Continuous Live-Cell Culture Monitoring by Compact Lensless LED Microscopes

Scholz, Gregor; Mariana, Shinta; Syamsu, Iqbal; Dharmawan, Agus Budi; Schulze, Torben; Mattern, K.L.; Hörmann, Philipp; Hartmann, Jana; Dietzel, Andreas; Rustenbeck, Ingo; Hiller, Karsten; Prades, Joan Daniel; Waag, A.; Wasisto, Hutomo Suryo

doi:10.3390/proceedings2130877

Cited by 3 publications

(2 citation statements)

References 5 publications

(5 reference statements)

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“…With its advantages such as wide field of view, high spatial resolution, easy integration, and low cost, DIHM has become a tool that performs essential functions, especially in the laboratory and clinical stages of medical imaging. DIHM is used in laboratory conditions to imaging microorganisms such as cancer cells, bacteria, yeast cells, or sperm cells, perform viability analyses, track the cells in 2D, and determine sample 3D localizations [38][39][40][41][42]. It is used in the clinic for human-level counting and classifying blood cells, morphological examination of medical samples, and disease diagnosis [43][44][45].…”

Section: Lensless Digital In-line Holographic Microscopymentioning

confidence: 99%

DWT‐SVD Based Watermarking for High‐Resolution Medical Holographic Images

et al. 2022

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Watermarking is one of the most common techniques used to protect data’s authenticity, integrity, and security. The obfuscation in the frequency domain used in the watermarking method makes the watermarking stronger than the obfuscation in the spatial domain. It occupies an important place in watermarking works in imperceptibility, capacity, and robustness. Finding the optimal location to hide the watermarking is one of the most challenging tasks in these methods and affects the method’s performance. In this article, sample identification information is processed with the method of watermaking on the hiding environment created by using a chaos-based random number generator on biomedical data to provide solutions to problems such as visual attack, identity theft, and information confusion. In order to obtain biomedical data, a lensless digital in-line holographic microscopy (DIHM) setup was designed, and holographic data of human blood and cancer cell lines, which are widely used in the laboratory environment, were obtained. The standard USAF 1951 target was used to evaluate the resolution of our imaging setup. Various QR codes were generated for medical sample identification, and the captured medical data were processed by watermarking it with chaos-based random number generators. A new method using chaos-based discrete wavelet transform (DWT) and singular value decomposition (SVD) has been developed and applied to high-resolution data to eliminate the problem of encrypted data being directly targeted by third-party attacks. The performance of the proposed new watermarking method has been demonstrated by various robustness and invisibility tests. Experimental results showed that the proposed scheme reached an average PSNR value of 564588 dB and SSIM value of 0.9972 against several geometric and destructive attacks, which means that the proposed method does not affect the image quality and also ensures the security of the watermarking information. The results of the proposed method have shown that it can be used efficiently in various fields.

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Section: Lensless Digital In-line Holographic Microscopymentioning

confidence: 99%

DWT‐SVD Based Watermarking for High‐Resolution Medical Holographic Images

et al. 2022

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“…Recently, digital inline-holographic microscopes (DIHM) based on light-emitting diode (LED) illumination have gained attention for providing a small and inexpensive but capable microscopy solution [ 3 , 4 , 5 , 6 ]. Due to their not relying on lenses and essentially consisting only of a light source and an image sensor, they can be built in a very cost effective manner [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy

Scholz

Mariana

Dharmawan

et al. 2019

Sensors

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Continuous cell culture monitoring as a way of investigating growth, proliferation, and kinetics of biological experiments is in high demand. However, commercially available solutions are typically expensive and large in size. Digital inline-holographic microscopes (DIHM) can provide a cost-effective alternative to conventional microscopes, bridging the gap towards live-cell culture imaging. In this work, a DIHM is built from inexpensive components and applied to different cell cultures. The images are reconstructed by computational methods and the data are analyzed with particle detection and tracking methods. Counting of cells as well as movement tracking of living cells is demonstrated, showing the feasibility of using a field-portable DIHM for basic cell culture investigation and bringing about the potential to deeply understand cell motility.

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Beyond solid-state lighting: Miniaturization, hybrid integration, and applications of GaN nano- and micro-LEDs

Wasisto

Prades

Gülink

et al. 2019

Applied Physics Reviews

224

116

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Gallium nitride (GaN) light-emitting-diode (LED) technology has been the revolution in modern lighting. In the last decade, a huge global market of efficient, long-lasting, and ubiquitous white light sources has developed around the inception of the Nobel-prize-winning blue GaN LEDs. Today, GaN optoelectronics is developing beyond solid-state lighting, leading to new and innovative devices, e.g., for microdisplays, being the core technology for future augmented reality and visualization, as well as point light sources for optical excitation in communications, imaging, and sensing. This explosion of applications is driven by two main directions: the ability to produce very small GaN LEDs (micro-LEDs and nano-LEDs) with high efficiency and across large areas, in combination with the possibility to merge optoelectronic-grade GaN micro-LEDs with silicon microelectronics in a hybrid approach. GaN LED technology is now even spreading into the realm of display technology, which has been occupied by organic LEDs and liquid crystal displays for decades. In this review, the technological transition toward GaN micro- and nanodevices beyond lighting is discussed including an up-to-date overview on the state of the art.

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Continuous Live-Cell Culture Monitoring by Compact Lensless LED Microscopes

Cited by 3 publications

References 5 publications

DWT‐SVD Based Watermarking for High‐Resolution Medical Holographic Images

DWT‐SVD Based Watermarking for High‐Resolution Medical Holographic Images

Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy

Beyond solid-state lighting: Miniaturization, hybrid integration, and applications of GaN nano- and micro-LEDs

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