Continuous cultivation of Streptomyces tendae in different media

Lohr, Dorothee; Buschulte, T. K.; Gilles, E. D.

doi:10.1007/bf00184973

Cited by 10 publications

(3 citation statements)

References 7 publications

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“…To study quantitatively the effect of nutrients on the regulation of antibiotic pathways and to determine the limiting nutrient a synthetic medium, established for S. tendae by Lohr et al (1989) was essential. In contrast to nikkomycins, which have a cytotoxic effect on fungi, the juglomycins are active against some bacteria, for example Bacillus subtilis, Staphylococcus aureus, and Mycobacterium tuberculosis , as well as against the producer strain itself.…”

Section: Introductionmentioning

confidence: 99%

Nutritional control of nikkomycin and juglomycin production by Streptomyces tendae in continuous culture

Hege-Treskatis

King

Wolf

et al. 1992

Appl Microbiol Biotechnol

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Continuous cultures with Streptomyces tendae revealed some interesting facts. In a continuous culture running for more than 2500 h the production of either nikkomycines or juglomycins could be selected by varying the feed composition. Decreasing the phosphate supply in the feed broth from the initial concentration of 2.5 mM to 1.0 mM enhanced the productivity of nikkomycins and decreased the productivity of juglomycins. When switching back to the initial conditions of the experiment after 2000 h nearly the same production behaviour as at the beginning of the fermentation could be observed. This indicated a stable behaviour of the population with regard to nikkomycin productivity. The long continuous fermentation showed the ability of S. tendae Tü 901/8c to produce nikkomycin at a high level for at least 1500 h. In a second continuous culture it was shown that the productivity of the nikkomycins and juglomycins decreased and increased, respectively, with increasing dilution rate. Comparing batch cultures with continuous fermentations, higher juglomycin productivity was found in the latter. These facts indicate that the strain responds to complex interacting physiological controls, by producing either nikkomycins or juglomycins in a higher amount.

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Section: Introductionmentioning

confidence: 99%

Nutritional control of nikkomycin and juglomycin production by Streptomyces tendae in continuous culture

Hege-Treskatis

King

Wolf

et al. 1992

Appl Microbiol Biotechnol

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“…Because phosphate is known to regulate many antibiotic syntheses (Martin Offprint requests to: K.-P. Kuhn 1977), batch fermentations were carried out on a defined salts medium with phosphate as the growth-limiting substrate. These investigations have been described by Lohr et al (1989) and Lohr (1989).…”

Section: Introductionmentioning

confidence: 99%

Modelling and parameter identification for batch fermentations with Streptomyces tendae under phosphate limitation

Mundry

Kuhn

1991

Appl Microbiol Biotechnol

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A simple structured model for the dynamics of phosphate-limited batch fermentations with Streptomyces tendae is presented. The model describes the influence of intracellular phosphate storage upon the growth behaviour of the culture. The development of the model takes into account the possible internal regulatory processes of phosphate metabolism. These complex biochemical pathways are summarized with regard to rate-limiting steps to obtain relatively simple model equations. The model parameters are fitted to the experimental data with an identification programme based on the sequential quadratic programming algorithm. Modifications in this algorithm yield a good performance for this application. With respect to the sensitivity of the model parameters, a feedback on the modelling is given. After several loops of modelling and identification, a model was achieved that fits to a set of batch fermentations. Furthermore the simulations show that RNA measurements of some recent fermentations can be interpreted by the simulated internal state variable and that there is evidence for RNA as an intracellular phosphate reserve.

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“…The culture was cultivated for 48 h at 140 rpm and 27°C on a rotary shaker. From this culture 45 mL was transferred into 2.0-L Erlenmeyer flasks containing 900 mL of a defined medium (Lohr et al, 1989). After 48 h, 2.5 L was used to inoculate the 50-L bioreactor (MBR, Wetzikon, Switzerland).…”

Section: Culture and Growth Conditionsmentioning

confidence: 99%

Morphological characterization of filamentous microorganisms in submerged cultures by on-line digital image analysis and pattern recognition

Treskatis

Orgeldinger

Wolf

et al. 1997

Biotechnol. Bioeng.

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Continuous cultivation of Streptomyces tendae in different media

Cited by 10 publications

References 7 publications

Nutritional control of nikkomycin and juglomycin production by Streptomyces tendae in continuous culture

Nutritional control of nikkomycin and juglomycin production by Streptomyces tendae in continuous culture

Modelling and parameter identification for batch fermentations with Streptomyces tendae under phosphate limitation

Morphological characterization of filamentous microorganisms in submerged cultures by on-line digital image analysis and pattern recognition

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