Contrasting Results from Two Commercial Kits Testing for the Presence of Clostridium perfringens Enterotoxin in Feces from Norovirus-Infected Human Patients

Ishioka, Taisei; Aihara, Yoshiyuki; Carle, Yuki; Shigemura, Hiroaki; Kubomura, Akiko; Motoya, Takumi; Nakamoto, Arimi; Nakamura, Asako; Fujimoto, Shuji; Hirai, Shinichiro; Oishi, Kazunori; Nagaoka, Hiromi; Kimura, Hirokazu; Murakami, Koichi

doi:10.7754/clin.lab.2019.190801

Cited by 3 publications

(2 citation statements)

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“…This finding is consistent with the finding from 1998 to 2008, meat, especially beef was incriminated as the most common vehicle of transmission of this bacterium as revealed by food poisoning outbreaks due to C. perfringens in the United States [9]. It has been found that an ELISA-based testing approach may be more reliable than RPLA assays for CPE detection [10]. Hence, the usage of PET-RPLA with a sensitivity of 2ng/ml and any level of toxin production has given a negative result been a limitation of the present study.…”

Section: Discussionsupporting

confidence: 90%

Selecting out of virulent strains of C. perfringens by the preparation process of meat curries possess a public health threat: evidence from a retrospective study

Perera

Ranasinghe

2023

Preprint

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Heat resistant spore formation is favourable for selecting out of C. perfringens virulent strains by the preparation process of meat curries. When heat resistance coupled with enterotoxin production it possesses a public health threat. Hence, this study was conducted using a representative sub sample of stored 15 meat curry isolates of C.perfringens from a large sample based on a previous descriptive cross sectional study to compare heat-resistant spore (HRS) formation, C. perfringens enterotoxin (CPE) production, and combined HRS - CPE abilities of meat curry isolates of this bacterium obtained from eating houses within Colombo city. Representative subsample (12 chicken and 03 beef) of preserved C. perfringens cultures after complete confirming retrieved from the cold room. Stored culture was vortex mixed prior to inoculation. Subsequently, 20ml of freshly steamed thioglycollate medium was inoculated with 2ml of stored culture (preserved in cooked meat medium and they were incubated anaerobically at 370C for 24hrs. Sporulation endeavoured using 3 media: Duncan and Strong, Modified Duncan and Strong, and Sporulation Broth as described previously. Heat resistance was tested at 1000C for 1 h in distilled water and the production of enterotoxin was evaluated with a PET-RPLA kit as detailed previously. The differences in selected virulent factors by the variety of meat curry were statistically significant (p<0.05). Selecting out of virulent strains due to the process of curry preparation was evident from this retrospective study. This evidence needs to confirm with a large sample size refining methodologies.

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Section: Discussionsupporting

confidence: 90%

Selecting out of virulent strains of C. perfringens by the preparation process of meat curries possess a public health threat: evidence from a retrospective study

Perera

Ranasinghe

2023

Preprint

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show abstract

“…17,36 Indeed, several studies reported that C. perfringens infection was diagnosed by detection of CPE toxin using ELISA, reversed passive latex agglutination (RPLA) and enzyme immunoassay (EIA) tests. 28,37,38…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Genetic Diversity of Clostridium perfringens Isolates in Hospitalized Diarrheal Patients from Central China

Wang¹,

Dong²,

Ma³

et al. 2021

IDR

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Proteomic identification and quantification of Clostridium perfringens enterotoxin using a stable isotope-labelled peptide via liquid chromatography–tandem mass spectrometry

et al. 2023

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Contrasting Results from Two Commercial Kits Testing for the Presence of Clostridium perfringens Enterotoxin in Feces from Norovirus-Infected Human Patients

Cited by 3 publications

References 0 publications

Selecting out of virulent strains of C. perfringens by the preparation process of meat curries possess a public health threat: evidence from a retrospective study

Selecting out of virulent strains of C. perfringens by the preparation process of meat curries possess a public health threat: evidence from a retrospective study

Prevalence and Genetic Diversity of Clostridium perfringens Isolates in Hospitalized Diarrheal Patients from Central China

Proteomic identification and quantification of Clostridium perfringens enterotoxin using a stable isotope-labelled peptide via liquid chromatography–tandem mass spectrometry

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