Continuous cell lysis in microfluidics through acoustic and optoelectronic tweezers

Witte, Christian; Kremer, Clemens; Cooper, Jonathan M.; Neale, Steven L.

doi:10.1117/12.2004814

Cited by 5 publications

(6 citation statements)

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“…Integrating several microfluidics approaches may further improve diagnostic capability with improved speed and scale of separation. DEP, for example, could also be coupled with the shape-dependent opto-electric cell lysis [ 47 , 48 , 49 ], or specific chemical lysis [ 50 ], wherein RBCs can be selectively destroyed while preserving trypanosomes, improving their detection. The scale at which instruments exploiting these techniques can be produced is amenable to production of handheld devices with readouts readily achieved within the processing power of modern smartphones.…”

Section: Discussionmentioning

confidence: 99%

Microfluidics-Based Approaches to the Isolation of African Trypanosomes

et al. 2017

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African trypanosomes are responsible for significant levels of disease in both humans and animals. The protozoan parasites are free-living flagellates, usually transmitted by arthropod vectors, including the tsetse fly. In the mammalian host they live in the bloodstream and, in the case of human-infectious species, later invade the central nervous system. Diagnosis of the disease requires the positive identification of parasites in the bloodstream. This can be particularly challenging where parasite numbers are low, as is often the case in peripheral blood. Enriching parasites from body fluids is an important part of the diagnostic pathway. As more is learned about the physicochemical properties of trypanosomes, this information can be exploited through use of different microfluidic-based approaches to isolate the parasites from blood or other fluids. Here, we discuss recent advances in the use of microfluidics to separate trypanosomes from blood and to isolate single trypanosomes for analyses including drug screening.

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Section: Discussionmentioning

confidence: 99%

Microfluidics-Based Approaches to the Isolation of African Trypanosomes

et al. 2017

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“…252 OET was also integrated with acoustic tweezers (ATs) for particle manipulation and cell lysis. 253,254 To integrate the two technologies together, an OET device with the SU8 microfluidic channel was put on top of a surface acoustic wave (SAW) transducer, as shown in Fig. 15(d) and (e).…”

Section: Integration With Other Micromanipulation Technologiesmentioning

confidence: 99%

Optoelectronic tweezers: a versatile toolbox for nano-/micro-manipulation

Zhang

El‐Sayed

et al. 2022

Chem. Soc. Rev.

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“…However, it has not been reported whether this hybrid mechanism is capable of testing cell dielectric parameters without changing the liquid solution. In addition, although the combination of ODEP with acoustic tweezers was reported to perform cell lysis and manipulation [121,122], an isotonic solution is still required for the ODEP part, and the improvement in manipulation performance is not significant. By fabricating a pixelated phototransistor array in a photosensitive material, a phototransistor-based ODEP chip was for the first time reported to directly manipulate cells in a cell culture solution [123].…”

Section: Conclusion and Prospectsmentioning

confidence: 99%

Determination of Dielectric Properties of Cells using AC Electrokinetic-based Microfluidic Platform: A Review of Recent Advances

Yang

Wang

et al. 2020

Micromachines

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Cell dielectric properties, a type of intrinsic property of cells, can be used as electrophysiological biomarkers that offer a label-free way to characterize cell phenotypes and states, purify clinical samples, and identify target cancer cells. Here, we present a review of the determination of cell dielectric properties using alternating current (AC) electrokinetic-based microfluidic mechanisms, including electro-rotation (ROT) and dielectrophoresis (DEP). The review covers theoretically how ROT and DEP work to extract cell dielectric properties. We also dive into the details of differently structured ROT chips, followed by a discussion on the determination of cell dielectric properties and the use of these properties in bio-related applications. Additionally, the review offers a look at the future challenges facing the AC electrokinetic-based microfluidic platform in terms of acquiring cell dielectric parameters. Our conclusion is that this platform will bring biomedical and bioengineering sciences to the next level and ultimately achieve the shift from lab-oriented research to real-world applications.

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Continuous cell lysis in microfluidics through acoustic and optoelectronic tweezers

Cited by 5 publications

References 0 publications

Microfluidics-Based Approaches to the Isolation of African Trypanosomes

Microfluidics-Based Approaches to the Isolation of African Trypanosomes

Optoelectronic tweezers: a versatile toolbox for nano-/micro-manipulation

Determination of Dielectric Properties of Cells using AC Electrokinetic-based Microfluidic Platform: A Review of Recent Advances

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