Contrast transfer functions for Zernike phase contrast in full-field transmission hard X-ray microscopy

Yang, Yang; Cheng, Yin; Heine, Ruth; Baumbach, Tilo

doi:10.1364/oe.24.006063

Cited by 5 publications

(4 citation statements)

References 30 publications

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“…This set includes a number of spectroscopic techniques such as XRF, XAS, and X-ray emission spectroscopy (XES), among others. There are also scattering techniques, such as powder X-ray diffraction (PXRD), total X-ray scattering, or SAXS imaging, X-ray phase contrast imaging techniques, − and X-ray photon correlation spectroscopy (XPCS). − These techniques could be used to probe nanomaterials directly, including NP-based drugs, and to provide important information about their location, quantification, state, and supramolecular arrangements.…”

Section: X-ray-based Techniques As An Alternative To Study Nanopartic...mentioning

confidence: 99%

“…In contrast to soft X-rays, the greater biological penetration depth of hard X-rays enables imaging of larger cells, tissues, and organisms. Furthermore, the morphology of tissues and cells can be visualized with hard X-rays through use of chemical staining to enhance the signal of organic structures of cells and tissue, but also by using phase contrast data acquisition protocols, − including holotomography, or coherent diffraction-based ptychography . The morphology and organelle localization can also be assessed on cellular samples by using experimental approaches based on the correlative acquisition of optical and hard X-ray microscopy images .…”

Section: X-ray Imaging Of Nanoparticle-based Drugs (And Related Syste...mentioning

confidence: 99%

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X-ray-Based Techniques to Study the Nano–Bio Interface

et al. 2021

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X-ray-based analytics are routinely applied in many fields, including physics, chemistry, materials science, and engineering. The full potential of such techniques in the life sciences and medicine, however, has not yet been fully exploited. We highlight current and upcoming advances in this direction. We describe different X-ray-based methodologies (including those performed at synchrotron light sources and X‑ray free-electron lasers) and their potentials for application to investigate the nano–bio interface. The discussion is predominantly guided by asking how such methods could better help to understand and to improve nanoparticle-based drug delivery, though the concepts also apply to nano–bio interactions in general. We discuss current limitations and how they might be overcome, particularly for future use in vivo.

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Section: X-ray-based Techniques As An Alternative To Study Nanopartic...mentioning

confidence: 99%

Section: X-ray Imaging Of Nanoparticle-based Drugs (And Related Syste...mentioning

confidence: 99%

X-ray-Based Techniques to Study the Nano–Bio Interface

et al. 2021

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“…Sensitivity to element speci city and oxidation states is achieved by collecting a series of 2D images across an absorption edge [5][6][7]. Zernike contrast can be employed to enhance the detection sensitivity of low-Z elements [8,9]. Both TXMs and nanoprobes serve an important role in nanoscale imaging.…”

Section: Imagingmentioning

confidence: 99%

Future trends in synchrotron science at NSLS-II

Hill

Campbell²,

Carini

et al. 2020

J. Phys.: Condens. Matter

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In this paper, we summarize brie y some of the future trends in synchrotron science as seen at the National Synchrotron Light Source II, a new, low emittance source recently commissioned at Brookhaven National Laboratory. We touch upon imaging techniques, the study of dynamics, the increasing use of multimodal approaches, the vital importance of data science, and other enabling technologies. Each are presently undergoing a time of rapid change, driving the eld of synchrotron science forward at an ever increasing pace. It is truly an exciting time and one in which Roger Cowley, to whom this journal issue is dedicated, would surely be both invigorated by, and at the heart of.

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“…Imaging cells or tissues thicker than this requires deformation of specimens, making them extended in one direction (for example flat or cylindrical objects that are thinner in some orientations) or trimming them to the suitable thickness as done in electron microscopy. Microscopy with hard x-rays overcomes the limitation on specimen thickness but relies on chemical staining or the use of phase contrast data acquisition protocols [84–86].…”

Section: Soft X-ray Microscopy Of Cellsmentioning

confidence: 99%

Imaging cell morphology and physiology using X-rays

Weinhardt

Chen

Ekman

et al. 2019

Biochemical Society Transactions

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Morphometric measurements, such as quantifying cell shape, characterizing sub-cellular organization, and probing cell-cell interactions, are fundamental in cell biology and clinical medicine. Until quite recently, the main source of morphometric data on cells has been light- and electron-based microscope images. However, a number of technological advances have propelled X-ray microscopy into becoming another source of high-quality morphometric information. Here we review the status of X-ray microscopy as a quantitative biological imaging modality. We also describe the combination of X-ray microscopy data with information from other modalities to generate polychromatic views of biological systems. For example, the amalgamation of molecular localization data, from fluorescence microscopy or spectromicroscopy, with structural information from X-ray tomography. This combination of data from the same specimen generates a more complete picture of the system than can be obtained by a single microscopy method. Such multimodal combinations greatly enhance our understanding of biology by combining physiological and morphological data to create models that more accurately reflect the complexities of life.

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Contrast transfer functions for Zernike phase contrast in full-field transmission hard X-ray microscopy

Cited by 5 publications

References 30 publications

X-ray-Based Techniques to Study the Nano–Bio Interface

X-ray-Based Techniques to Study the Nano–Bio Interface

Future trends in synchrotron science at NSLS-II

Imaging cell morphology and physiology using X-rays

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