Continuous production of citric acid from sugarcane molasses using a combination of submerged immobilized and surface stabilized cultures of Aspergillus niger KCU 520

Gupta, Sanjay; Sharma, C.B.

doi:10.1007/bf00128607

Cited by 20 publications

(10 citation statements)

References 16 publications

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“…Many studies have been carried out, mainly on the production of organic acids [1][2][3][4], enzymes [5][6][7] and oligosaccharides [8,9], using mycelia immobilized on various support materials by entrapment or adsorption techniques. With respect to citric acid production, significant studies have been carried out and efforts to achieve higher volumetric productivities under conditions of submerged and surface modes of growth continue [1,[10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Physiological aspects of free and immobilized Aspergillus niger cultures producing citric acid under various glucose concentrations

Papagianni

Mattey

2004

Process Biochemistry

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Section: Introductionmentioning

confidence: 99%

Physiological aspects of free and immobilized Aspergillus niger cultures producing citric acid under various glucose concentrations

Papagianni

Mattey

2004

Process Biochemistry

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“…Submerged fermentation (liquid state bioconversion) has been selected by the world's largest citric acid manufacturers due to the lower capital and laboratory or cost. Other than the utilization of glucose as major substrate, various cheaper substrates such as inulin (Drysdale and McKay 1995), date fruit syrup (Roukas and Kotzekidou 1997), carob pod (Roukas 1998), sugar cane molasses (Gupta 1994) have been used for citric acid production by A. niger. Some other microbes (bacteria and yeast) have been used in the industrial process for citric acid from sugars and n-alkanes (Grewal and Karla 1995;Hossain et al 1984).…”

Section: Introductionmentioning

confidence: 99%

Bioconversion of palm oil mill effluent for citric acid production: statistical optimization of fermentation media and time by central composite design

Alam

Jamal

Nadzir

2007

World J Microbiol Biotechnol

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A laboratory-scale study was conducted to evaluate the feasibility of using palm oil mill effluent (POME) as a major substrate and other nutrients for maximum production of citric acid using the potential fungal strain Aspergillus niger (A103). Statistical optimization of medium composition (substrate-POME, co-substrates-wheat flour and glucose, and nitrogen source-ammonium nitrate) and fermentation time was carried out by central composite design (CCD) to develop a polynomial regression model through the effects of linear, quadratic, and interaction of the factors. The statistical analysis of the results showed that, in the range studied, ammonium nitrate had no significant effect whereas substrate, co-substrates and fermentation time had significant effects on citric acid production. The optimized medium containing 2% (w/w) of substrate concentration (POME), 4% (w/w) of wheat flour concentration, 4% (w/w) of glucose concentration, 0% (w/v) of ammonium nitrate and 5 days fermentation time gave the maximum predicted citric acid of 5.37 g/l which was found to be 1.5 g/l in the experimental run. The determination of coefficient (R 2 ) from the analysis observed was 0.964, indicating a satisfactory adjustment of the model with the response. The analysis showed that the major substrate POME (P \ 0.05), glucose (P \ 0.01), nutrient (P \ 0.05), and fermentation time (P \ 0.01) was more significant for citric acid production. The bioconversion of POME for citric acid production using optimal conditions showed the higher removal of chemical oxygen demand (82%) with the production of citric acid (5.2 g/l) on the final day of fermentation process (7 days). The pH and biosolids accumulation were observed during the bioconversion process.

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“…A cost reduction in citric acid production can be achieved by using cheap agricultural wastes such as apple and grape pomace, orange peel, kiwi fruit peel, cotton waste, okra soy-residue and cane molasses 12 . Other carbohydrates and wastes that have been considered, experimentally, to produce CA by A. niger includes inulin 13 , date fruit syrup 6 , sugar cane molasses 14 , soya whey 15 , kumara 16 and Carob pod 17 and cheese whey 18 . At present time CA is produced commercially using mutant strains of A. niger and with a significant amount by Saccharomycopsis lipolytica 19 , Pencillium simplicissimum 20 and A. foeitidus 21 .…”

Section: Introductionmentioning

confidence: 99%

Production and Partial Characterization of Citric Acid by Local Isolate of Aspergillus Niger Using Sorghum

Patil¹,

Patil²

2014

Int. Res. J. Pharm.

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In the present study, the citric acid producing organisms were isolated from soil sample using serial dilution technique and the isolate were identified on the basis of their microscopic and morphological characteristics. The isolated fungal species were screened on the citric acid production using Czapek-Dox agar medium. From the isolates, fungal species Aspergillus niger were used for citric acid production, and the fungal species subculture using potato dextrose medium. The fresh culture of isolated Aspergillus niger were mixed to the fermentative medium along with the substrates, sorghum flour, corn starch and wheat flour separately and the fermentative medium was incubated 6-7 days at 30 0 C, after incubation the citric acid content was estimated by titrimetric method. Among the three substrates highest citric acid production was observed in corn starch supplemented medium than sorghum and wheat flour medium. Further characterization of citric acid was by paper chromatography, thin layer chromatography and IR. So it is highly recommended that the usage of corn starch supplemented medium with Aspergillus niger will be effective in the production of citric acid than the other substrates.

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Continuous production of citric acid from sugarcane molasses using a combination of submerged immobilized and surface stabilized cultures of Aspergillus niger KCU 520

Cited by 20 publications

References 16 publications

Physiological aspects of free and immobilized Aspergillus niger cultures producing citric acid under various glucose concentrations

Physiological aspects of free and immobilized Aspergillus niger cultures producing citric acid under various glucose concentrations

Bioconversion of palm oil mill effluent for citric acid production: statistical optimization of fermentation media and time by central composite design

Production and Partial Characterization of Citric Acid by Local Isolate of Aspergillus Niger Using Sorghum

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