Prevalence and Genetic Diversity of Clostridium perfringens Isolates in Hospitalized Diarrheal Patients from Central China

Wang, Baoya; Dong, Wenjuan; Ma, Liyan; Dong, Yong-hui; Wang, Shanmei; Yuan, Youhua; Ma, Qiang; Xu, Jin; Yan, Wenjuan; Jiang, Nan; Zhang, Qi; Xu, Wenbo; Ma, Bing; Chu, Yafei; Zhang, Jiangfeng; Li, Li; Li, Yang

doi:10.2147/idr.s338593

Cited by 5 publications

(11 citation statements)

References 38 publications

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“…In our study, the predominant STs from human isolates (ST221, ST62, ST408) were significantly distinct from the predominant STs (ST479) from animal isolates, a result that was consistent with previous research on global C. perfringens strains, indicating a diverse distribution of C. perfringens among multiple sources (18,19). In global surveys, a clonal complex (CC) represented by three STs (ST 98, ST 41, and ST 110) predominantly representing type F (18/20 strains) was mostly associated with human illnesses (18). Among the predominant STs, ST 54 was associated with enteritis cases in foals and dogs, and ST 58 was associated with necrotic enteritis in poultry (18).…”

Section: Discussionsupporting

confidence: 92%

“…The copyright holder for this this version posted September 24, 2022. ; https://doi.org/10.1101/2022.09.23.509295 doi: bioRxiv preprint ST77 were associated with human illnesses (18,19). Consistent with these findings, we observed that the strains ST41 (2/2, type A) and ST77 (2/2, type F) were isolated from patients with diarrhea.…”

Section: Discussionsupporting

confidence: 86%

“…MLST analysis revealed that 186 C. perfringens isolates exhibited 135 sequence types (STs) with a high degree of genetic diversity, including 93 new STs. In the segments of colA (18), groEL (16), sodA (20), plc (23), gyrB (13), sigK (10), pgk (9), and nadA (20), up to 129 new alleles were identified, resulting in new genotypes. Among the 132 STs, the most frequent ST was ST221 (7/186, 3.74%), followed by ST408 (5/186, 2.67%), ST479 (5/186, 2.67%), ST62 (4/186, 2.14%), and ST493 (4/186, 2.14%).…”

Section: Resultsmentioning

confidence: 99%

“…In global surveys, a clonal complex (CC) represented by three STs (ST 98, ST 41, and ST 110) predominantly representing type F (18/20 strains) was mostly associated with human illnesses (18). Among the predominant STs, ST 54 was associated with enteritis cases in foals and dogs, and ST 58 was associated with necrotic enteritis in poultry (18). In our study, most type F strains (10/11 strains) were associated with human illnesses, different from the results of previous studies.…”

Section: Discussionmentioning

confidence: 99%

Section: Evolution and Phylogenetic Analysis Of C Perfringens Isolate...mentioning

confidence: 99%

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Molecular characters and phylogenetic analysis of Clostridium perfringens from different regions in China, from 2013 to 2021

Zhong

Zheng

Wang

et al. 2022

Preprint

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Clostridium perfringens (C. perfringens) is a significant foodborne pathogen and a common cause of intestinal diseases in both animals and humans. Altogether, 186 isolates were obtained from humans (n = 147), animals (n = 25), and food (n = 14), comprising 174 type A strains (93.55%), 11 type F strains (5.91%), and one type D strain (0.54%); and were analyzed by multilocus sequence typing (MLST) and antimicrobial susceptibility testing. Additionally, some specific ST complexes were analyzed by cgMLST and cgSNP to investigate genetic relatedness. MLST indicated the most prevalent STs of C. perfringens of human and animal origin were as follows: ST221 (5/147), ST62 (4/147), ST408 (4/147), and ST493 (4/147) were predominant in humans, while ST479 (5/25) was the major type in animals. Within the same ST complex, genetically unrelated relationships or potential clustering/transmission events were further recognized by cgMLST and cgSNP, illustrating that these two methods are valuable in defining outbreaks and transmission events. All tested isolates were susceptible to vancomycin and meropenem. The rates of resistance to metronidazole, penicillin, cefoxitin, moxifloxacin, and clindamycin were low (metronidazole: 1.08%; penicillin: 9.68%; cefoxitin: 0.54%; moxifloxacin: 6.45%; and chloramphenicol: 3.76%). Interestingly, 49.66% of human origin were clindamycin-resistant, and 18.2% were penicillin-insensitive. Importantly, the portion of MDR isolates was significantly lower than in previous reports. The study provides an overview of the epidemiological characteristics of C. perfringens with different origins and hosts in China. C. perfringens demonstrated remarkable genetic diversity and distinct molecular features compared to antibiotic-resistance profiles from other studies.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Evolution and Phylogenetic Analysis Of C Perfringens Isolate...mentioning

confidence: 99%

See 3 more Smart Citations

Molecular characters and phylogenetic analysis of Clostridium perfringens from different regions in China, from 2013 to 2021

Zhong

Zheng

Wang

et al. 2022

Preprint

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Clostridium perfringens in central Colombia: frequency, toxin genes, and risk factors

Camargo,

Bohorquez,

López

et al. 2024

Gut Pathog

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Clostridium perfringens is an opportunistic bacterium that causes intestinal diseases in both humans and animals. This study aimed to assess the frequency of C. perfringens and the presence of toxin-encoding genes in fecal samples from individuals with or without gastrointestinal symptoms in the Department of Boyacá, Colombia. Additionally, risk factors associated with carriage and disease development were analyzed. A total of 114 stool samples were analyzed using a molecular test based on specific polymerase chain reaction (PCR) targeting 16S-rRNA and alpha toxin (cpa) genes. For individuals with a positive result for the PCR test, stool samples were cultured on Tryptose Sulfite Cycloserine (TSC) agar. Two to five colonies forming units were selected based on phenotypic characteristics, resulting in 56 bacterial isolates. These isolates were then analyzed for toxin-coding genes associated with gastrointestinal diseases. In addition, sociodemographic and clinical data from 77 individuals were also analyzed. The overall frequency of C. perfringens was 19.3% (n = 22/114). The detection frequency in 77 individuals with clinical data was 16.6% (n = 5/30) among symptomatic individuals and 21.2% (n = 10/47) among asymptomatic individuals. All 56 isolates obtained carried the cpa gene, while cpb2 was present in 10.7% (n = 6/56); cpe and cpb genes were not detected. Notably, diabetes and autoimmune diseases are significantly associated with an increased risk of C. perfringens detection (adjusted OR 8.41: 95% CI 1.32–35.89). This study highlights an elevated frequency of C. perfringens and the presence of the cpb2 gene in asymptomatic individuals compared with their symptomatic counterparts. These findings offer insights into the distribution and virulence factors of C. perfringens at a micro-geographical level. This information supports the need for developing tailored prevention strategies based on local characteristics to promote active surveillance programs based on molecular epidemiology.

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Molecular characteristics and phylogenetic analysis of Clostridium perfringens from different regions in China, from 2013 to 2021

et al. 2023

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Clostridium perfringens (C. perfringens) is a significant foodborne pathogen and a common cause of intestinal diseases in both animals and humans. Our study investigated MLST, phenotypic antimicrobial resistance profiles, and resistance genes among isolates from human, animal and food. 186 C. perfringens isolates were obtained from nine provinces in China between 2013 and 2021. Additionally, some specific ST complexes were analyzed by cgMLST and cgSNP to investigate genetic relatedness. MLST indicated the most prevalent STs of C. perfringens of human and animal origin were as follows: ST221 (5/147), ST62 (4/147), ST408 (4/147), and ST493 (4/147) were predominant in humans, while ST479 (5/25) was the major type in animals. Within the same ST complex, genetically unrelated relationships or potential clustering/transmission events were further recognized by cgMLST and cgSNP, illustrating that these two methods are valuable in defining outbreaks and transmission events. All tested isolates were susceptible to vancomycin and meropenem. The rates of resistance to metronidazole, penicillin, cefoxitin, moxifloxacin, and chloramphenicol were low (metronidazole: 1.08%; penicillin: 9.68%; cefoxitin: 0.54%; moxifloxacin: 6.45%; and chloramphenicol: 3.76%). Interestingly, 49.66% of human origin were clindamycin-resistant, and 18.2% were penicillin-insensitive. Importantly, the portion of MDR isolates was significantly lower than in previous reports. The study provides an overview of the epidemiological characteristics of C. perfringens with different origins and hosts in China. C. perfringens demonstrated remarkable genetic diversity and distinct molecular features compared to antibiotic-resistance profiles from other studies.

show abstract

Prevalence and Genetic Diversity of Clostridium perfringens Isolates in Hospitalized Diarrheal Patients from Central China

Cited by 5 publications

References 38 publications

Molecular characters and phylogenetic analysis of Clostridium perfringens from different regions in China, from 2013 to 2021

Molecular characters and phylogenetic analysis of Clostridium perfringens from different regions in China, from 2013 to 2021

Clostridium perfringens in central Colombia: frequency, toxin genes, and risk factors

Molecular characteristics and phylogenetic analysis of Clostridium perfringens from different regions in China, from 2013 to 2021

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