Continuous Fermentation for Biopharmaceuticals?

Continuous Cultivation as a Tool Toward the Rational Bioprocess Development With Pichia Pastoris Cell Factory

“…Currently, continuous biomanufacturing processes based on the application of continuous fermentation are being carried out in different industries including ethanol, lactic acid, and numerous biopharmaceutical productions. Mears et al ( 2017 ) discussed some of these examples performed with continuous microbial systems at industrial scale to evaluate the expected wide implementation in biopharmaceutical biomanufacturing processes. Actually, the US FDA has encouraged the development of continuous processing for biopharmaceuticals manufacturing (Croughan et al, 2015 ).…”

“…In this sense, it is worth mentioning that Novo Nordisk implemented the continuous insulin production with the yeast S. cerevisiae since the 1990's (Peebo and Neubauer, 2018 ). And currently, several commercial biopharmaceuticals such as monoclonal antibodies or therapeutic proteins are recombinantly produced in continuous perfusion cultures of mammalian cell lines (Mears et al, 2017 ).…”

Opportunities and challenges in single‐cell protein production using lignocellulosic material

“…At the end of the process, the broth is removed in batch fermentation, and the fermenter is again charged with the same material. In continuous fermentation, all of the nutrients, carbon sources and media are added in together continuously, 47 whereas fed‐batch fermentation is a hybrid of the batch mode and continuous fermentation. In general, the final fermentation broth comprises 1–5% solids, and these are separated, washed, dried and used as they are or mixed with other materials.…”