts as an independent prognostic risk factor for PDAC.

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“…Recent studies have revealed that dysregulation of miR-NAs is common in cancer and that miRNAs can function as oncogenes or tumour suppressers [24][25][26][27][28]. Genomewide expression profiling of miRNAs using microarray analysis of tumour tissue and matched normal tissues has recognized hundreds of downregulated and upregulated miRNAs between two groups [29,30].…”

Section: Discussionmentioning

confidence: 99%

miR-363 suppresses the proliferation, migration and invasion of clear cell renal cell carcinoma by downregulating S1PR1

et al. 2020

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Background: MicroRNAs (miRNAs) serve as important regulators of the tumorigenesis and progression of many human cancers. Therefore, we evaluated the biological function and underlying mechanism of miR-363 in clear cell renal cell carcinoma (ccRCC). Methods: The expression of miR-363 in ccRCC tissues compared with adjacent normal renal tissues was detected by quantitative real-time polymerase chain reaction, and the association between miR-363 levels and prognosis of ccRCC patients was analyzed. The candidate target gene of miR-363 was determined by in silico analysis and luciferase reporter assays. The effects of miR-363 on the proliferation, migration and invasion of ccRCC cells in vitro were determined by MTS assay, colony formation assay, Transwell assay and wound healing assay. We also investigated the roles of miR-363 in vivo by a xenograft tumour model. The mechanism of miR-363 on the proliferation, migration and invasion of ccRCC was determined by gain-and loss-of-function analyses. Results: we demonstrated that miR-363 expression was obviously downregulated in ccRCC tissues and that reduced miR-363 expression was correlated with poor disease-free survival (DFS) in ccRCC patients after surgery. S1PR1 expression was inversely correlated with the level of miR-363 in human ccRCC samples. Luciferase reporter assays suggested that S1PR1 was a direct functional target of miR-363. miR-363 downregulated S1PR1 expression and suppressed the proliferation, migration and invasion abilities of ccRCC cells in vitro and suppressed xenograft tumour growth in vivo. Importantly, miR-363 exerted its biological function by inhibiting S1PR1 expression in ccRCC cells, leading to the repression of ERK activation. Moreover, we found that the levels of downstream effectors of ERK, including PDGF-A, PDGF-B, and epithelial-mesenchymal transition (EMT)-related genes, were decreased after miR-363 overexpression. Conclusions: Our results suggest that miR-363 acts as a tumour suppressor by directly targeting S1PR1 in ccRCC and may be a potential new therapeutic target for ccRCC.

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Hypoxia-induced microRNA-10b-3p promotes esophageal squamous cell carcinoma growth and metastasis by targeting TSGA10

Cited by 29 publications

References 30 publications

Identification of a Hypoxia-Associated Signature for Lung Adenocarcinoma

Identification of a Hypoxia-Associated Signature for Lung Adenocarcinoma

MiR‐10b inhibits migration and invasion of pancreatic ductal adenocarcinoma via regulating E2F7

miR-363 suppresses the proliferation, migration and invasion of clear cell renal cell carcinoma by downregulating S1PR1

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