Continuous flow valorization of fatty acid waste using silica-immobilized lipases

Abstract

Silica immobilized lipases have been prepared and utilized in the valorization of fatty acid-derived food waste streams under continuous flow conditions. Findings demonstrate that better conversions could be obtained when compared with commercially available immobilized enzymes. † Electronic supplementary information (ESI) available. See

Encapsulated Laccases for the Room‐Temperature Oxidation of Aromatics: Towards Synthetic Low‐Molecular‐Weight Lignins

“…The proposed one-pot encapsulation technique required the addition as pecific amount of tetraethoxysilane (TEOS) to ab uffer solution that contained the enzyme and organic solvents to obtain an optimum silica mineralisation,s imilar to that reported previously. [20] In thesee xperiments, 10 mg of enzyme with an activity of 0.92 Umg À1 was added typicallyt o 2.5-5 mL of 50 mm acetic acidb uffer at pH 4.5. The amount and type of solvent, DMSO, acetonitrile (ACN) or MeOH, and Britton-Robinson and acetic acid buffers (at pH 4-6 adjusted with concentrated NaOH and CH 3 COOH, respectively) were tested to determine the optimum conditions for the bio-silicification step (Tables 1a nd 2).…”

“…capsulation at room temperature in ao ne-pot process using as ilicic acid precursor together with ab uffer solution of the enzyme. [20] With the use of this protocol, an ew family of increasingly stable and active bio-nano-hybrid materials (i.e.,l ipases and metal nanoparticle-lipase hybrids) can be synthesised for various bio-catalytic applications,w hich could have as ignificant impact on future industrial bio-processes. [21] With the aim to expand the scope of the bio-silicification enzyme approach, in this contribution we disclose the design of bio-silicified laccases in the framework of ap orous silica using as imilarly simple and efficient one-pot method targeted at the oxidation of lignin model compounds.…”

Enzymes for food waste remediation and valorisation

“…Recently, Itabaiana et al (2013) studied the effects of the encapsulation of Candida antarctica lipase B in mesoporous silica on the esterification of stearic acid and fatty acid food wastes (FAFW). Recently, Itabaiana et al (2013) studied the effects of the encapsulation of Candida antarctica lipase B in mesoporous silica on the esterification of stearic acid and fatty acid food wastes (FAFW).…”

Formation of Functional Nanobiocatalysts with a Novel and Encouraging Immobilization Approach and Their Versatile Bioanalytical Applications

“…While significantly more enzyme were immobilized enzymes on per volume unit of the NMs and higher mechanical and thermal stability were observed, the increase in activity has still remained limited compared to the free enzyme dissolved in solution [28][29][30][31][32][33][34][35][36].…”

“…Although immobilzied α-amylase were highly stable against reaction temperature and some metal ions used as inhibitors, immobilization allows us to reperatedly use it and showed lower activitiy compared to its free form [20][21][22]. Moreover, in order to strengthen bonds between enzymes and the supports, the crosslinking agents played a crucial role to provide covalent attachment [2,[23][24][25][26][27][28][29][30][31][32][33][34][35][36].…”