Continuous Synthesis of Glyceryl Ferulate Using Immobilized Candida antarctica Lipase

Abstract

Glyceryl ferulate was synthesized through condensation of ferulic acid and glycerol at 50 in glycerols with different water contents using an immobilized lipase from Candida antarctica in a batch reactor, and condensation in the glycerol with a 7.5% (w/w) water content was shown to be the favorite. The solubility of ferulic acid was higher at higher temperature in glycerol with a lower water content. The viscosity was lower at higher temperature for the glycerol with a higher water content. The condensation was carried out using a batch reactor at a temperature from 50 to 90 . These observations indicated that the condensation at 80 in the glycerol with a 7.5% (w/w) water content was the most adequate for continuously synthesizing glyceryl ferulate. A reactor system was constructed for the continuous synthesis and was steadily operated to realize a productivity of 430 kg/(m 3 -reactor(day) without any decrease in the conversion for at least 6 days.

Enzymatic synthesis of bioactive compounds with high potential for cosmeceutical application

“…A two-step synthesis of feruloylated diacylglycerols using CALB has been proposed by Sun et al ( 2007 ) including the transesterification of ethyl ferulate with glycerol and the subsequent transesterification of glyceryl ferulate with oleic acid offering high yield of products (up to 96 %). Esterification of FA to glyceryl ferulate by CALB has been performed in a continuous reactor reaching 80 % conversion and productivity of 430 kg/m 3 /reactor day (Matsuo et al 2008 ). Biocatalysis under vacuum-rotary evaporation contributes to increased conversion by eliminating external mass transfer resistance, effective interaction among different phases of enzymatic reaction, minimizing the negative effects of by-product ethanol (when ethyl ferulate is used as donor) on lipase activity (Xin et al 2009 ).…”

Hydrophilic Glyceryl Ferulates Preparation Catalyzed by Free Lipase B from <i>Candida antar</i><i>tica</i>

“…drophilic FG and glyceryl diferulate DFG preparation 36,37 . Matsuo et al also used immobilized Candida antarctica lipase B as a biocatalyst to prepare FG 16 . However, in these previous methods of FG preparation, the high cost of the immobilized Novozym 435 2,273$/kg and the adsorption of glycerol on the immobilized support limited their industrial applications.…”

Lipase-catalyzed syntheses of sugar esters in non-aqueous media

“…However, it is possible to perform a reaction without any adjuvant under limited conditions (solvent-free system). For example, glycerol, can be easily converted to acylglycerols by mixing with a fatty acid or an aromatic carboxylic acid in the presence of a lipase (Matsuo et al 2008;Pinsirodom et al 2004;Watanabe et al 2002Watanabe et al , 2005. In addition, diesters of glycerol can also be synthesized by mixing the substrates and lipase .…”