A continuous flow micro filtration device for plasma/blood separation using submicron vertical pillar gap structures

Kang, Tae Goo; Yoon, Yong‐Jin; Ji, Hongmiao; Lim, Pei Yi; Chen, Yu

doi:10.1088/0960-1317/24/8/087001

Cited by 28 publications

(25 citation statements)

References 17 publications

(34 reference statements)

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“…2 The principle behind that filtration technique was based on the size exclusion of cells, whereby only plasma was allowed to pass between the vertical pillars. The effectiveness of Kang et al's approach in separating blood plasma from whole blood was demonstrated experimentally; it was found to have a filtration efficiency of 99.9% and a plasma collection rate of 0.67 ll min À1 .…”

Section: A Microfiltration Based On Microchannel Obstructionmentioning

confidence: 99%

“…1 Compared to large-scale instruments, microfluidic devices require smaller samples and reagent volumes and provide better separation efficiency due to (1) a high surfaceto-volume ratio, (2) shorter analysis time, and (3) higher sensitivity. Microfluidic devices can even be integrated with other components toward a lab-on-a-chip (LOC).…”

Section: Introductionmentioning

confidence: 99%

“…Microfluidic devices can even be integrated with other components toward a lab-on-a-chip (LOC). Blood analysis [2][3][4][5][6][7][8][9][10][11][12][13] involves separating blood plasma from whole human blood to prevent blood cells from affecting final assay results. The conventional approach for plasma separation involves fitting commercial centrifuge tubes with a filter to separate the blood plasma from the whole human blood; however, the centrifugal forces induced by high speed rotation are difficult to be realized on microfluidic platforms.…”

Section: Introductionmentioning

confidence: 99%

“…Microfiltration is the most common technique used to separate plasma from blood, and microfluidic-based filtration approaches include constructing obstructions within the microchannels, [2][3][4] packing beads to create a microfilter, 5,6 and integrating filters within microfluidic a) Pcchen@mail.ntust.edu.tw. Tel.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of thermoplastic microfiltration chip for the separation of blood plasma from human blood

Chen

Young

2016

Biomicrofluidics

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In this study, we developed a fully thermoplastic microfiltration chip for the separation of blood plasma from human blood. Spiral microchannels were manufactured on a PMMA substrate using a micromilling machine, and a commercial polycarbonate membrane was bonded between two thermoplastic substrates. To achieve an excellent bonding between the commercial membrane and the thermoplastic substrates, we used a two-step injection and curing procedure of UV adhesive into a ring-shaped structure around the microchannel to efficiently prevent leakage during blood filtration. We performed multiple filtration experiments using human blood to compare the influence of three factors on separation efficiency: hematocrit level (40%, 23.2%, and 10.9%), membrane pore size (5 lm, 2 lm, and 1 lm), and flow rate (0.02 ml/min, 0.06 ml/min, 0.1 ml/min). To prevent hemolysis, the pressure within the microchannel was kept below 0.5 bars throughout all filtration experiments. The experimental results clearly demonstrated the following: (1) The proposed microfiltration chip is able to separate white blood cells and red blood cells from whole human blood with a separation efficiency that exceeds 95%; (2) no leakage occurred during any of the experiments, thereby demonstrating the effectiveness of bonding a commercial membrane with a thermoplastic substrate using UV adhesive in a ring-shaped structure; (3) separation efficiency can be increased by using a membrane with smaller pore size, by using diluted blood with lower hematocrit, or by injecting blood into the microfiltration chip at a lower flow rate. Published by AIP Publishing. [http://dx

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Section: A Microfiltration Based On Microchannel Obstructionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of thermoplastic microfiltration chip for the separation of blood plasma from human blood

Chen

Young

2016

Biomicrofluidics

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“…In these geometries, the size of the geometries determines the cells deformation [4]. Many literature studies use these structures (micropillars arrays and micro-weir filters), which create a cross-flow filtration to overcome some issues related with clogging and jamming [5,6]. However, for the best of the authors knowledge, the use of such structures was never used for separation and, at the same time, for measuring the deformation index of RBCs.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of Image Processing Methods for the Assessment of the Red Blood Cells Deformability in a Microfluidic Device

et al. 2017

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Abstract. Red blood cells (RBCs) deformability is a high relevant mechanical property, whose variations are associated with some diseases, such as diabetes and malaria. Therefore, the present study aims to compare different image processing methods for assessing the RBCs deformability in a continuous flow, measured in a polydimethylsiloxane (PDMS) microchannel composed by 15 lm spacing inner pillars. The images were acquired with a high speed camera and analyzed with ImageJ software for tracking and measuring the RBCs deformation index (DI). Additionally, to understand the performance of the software, it was performed a comparison between different image processing tools provided by ImageJ and the best methods for the deformation measurements were selected, considering the measured RBCs number and their DIs. The results show that those image methods significantly affect the number of measured RBCs and their DIs and, therefore, the studies focused on the deformability measurements need to take into account the effect of the image processing methods for avoiding loss of relevant information in the images.

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Microfluidic Label‐Free Hydrodynamic Separation of Blood Cells: Recent Developments and Future Perspectives

Lee

Kim

Yang

2023

Adv Materials Technologies

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The biophysical characteristics and counts of blood cells provide useful information regarding pathological conditions. Therefore, the separation of blood components is a crucial task in biology, clinical diagnosis, therapeutics, and personalized medicine. Recently, microfluidics has gained significant interest as an effective technology for separating target cells from heterogeneous cell populations. Within cellular-scale microchannels, cells of interest are separated based on intrinsic properties, such as size, shape, and deformability, followed by further downstream or off-chip analysis. In this review, microfluidic-based hydrodynamic cell-separation technologies used in label-free approaches by categorizing them according to their key working principles are discussed: i) flow fractionation, ii) deterministic lateral displacement, iii) hydrophoresis, iv) inertial migration, and v) microfiltration. An overview of the major separation mechanisms is provided, and the relative performances and features of these technologies are thoroughly discussed. In addition, future perspectives regarding microfluidic system commercialization and standardization are briefly provided for their widespread use and applications.

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A continuous flow micro filtration device for plasma/blood separation using submicron vertical pillar gap structures

Abstract: A continuous flow micro filtration device for plasma/blood separation using submicron vertical pillar gap structures

Cited by 28 publications

References 17 publications

Characterization of thermoplastic microfiltration chip for the separation of blood plasma from human blood

Characterization of thermoplastic microfiltration chip for the separation of blood plasma from human blood

A Comparative Study of Image Processing Methods for the Assessment of the Red Blood Cells Deformability in a Microfluidic Device

Microfluidic Label‐Free Hydrodynamic Separation of Blood Cells: Recent Developments and Future Perspectives

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