Continuous Plasma Separation Form Whole Blood Using Microchannel Geometry

Park, J.; Cho, K.; Chung, Chong-Pyoung; Han, Dao-Man; Chang, Jun Keun

doi:10.1109/mmb.2005.1548368

Cited by 5 publications

(6 citation statements)

References 4 publications

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“…Given the significant relevance of the plasma/blood separation and the current interest in miniaturized and integrated bioanalytical systems, several efforts on the development of microchips for plasma separation and their reviews have been reported [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] previously. An example is the partial separation of plasma from blood in a microchannel flow by applying a standing acoustic wave [5,6], leading to the enrichment of red blood cells (RBCs) in the middle of the microchannel.…”

Section: Introductionmentioning

confidence: 99%

“…An example is the partial separation of plasma from blood in a microchannel flow by applying a standing acoustic wave [5,6], leading to the enrichment of red blood cells (RBCs) in the middle of the microchannel. Alternatively, RBCs were separated from plasma using bifurcation geometry [7][8][9][10] with either a blood-skimming effect [7] or a Zweifach-Fung effect [8][9][10]. Unique microfluidic architectures such as high-aspect-ratio inverted T-shaped microchannel structure [7], Y-shaped microchannel with different flow resistance on the split downstream microchannel [8], or narrow branched microchannel structures [9,10] have been reported.…”

Section: Introductionmentioning

confidence: 99%

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A continuous flow micro filtration device for plasma/blood separation using submicron vertical pillar gap structures

Kang

Yoon

et al. 2014

J. Micromech. Microeng.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A continuous flow micro filtration device for plasma/blood separation using submicron vertical pillar gap structures

Kang

Yoon

et al. 2014

J. Micromech. Microeng.

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“…However, the thickness of the cell-free layer is too insignificant to extract cell-free plasma from the whole blood sample (27). The structure of bifurcation was, therefore, employed to separate plasma and blood cells in the present study.…”

Section: Physical Model and Design Of Microfluidic Channelsmentioning

confidence: 99%

“…Basically, the compact disk (CD)-like platform (20)(21)(22)(23)(24) integrates a disposable disk, containing manifolds with microfluidic functions, and a permanent motor plate to provide centrifugal pumping through spinning. However, the simplest approach is to design microfluidic channels for separating blood cells from plasma (17,(25)(26)(27). The hydrodynamic mechanisms employed in these microfluidic chips are usually the bifurcation law, or plasma-skimming effect (28,29), and the centrifugal force induced by microchannel design.…”

Section: Introductionmentioning

confidence: 99%

Design and Simulation of a Microfluidic Blood-Plasma Separation Chip Using Microchannel Structures

Huang

Pai

et al. 2009

Separation Science and Technology

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Current clinical methods for the separation of whole blood into blood cells and cell-free plasma are currently based on large facility equipment, such as centrifuges. The disadvantage of this process is that the patients must have assays performed at the hospital or laboratory where the separation facility is located. The present study presents a design for microfluidic chips with different microchannel structures, which utilizes backward facing step geometry and centrifugal force to extract the cell-free plasma from whole blood samples at the branch of the microchannel for further assay, avoiding the influence of blood cells. Numerical simulation was performed on a personal computer to analyze the effects of inlet velocity and the structures of the microchannel on the flow field and back-flow in the microchannel, as well as the efficiency of separation and the volumetric fraction of the flowrate of plasma extraction. The minimum radius of particles (R) that can be excluded from the side channel, and fraction of the volumetric flowrate were obtained to evaluate the efficiency of plasma extraction. Based on the numerical simulations, the design with both converging and bending channels was the best design among the four layouts proposed. In this design, the value of R could be set to less than the critical value (set as 1 mm because of the radius of platelets), and the volumetric fraction of the extraction flowrate was approximately 8.4% when Re was about 20. The preliminary experiments indicated the fluorescent particles with 2.5 mm in radius were successfully excluded from side (plasma outlet) channel of the microfluidic chip with converging a inlet channel and the bent microchannel, when the Reynolds number of the inlet flowrate equals 50.

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“…Furthermore, there are high flow resistance, low flow rate and clogging in filter-type microfluidic devices. Therefore, the most simple approach is to design microfluidic channels for separating blood cells from plasma [3][4][5] . The hydrodynamic mechanisms employed in these microfluidic chips are usually the bifurcation law, or plasma-skimming effect 6,7 , and centrifugal force induced by microchannel design.…”

Section: Introductionmentioning

confidence: 99%

Particle-Free Extraction by Using Microchannel Structures

Leu

Pai

2012

Int. J. Mod. Phys. Conf. Ser.

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Modern separation methods of particles are usually prepared by large equipments. In this study, microfluidic chips with backward-facing-step (BFS) microchannel structures and centrifugal force are used to extract particle-free fluid from physical samples at the branch. Numerical simulation and experimental studies were performed to investigate the effects of inlet Reynolds number ( Re 0), as well as the particle-free fluid outlet Reynolds number ( Re 1), on the minimum radius of particles (R) that can be excluded from the particle-free fluid outlet channel. The fraction of the volumetric flow rate of particle-free extraction α (=extraction flow rate/inlet flow rate) was also obtained to evaluate the efficiency of particle-free extraction. Based on the numerical and experimental results, it is found that the design with 90° elbow inlet channel has a better performance than straight inlet channel. In this experiment, 1.0 μm radius of particles can be successfully separated from the fluid, and the volumetric fraction of the extraction flow rate was approximately 1.8% when inlet and outlet Reynolds numbers are 90 and 3.0 respectively.

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Continuous Plasma Separation Form Whole Blood Using Microchannel Geometry

Cited by 5 publications

References 4 publications

A continuous flow micro filtration device for plasma/blood separation using submicron vertical pillar gap structures

A continuous flow micro filtration device for plasma/blood separation using submicron vertical pillar gap structures

Design and Simulation of a Microfluidic Blood-Plasma Separation Chip Using Microchannel Structures

Particle-Free Extraction by Using Microchannel Structures

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