Physiological aspects of free and immobilized Aspergillus niger cultures producing citric acid under various glucose concentrations

Papagianni, Maria; Mattey, Michael

doi:10.1016/j.procbio.2003.09.027

Cited by 29 publications

(23 citation statements)

References 44 publications

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“…However, Villena and Gutiérrez-Correa [16] reported that free mycelium cultures produced more biomass than biofilm cultures both in shaken flasks as well as in a specially designed microbioreactor. This observation was opposite to the previous three reports [29][30][31] but was in concurrence with the authors' earlier findings [32] and with Papagianni and Mattey [33]. Enhanced levels of supernatant polysaccharides in comparison with protein (by 120-fold for C. crispatum and by 8-fold for G. viride) were associated with biofilm formation on PMMA surfaces by the filamentous fungi of this study, indicating that polysaccharides played an important role than proteins in the formation of the biofilm.…”

Section: Discussionsupporting

confidence: 66%

Cellulase and xylanase activity in relation to biofilm formation by two intertidal filamentous fungi in a novel polymethylmethacrylate conico-cylindrical flask

Mitra

Banerjee

Gachhui

et al. 2011

Bioprocess Biosyst Eng

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A polymethylmethacrylate (PMMA) conico-cylindrical flask (CCF) with an inner arrangement consisting of eight equidistantly spaced rectangular strips mounted radially on a circular disk to provide additional surface area for fungal attachment was employed for production of cellulase by Chaetomium crispatum and xylanase by Gliocladium viride. The design allowed comparison of production between CCFs with hydrophobic surface (PMMA-CCF), hydrophilic glass surface (GS-CCF) and 500-ml Erlenmeyer flask (EF). Compared with the EF, endo-β-1,4-glucanase and FPase (filter paper degradation) activities increased from 0.044 to 0.156 and from 0.008 to 0.021 IU/ml, respectively, in the PMMA-CCF, while growth of C. crispatum was higher by at most 1.38-fold compared with the other vessels. Xylanase production in the EF was at most 5.08-fold higher and growth of G. viride was at most 1.52-fold higher compared with the other vessels. Temporal pattern of biofilm development based on two-channel fluorescence detection of extracellular polymeric substances (EPSs) and whole cells in a confocal laser scanning microscope demonstrated increase by 100% in biovolume, 25% in thickness and 62.5% both in substratum coverage and total spreading of C. crispatum biofilm in PMMA-CCF over 6 days. Biovolume of G. viride biofilm in GS-CCF increased by 150% over 4 days while that in PMMA-CCF enhanced by 200% over 2 days. Biofilm thickness in PMMA-CCF was 44% higher compared with GS-CCF and increased by 175% over 2 days. Substratum coverage was 38% higher in GS-CCF compared with PMMA-CCF. Thus, reactor surface area and property, shear forces and biofilm formation influenced enzyme production.

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Section: Discussionsupporting

confidence: 66%

Cellulase and xylanase activity in relation to biofilm formation by two intertidal filamentous fungi in a novel polymethylmethacrylate conico-cylindrical flask

Mitra

Banerjee

Gachhui

et al. 2011

Bioprocess Biosyst Eng

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“…Interestingly, this group led by Akihiko Sakurai used the term biofilm to refer to the attached biomass of A. niger on a support though the concept was of immobilization. Papagianni and Mattey [172] found that attached cultures of A. niger on pieces of nylon mesh with a pore size of 50 μm in submerged fermentation had significant higher citric acid specific activities than free-living pellets and that this difference may be due to the mean diffusion path in the two systems. Sheets of cellulose microfibers were used as support for A. niger attachment in citric acid production; these were rolled into a spiral with a thin polythene mesh in between the folds to avoid mycelial adhesion between two adjacent layers, and loaded into a reactor which was operated in recycled fed-batch or continuous conditions.…”

Section: Metabolite and Enzyme Production Of Aspergillus Biofilmsmentioning

confidence: 99%

Recent Advances on Filamentous Fungal Biofilms for Industrial Uses

Gutiérrez-Correa

Ludeña

Ramage

et al. 2012

Appl Biochem Biotechnol

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Industrial enzymes are produced by submerged fermentation (SF) and by solid-state fermentation (SSF) to a lesser extent. Although SSF has several advantages, its scale-up is difficult. The role of physiological and genetic properties of microorganisms growing attached to surfaces could explain the advantages of SSF. Filamentous fungi are naturally adapted to growth on surfaces and in these conditions they show a particular physiological behavior which is different from that in SF; thus, they also form biofilms. Fermentation by filamentous fungal biofilms (FFB) is a homogeneous production system within a liquid environment based on the infrastructure of the SF process with the productive efficiency of the SSF. Enzyme production levels of FFB are much higher than those obtained in SF and they are also amenable of mixed fungal cultivation. Transcriptomic and proteomic tools are used to uncover the fundamental biological issues behind FFB. Several genes encoding cellulolytic enzymes are either differentially expressed or overexpressed in FFB. Moreover, our proteomic studies of Aspergillus niger biofilms compared to SF indicate that many intracellular proteins are either differentially expressed or overexpressed. Clinically important fungi like A. fumigatus also form biofilms when they infect lungs and recent studies demonstrate same gene expression features. These results support our hypothesis of cell adhesion and its role in the new schemes for improved fermentative production of industrial enzymes.

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“…The use of immobilized cell systems is considered a more promissory and advantageous alternative when compared to free cell systems, for several reasons as follows: (i) It promotes the increase in biomass concentration that leads to an improvement in the process efficiency and productivity [13]; (ii) viscosity problems caused by certain fungi morphologies, responsible for mass transfer limitations, are eliminated [14]; (iii) it makes easier the biomass reutilization and separation from the fermented broth [15,16]. There are several immobilization techniques, and the right choice of the immobilization method and the type of carrier are crucial factors for the development of an efficient system [8,17].…”

Section: Introductionmentioning

confidence: 99%

Production of fructooligosaccharides and β-fructofuranosidase by batch and repeated batch fermentation with immobilized cells of Penicillium expansum

Mussatto

Prata

Rodrigues

et al. 2012

Eur Food Res Technol

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The production of fructooligosaccharides (FOS) and b-fructofuranosidase (FFase) by immobilized cells of Penicillium expansum was evaluated. In an initial stage, different low-cost materials including synthetic fiber, polyurethane foam, stainless steel sponge, loofah sponge, and cork oak were tested as carrier for the fungus immobilization. Additionally, the influence of the inoculum age (1 or 3 weeks) on cells immobilization, FOS and FFase production was also verified. Synthetic fiber and polyurethane foam were the best materials for P. expansum immobilization (2.21 and 1.98 g/g carrier, respectively) and FOS production (120.3 and 104.8 g/l), and gave also high results of FFase activity (23.01 and 32.42 U/ml). Then, the production of FOS and FFase by repeated batch fermentation with P. expansum immobilized on synthetic fiber was studied, aiming to improve the batch fermentation results. The results obtained in this stage were very promising with FOS yields of 87, 72, and 44 %, in the 3 initial cycles (60 h), respectively; the FFase activity was constant throughout the process (6 cycles, 96 h). Repeated batch fermentation with immobilized cells of P. expansum was found as being a technology with great potential for FOS and FFase production on industrial scale.

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Physiological aspects of free and immobilized Aspergillus niger cultures producing citric acid under various glucose concentrations

Cited by 29 publications

References 44 publications

Cellulase and xylanase activity in relation to biofilm formation by two intertidal filamentous fungi in a novel polymethylmethacrylate conico-cylindrical flask

Cellulase and xylanase activity in relation to biofilm formation by two intertidal filamentous fungi in a novel polymethylmethacrylate conico-cylindrical flask

Recent Advances on Filamentous Fungal Biofilms for Industrial Uses

Production of fructooligosaccharides and β-fructofuranosidase by batch and repeated batch fermentation with immobilized cells of Penicillium expansum

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