Continuous counter current extraction, isolation and determination of solanesol in Nicotiana tobacum L. by non-aqueous reversed phase high performance liquid chromatography
“…Increasing the processing time further did not increase the yield and in fact, decrease the yield to 0.65 wt% ( p < 0.001). Similar results were observed in previous studies in which the amount of solanesol extracted was decreased by extending maceration period more than 4 hours [4, 5, 37]. This effect might be due to degradation or complexation of solalnesol in the solution.Fig.…”
Section: Resultssupporting
confidence: 91%
“…Dried tomato leaf (Kumato variety) powder (10 g) was placed in a 250 mL soxhlet system and fitted with 250 mL round bottom flask containing 100 mL hexane and refluxed at 60 °C for 2 h [4]. The extract was concentrated with a rotary evaporator (IKA RV10 Basic) at 60 °C.…”
Section: Methodsmentioning
confidence: 99%
“…The biosynthetic pathway for solanesol is via the 2- C -methyl-derythritol4-phosphate (MEP) pathway in plant plastids [3]. Solanesol is a bioactive compound and a precursor used for the synthesis of high-value compounds including vitamins K analogs and coenzyme Q10 [4, 5, 6]. Both vitamin K and coenzyme Q10 consist of quinone rings joined to an isoprenoid tail.…”
Solanesol, the precursor for the synthesis of coenzyme Q10, is currently recovered from tobacco leaves by conventional extraction techniques that require multiple purification steps and a large amount of organic solvents. We recently identified tomato leaves as an alternative source of solanesol and hypothesized that a high-pressure CO
2
extraction could be used as a clean extraction process. The effect of CO
2
pressure and temperature on the extraction of solanesol was determined to achieve high yield and purity. It was found that solanesol could be extracted efficiently by subcritical CO
2
at 25 °C from tomato leaves. The extract contained 40% solanesol and other active compounds such as vitamin K1. A higher level of purity of 93% was achieved using a secondary purification step. Different conventional methods for solanesol extraction was compared to determine the most efficient technique for production of solanesol from tomato leaf. The highest yield of solanesol was achieved at nearly 1% dry weight with using subcritical CO
2
, which was superior to conventional methods.
“…Increasing the processing time further did not increase the yield and in fact, decrease the yield to 0.65 wt% ( p < 0.001). Similar results were observed in previous studies in which the amount of solanesol extracted was decreased by extending maceration period more than 4 hours [4, 5, 37]. This effect might be due to degradation or complexation of solalnesol in the solution.Fig.…”
Section: Resultssupporting
confidence: 91%
“…Dried tomato leaf (Kumato variety) powder (10 g) was placed in a 250 mL soxhlet system and fitted with 250 mL round bottom flask containing 100 mL hexane and refluxed at 60 °C for 2 h [4]. The extract was concentrated with a rotary evaporator (IKA RV10 Basic) at 60 °C.…”
Section: Methodsmentioning
confidence: 99%
“…The biosynthetic pathway for solanesol is via the 2- C -methyl-derythritol4-phosphate (MEP) pathway in plant plastids [3]. Solanesol is a bioactive compound and a precursor used for the synthesis of high-value compounds including vitamins K analogs and coenzyme Q10 [4, 5, 6]. Both vitamin K and coenzyme Q10 consist of quinone rings joined to an isoprenoid tail.…”
Solanesol, the precursor for the synthesis of coenzyme Q10, is currently recovered from tobacco leaves by conventional extraction techniques that require multiple purification steps and a large amount of organic solvents. We recently identified tomato leaves as an alternative source of solanesol and hypothesized that a high-pressure CO
2
extraction could be used as a clean extraction process. The effect of CO
2
pressure and temperature on the extraction of solanesol was determined to achieve high yield and purity. It was found that solanesol could be extracted efficiently by subcritical CO
2
at 25 °C from tomato leaves. The extract contained 40% solanesol and other active compounds such as vitamin K1. A higher level of purity of 93% was achieved using a secondary purification step. Different conventional methods for solanesol extraction was compared to determine the most efficient technique for production of solanesol from tomato leaf. The highest yield of solanesol was achieved at nearly 1% dry weight with using subcritical CO
2
, which was superior to conventional methods.
“…Animals were treated according to the selected doses of extracts in respected groups. 17 Study design for activity The animals were administered with doses for seven days and parameters were evaluated. 18 Datta, et al…”
Section: Disease Induction and Experimental Designmentioning
S. officinalis has numerous common names. Some of the best known are sage, common sage, garden sage, ABSTRACT Aim and Objective: Alzheimer's disease is progressive neurodegenerative disorder which affects older individuals. It is the most common cause of dementia and it is associated with the presence of senile plaques which are deposition of beta-amyloidal protein in the hippocampus area of the brain. Medicinal plants have played vital role in world health. In spite of the great advances observed in modern medicine in recent decades, plants still make an important contribution to health care. The present study is done to evaluate Salvia officinalis, for in vivo study on Alzheimer's disease induced mice. Materials and Methodology: Memory Enhancing Activity, Conditioned avoidance test, Y-maze spontaneous alternation test, Elevated Plus Maze, Morris Water Maze, Measurement of Locomotor Activity were done to assess memory & cognitive functioning. The isolated brain homogenate is estimated for reduced glutathione content, Acetyl cholinesterase Activity, Superoxide dismutase assay (SOD), Lipid peroxidation assay (TBARS), Glutathione peroxidase assay (GSH-Px) and Histopathology examination of brain was performed and analyzed. Results and Discussion: The elevated level of enzymes and decreased level of tissue antioxidant markers were observed in treatment comparative to piracetam treatment group. While 300 mg/kg extract significantly reduced the elevated levels of the enzymes and also significantly increased the tissue antioxidant levels, while decreased the glutathione levels when compared with the control. Conclusion: The histopathological study confirmed the recovery. The herbal extract (150 and 300 mg/kg) has shown effectiveness against Alzheimer's disease.
“…In the processes of purifying nicotine and solanesol, we avoided using high-cost purification methods or more than one step of chromatography, such as preparative HPLC, Bio-Beads chromatography, Sephadex LH-20 chromatography [19], HCCC [20,28] and their combinations. In our procedure, nicotine was purified by one-step solvent fractionation at pH 10.…”
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