Continuum of overlapping clones spanning the entire human chromosome 21q

Chumakov, Ilya; Rigault, Philippe; Guillou, Sophie; Ougen, Pierre; Billaut, Alain; Guasconi, G; Gervy, Patricia; LeGall, Isabelle; Soularue, Pascal; Grinas, Laurent; Bougueleret, Lydie; Bellanné‐Chantelot, Christine; Lacroix, Bruno; Barillot, Emmanuel; Gesnouin, Philippe; Pook, Stuart; Vaysseix, Guy; Frelat, G.; Schmitz, Annette; Sambucy, J.-L.; Bosch, Assumpcio; Estivill, Xavier; Weissenbach, Jean; Vignal, Alain; Riethman, Harold; Cox, David J.; Patterson, David; Gardiner, K.; Hattori, Masahira; Sakaki, Yoshiyuki; Ichikawa, Hitoshi; Ohki, Masafumi; Paslier, Denis Le; Heilig, Roland; Antonarakis, Stylianos E.; Cohen, Daniel

doi:10.1038/359380a0

Cited by 368 publications

(176 citation statements)

References 27 publications

(19 reference statements)

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“…To determine the nature of the observed altered fragments, a physical map ( Figure 2a) of the region containing these genes was constructed using DNA fragments from (HCX); fragment data from Table 1 were integrated with cosmid and yeast arti®cial chromosome (YAC) contig map data (Chumakov et al, 1992;Ochman and Buckholtz, 1995;Patil et al, 1994) to yield the map. 3' and 5'-ets-2 probes hybridized to adjacent 640 kb and 51000 kb NruI fragments, respectively, establishing a continuous region (within the resolution of fragment measurements) of 41.5 Mbp (Table 1, see HCX, Figure 1).…”

Section: Resultsmentioning

confidence: 99%

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Altered expression of Erg and Ets-2 transcription factors is associated with genetic changes at 21q22.2-22.3 in immortal and cervical carcinoma cell lines

1997

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Human Papillomavirus (HPV) type 16 is the most frequently detected HPV in cervical cancer. Although epidemiologic and experimental evidence indicates a prominent role for HPV infection in the development of this disease, other factors are also involved. Altered expression of the ets family transcription factors erg and ets-2 was found associated with the development of cervical carcinoma. Overexpression also occurred in a HPV-16-immortalized cervical cell line, CX16-2, which has HPV integrated at a translocation breakpoint t(19;21) involving 21q22.2-22.3, where these genes have been mapped. Six of 10 cervical carcinoma cell lines overexpressed ets-2 RNA suggesting an association of overexpression with cervical cell neoplasia. A clonally related pair of cervical carcinoma cell lines, C-4I and C-4II, showed di erential expression of erg and ets-2. C-4I overexpressed ets-2 RNA compared to normal cervical cells and C-4II. C-4II expressed a 5.3 kb erg transcript not seen in C-4I, ectocervical cells or other cervical carcinoma cell lines examined. Pulsed ®eld gel electrophoresis was used to analyse changes in DNA fragments related to structural changes and to construct a physical map encompassing erg and ets-2. Alterations in erg and ets-2 RNA expression in each of three di erent cell lines examined were associated with translocations. Association between altered expression of erg and ets-2 and altered regional structural suggests that these genes are important targets in cervical carcinogenesis.

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Section: Resultsmentioning

confidence: 99%

“…D21S55 (Tanzi et al, 1992) and GA5F11 (Chumakov et al, 1992) are sequence-tagged sites (STSs) de®ning unique loci, mapping to chromosome 21. D21S55 (208 bp) and GA5F11 (320 bp) PCR products were ampli®ed from placental DNA and puri®ed from gels.…”

Section: Probes and Hybridizationsmentioning

confidence: 99%

Altered expression of Erg and Ets-2 transcription factors is associated with genetic changes at 21q22.2-22.3 in immortal and cervical carcinoma cell lines

1997

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“…LIFR YAC clones were isolated from the CEPH mark 3 YAC library (Chumakov et al, 1992) by a combination of PCR-based screening and colony hybridization analysis (Green and Olson, 1990) using the primer set LIFR-NUP and LIFR-low (5'-TGG AGG GCG ATG AAT GAG TCG C-3'). YAC DNA was isolated and characterized as described before (Schoenmakers et al, 1994(Schoenmakers et al, , 1995a.…”

Section: Isolation Of Yac Clones and Fish Analysesmentioning

confidence: 99%

The recurrent translocation t(5;8)(p13;q12) in pleomorphic adenomas results in upregulation of PLAG1 gene expression under control of the LIFR promoter

et al. 1998

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We have previously shown that the PLAG1 gene on chromosome 8q12 is consistently rearranged in pleomorphic adenomas of the salivary glands with t(3;8)(p21;q12) translocations. The t(3;8) results in promoter swapping between the PLAG1 gene, which encodes a novel zinc ®nger protein, and the constitutively expressed gene for b-catenin (CTNNB1), a protein with roles in cell-cell adhesion and the WG/WNT signalling pathway. In order to assess the importance of other translocation partner genes of PLAG1, and their possible relationship to CTNNB1, we have characterized a second recurrent translocation, i.e. the t(5;8)(p13;q12). This translocation leads to ectopic expression of a chimeric transcript consisting of sequences from the ubiquitously expressed gene for the leukemia inhibitory factor receptor (LIFR) and PLAG1. As for the t(3;8), the fusions occurred in the 5'-noncoding regions of both genes, exchanging regulatory control elements while preserving the coding sequences. The results of the current as well as previous studies indicate that ectopic expression of PLAG1 under the control of promoters of distinct translocation partner genes is a general pathogenetic mechanism for pleomorphic adenomas with 8q12 aberrations.

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“…For example, physical mapping may consist of restriction fingerprinting (Coulson et al, 1986;Olson et al, 1986;Kohara et al, 1987;Carrano et al, 1989;Stallings et al, 1990;Trask et al, 1992), PCR-based STS content mapping (Green and Olson, 1990;Foote et al, 1992;Chumakov et al, 1992), and clone-to-clone hybridization and often incorporates maps and mapping reagents from external sources.…”

Section: Introductionmentioning

confidence: 99%

Integrated Mapping Package—A Physical Mapping Software Tool Kit

Zhang

Liao

et al. 1999

Genomics

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We have developed an integrated physical mapping computer software package (IMP), originally designed to support the physical mapping of human chromosome 13 and expanded to support several gene-identification projects based on the positional candidate approach. IMP displays map data in a form that provides useful guidelines to the end users. An integrated map with high resolution and confidence is constructed from different types of mapping data, including hybridization experiments, STS-based PCR assays, genetic linkage mapping, cDNA localization, and FISH data. The map is also designed to provide suggestions for specific experiments that are required to obtain maps with even higher resolution and confidence. To this end, the optimization employs multiple constraints that take into account already established STS "scaffold" maps. This software thus serves as an important general tool kit for physical mapping, sequencing, and gene-hunting projects.

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Continuum of overlapping clones spanning the entire human chromosome 21q

Cited by 368 publications

References 27 publications

Altered expression of Erg and Ets-2 transcription factors is associated with genetic changes at 21q22.2-22.3 in immortal and cervical carcinoma cell lines

Altered expression of Erg and Ets-2 transcription factors is associated with genetic changes at 21q22.2-22.3 in immortal and cervical carcinoma cell lines

The recurrent translocation t(5;8)(p13;q12) in pleomorphic adenomas results in upregulation of PLAG1 gene expression under control of the LIFR promoter

Integrated Mapping Package—A Physical Mapping Software Tool Kit

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