Continuous separation of cells by balanced dielectrophoretic forces at multiple frequencies

Braschler, Thomas; Demierre, Nicolas; Nascimento, Elisabete; Silva, Tiago; Oliva, Abel; Renaud, Philippe

doi:10.1039/b710303d

Cited by 122 publications

(87 citation statements)

References 28 publications

(38 reference statements)

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“…In addition to validating cell synchronization, these experiments also show yeast viability, since in general all the cells selected by the sorting procedure (N z 30 for convenient observation in an outlet channel) divide. For the medium conductivity used in the experiments described above, dead cells lack a cross-over in the frequency range considered, 20 thus selecting the cells with the most negative opacity value also eliminates the dead cells.…”

Section: Synchronizationmentioning

confidence: 99%

“…This opposition of dielectrophoretic forces eliminates the first order dependence of the force on the volume, 19 increasing the sensitivity to changes in cell shape throughout the division cycle. The arrangement has two additional important advantages: 18,20 First, the cell sorting takes place perpendicular to the flow, such that the device can be operated continuously; and second, the cells are pushed towards a characteristic equilibrium position for the different division stages, independently of the flow speed ( Fig. 1-B).…”

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confidence: 99%

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Tracking and synchronization of the yeast cell cycle using dielectrophoretic opacity

et al. 2011

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Cell cycle synchronization is an important tool for the study of the cell division stages and signalling. It provides homogeneous cell cultures that are of importance to develop and improve processes such as protein synthesis and drug screening. The main approach today is the use of metabolic agents that block the cell cycle at a particular phase and accumulate cells at this phase, disturbing the cell physiology. We provide here a non-invasive and label-free continuous cell sorting technique to analyze and synchronize yeast cell division. By balancing opposing dielectrophoretic forces at multiple frequencies, we maximize sensitivity to the characteristic shape and internal structure changes occurring during the yeast cell cycle, allowing us to synchronize the culture in late anaphase.

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Section: Synchronizationmentioning

confidence: 99%

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confidence: 99%

Tracking and synchronization of the yeast cell cycle using dielectrophoretic opacity

et al. 2011

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“…9 Additionally, transformed and nontransformed rat kidney cells, 10 malignant human breast cancer epithelial cells and benign breast epithelial cells, 11,12 and healthy and infected erythrocytes have all been shown to have different electrical properties. 13 Dielectrophoresis (DEP), the motion of a particle due to its polarization in the presence of a non-uniform electric field, 14 has been used to manipulate particles, including mixing, 15 separation, [16][17][18] enrichment, 19,20 detection, 21 and to investigate their specific electrical properties.…”

Section: Introductionmentioning

confidence: 99%

Investigating dielectric properties of different stages of syngeneic murine ovarian cancer cells

et al. 2013

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In this study, the electrical properties of four different stages of mouse ovarian surface epithelial (MOSE) cells were investigated using contactless dielectrophoresis (cDEP). This study expands the work from our previous report describing for the first time the crossover frequency and cell specific membrane capacitance of different stages of cancer cells that are derived from the same cell line. The specific membrane capacitance increased as the stage of malignancy advanced from 15.39 6 1.54 mF m À2 for a non-malignant benign stage to 26.42 6 1.22 mF m À2 for the most aggressive stage. These differences could be the result of morphological variations due to changes in the cytoskeleton structure, specifically the decrease of the level of actin filaments in the cytoskeleton structure of the transformed MOSE cells. Studying the electrical properties of MOSE cells provides important information as a first step to develop cancer-treatment techniques which could partially reverse the cytoskeleton disorganization of malignant cells to a morphology more similar to that of benign cells. V C 2013 American Institute of Physics.

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“…Along the horizontal plane, the shape and steepness of the gradient are controlled by adjusting the size and positions of the electrodes and of any insulating features in the channel that might further distort the field lines [10]. Alternatively, vertical electrodes of the same geometry on either side of a channel can be fabricated with dielectrophoretic steering forces provided by actuation of electrodes on either side of the channel with different frequencies or voltages [11], [12].…”

Section: A 3-d Electrodesmentioning

confidence: 99%

3-D In-Bi-Sn Electrodes for Lab-on-PCB Cell Sorting

Luo

Simon

Jiang

et al. 2016

IEEE Trans. Compon., Packag. Manufact. Technol.

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Abstract-We present a microfluidic lab-on-printed circuit board (PCB) device containing alloy vertical electrodes for sorting microparticles using dielectrophoresis. The device consists of a hydrodynamic prefocuser and an electronic sorting region. Lining the two sidewalls of the electronic sorting region are regularly spaced rectangular metal electrodes reaching from the floor to the ceiling of the flow channel that bridge electric field lines laterally across the channel. The size and distribution of these vertical electrodes are arranged asymmetrically such that the resultant electric field forms sharp electric field gradients across the channel; specific geometries were optimized using finite element methods. Particles entering the device are initially focused on a single stream as they pass through the prefocuser. Subsequently, they are exposed to the lateral electric field gradient and separate into streams based on their size and dielectric properties. Validation was performed by dielectrophoretically separating live cells from dead cells. Importantly, the system presented can be readily integrated with various external sensors and actuators using commercially available components owing to the device's integration into a PCB.

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Continuous separation of cells by balanced dielectrophoretic forces at multiple frequencies

Cited by 122 publications

References 28 publications

Tracking and synchronization of the yeast cell cycle using dielectrophoretic opacity

Tracking and synchronization of the yeast cell cycle using dielectrophoretic opacity

Investigating dielectric properties of different stages of syngeneic murine ovarian cancer cells

3-D In-Bi-Sn Electrodes for Lab-on-PCB Cell Sorting

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