Continuous hydrogen and butyric acid fermentation by immobilized Clostridium tyrobutyricum ATCC 25755: Effects of the glucose concentration and hydraulic retention time

Mitchell, Robert J.; Kim, Ji Seong; Jeon, Byung Seung; Sang, Byoung In

doi:10.1016/j.biortech.2009.05.046

Cited by 45 publications

(28 citation statements)

References 17 publications

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“…Table 4 summarizes the key parameters and results of the most relevant studies. All the previous studies performed using immobilized Clostridium butyricum, either using PEG (Singh et al 2013), alginate bead (Zhao et al 2012), polyurethane foam (Jo et al 2008), polyurethane-activated carbon matrix (Mitchell et al 2009), or acetyl cellulose fiber (Karube et al 1982) demonstrated similar or increased molar hydrogen yield when compared to free-cell systems. There are also indications in literature that Clostridium sp.…”

Section: Hydrogen Yield Obtainedmentioning

confidence: 91%

“…(Ghosh and Hallenbeck 2014), but limited immobilization studies with the objective of producing hydrogen have been carried out with this strain. Some work has been done using immobilizing materials such as polyethylene glycol (Singh et al 2013), calcium alginate beads (Zhao et al 2012), polyurethane-activated carbon matrix (Mitchell et al 2009), ethylene-vinyl acetate copolymer (Lin et al 2009), polyurethane foam (Jo et al 2008), silicone gel (Lin et al 2006), and acetyl cellulose filter (Karube et al 1982). Although in these works hydrogen yield and productivity were improved using immobilized bacteria compared to suspended cells, major issues still remain about the reusability, stability, and regeneration of these immobilizing materials.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

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Improving biohydrogen production using Clostridium beijerinckii immobilized with magnetite nanoparticles

Seelert

Ghosh

Yargeau

2015

Appl Microbiol Biotechnol

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In order to supplement the need for alternative energy resources within the near future, enhancing the production of biohydrogen with immobilized Clostridium beijerinckii NCIMB8052 was investigated. Magnetite nanoparticles were functionalized, with chitosan and alginic acid polyelectrolytes using a layer-by-layer method, to promote bacterial attachment. Cultivating C. beijerinckii with these nanoparticles resulted in a shorter lag growth phase and increased total biohydrogen production within 100-ml, 250-ml and 3.6-L reactors compared with freely suspended organisms. The greatest hydrogen yield was obtained in the 250-ml reactor with a value of 2.1±0.7 mol H 2 /mol glucose, corresponding to substrate conversion and energy conversion efficiencies of 52±18 and 10±3 %, respectively. The hydrogen yields obtained using the immobilized bacteria are comparable to values found in literature. However, to make this process viable, further improvements are required to increase the substrate and energy conversion efficiencies.

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Section: Hydrogen Yield Obtainedmentioning

confidence: 91%

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Improving biohydrogen production using Clostridium beijerinckii immobilized with magnetite nanoparticles

Seelert

Ghosh

Yargeau

2015

Appl Microbiol Biotechnol

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“…By contrast their HPR and both acetate and H 2 yields were 2-fold lower than ours. These different metabolite distributions should be related to the different Clostridium species involved in our and their experiments since high butyrate yields and low acetate yields were also reported by Mitchell et al [29] with another C. tyrobutyricum strain.…”

Section: /35mentioning

confidence: 99%

“…However the low CO 2 content would also be related to CO 2 absorption in the unstirred liquid medium and in the replacement equipment [32]. by Van Ginkel and Logan [41] (at a HRT of 10 h) and Mitchell et al [29] (at a HRT of 16.7 h) in similar loading and physico-chemical conditions. At a GLR higher than 0.88 g/L.h, only a marginal increase of HPR could be observed.…”

Section: /35mentioning

confidence: 99%

“…Zhang et al [27] tested a C. acetobutylicum strain in a trickle bed made of glass beads; Zhu and Yang [28] tested C. tyrobutyricum bacteria immobilized in a coton-fibrous bed bioreactor; Mitchell et al [29] tested the same strain immobilized on porous particles (made of polyurethane and activated carbon) in a stirred-tank bioreactor and Jo et al [30] tested another strain of the same species immobilized on a fixed-bed reactor (polyurethane foam matrix).…”

Section: /35mentioning

confidence: 99%

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Improvement of fermentative biohydrogen production by Clostridium butyricum CWBI1009 in sequenced-batch, horizontal fixed bed and biodisc-like anaerobic reactors with biomass retention

Hiligsmann

Beckers

Masset

et al. 2014

International Journal of Hydrogen Energy

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A horizontal tubular fixed bed bioreactor (HFBR) and an anaerobic biodisc-like reactor (AnBDR) were designed to both fix Clostridium biomass and enable rapid transfer of the hydrogen produced to gas phase in order to decrease the strong effect of H 2 partial pressure and H 2 supersaturation on the performances of Clostridium strains. The highest H 2 production rate (703 mL H 2 /L.h) and yield (302 mL/g glucose consumed i.e. 2.4 mol/mol) with the pure culture were recorded in the AnBDR with 300 mL culture medium (total volume 2.3 L) at pH 5.2 and a glucose loading rate of 2.87 g/L.h. These results are about 2.3 and 1.3-fold higher than those achieved in the same bioreactor with 500 mL liquid medium and with the same glucose consumption rate. Therefore, our experimentations and a short review of the literature reported in this paper emphasize the relevance of performing bioreactors with high L/G transfer.Keywords: Clostridium; hydrogen production; biofilm; bioreactor; pure strain CWBI -ULg 2/35 IntroductionThe fermentative production of hydrogen has drawn increased attention in recent years. This biological process called "dark fermentation" (DF) offers new opportunities to produce "green" energy from various renewable resources and organic wastes [1][2][3]. While significant improvements have been made in development of such alternative H 2 production systems, more technical progress and cost reduction needs to occur for them to compete with current large scale technologies e.g. methane-reforming process. By contrast, for local and smaller scale DF and some other opportunities, biohydrogen production processes would be cost competitive since the feedstocks are available almost anywhere and crucial interest is paid for both energy independence and efficient utilization [4,5]. However, optimization is still needed for DF regarding the bioreactor design, rapid removal and purification of gases, use of cheaper feedstock, genetic and molecular engineering to redirect metabolic pathway [6][7][8][9][10].Moreover, DF is only likely to be viable as an industrial process if integrated with a process that maximizes energy recovery from the fermentation end-products. The traditional methaneproducing anaerobic digestion process is the most promising since about 10 to 30 % more energy could be generated in the two-stage integrated system comparing to a single stage methanogenic process [11]. Besides, very prospective processes to convert acetate from DF spent medium exist such as further biohydrogen production (towards the maximum theoretical yield of 12 mol/mol glucose) by photosynthetic non-sulfur bacteria or direct electricity production in microbial fuel cells [6,11].In the past decades, most studies on biohydrogen production processes dealt with suspended culture systems such as the conventional (dis-)continuous stirred tank reactors (CSTR) since they are relatively simple and easy to operate. These investigations, several times reviewed [5,10,12,13], enabled to optimize number of operating parameters such as the ...

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Adaptive evolution for fast growth on glucose and the effects on the regulation of glucose transport system in Clostridium tyrobutyricum

Jiang

et al. 2011

Biotech & Bioengineering

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Laboratory adaptive evolution of microorganisms offers the possibility of relating acquired mutations to increased fitness of the organism under the conditions used. By combining a fibrous-bed bioreactor, we successfully developed a simple and valuable adaptive evolution strategy in repeated-batch fermentation mode with high initial substrate concentration and evolved Clostridium tyrobutyricum mutant with significantly improved butyric acid volumetric productivity up to 2.25 g/(L h), which is the highest value in batch fermentation reported so far. Further experiments were conducted to pay attention to glucose transport system in consideration of the high glucose consumption rate resulted from evolution. Complete characterization and comparison of the glucose phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) were carried out in the form of toluene-treated cells and cell-free extracts derived from both C. tyrobutyricum wide-type and mutant, while an alternative glucose transport route that requires glucokinase was confirmed by the phenomena of resistance to the glucose analogue 2-deoxyglucose and ATP-dependent glucose phosphorylation. Our results suggest that C. tyrobutyricum mutant is defective in PTS activity and compensates for this defect with enhanced glucokinase activity, resulting in the efficient uptake and consumption of glucose during the whole metabolism.

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Continuous hydrogen and butyric acid fermentation by immobilized Clostridium tyrobutyricum ATCC 25755: Effects of the glucose concentration and hydraulic retention time

Cited by 45 publications

References 17 publications

Improving biohydrogen production using Clostridium beijerinckii immobilized with magnetite nanoparticles

Improving biohydrogen production using Clostridium beijerinckii immobilized with magnetite nanoparticles

Improvement of fermentative biohydrogen production by Clostridium butyricum CWBI1009 in sequenced-batch, horizontal fixed bed and biodisc-like anaerobic reactors with biomass retention

Adaptive evolution for fast growth on glucose and the effects on the regulation of glucose transport system in Clostridium tyrobutyricum

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