Continuous biosynthesis of abscisic acid (ABA) may be required for maintaining dormancy of isolated embryos and intact seeds of Euonymus alatus

Thammina, Chandra; He, Mingyue; Hao, Yanming; Chen, Yongqin; Gai, Ying; Cao, Kaishuang; Lu, Litang; Zhao, Degang; Wang, Yuejin; McAvoy, Richard; Ellis, Donna; Li, Yang

doi:10.1007/s11240-011-0063-z

Cited by 4 publications

(4 citation statements)

References 43 publications

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“…Seed dormancy prevents seedling growth under unfavorable environmental conditions 40 , 41 . The germination of mature seeds of E. alatus is also delayed by prolonged seed dormancy 41 .…”

Section: Discussionmentioning

confidence: 99%

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Efficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus

Woo

Jie

et al. 2021

Sci Rep

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To establish an efficient plant regeneration system from cell suspension cultures of Euonymus alatus, embryogenic callus formation from immature embryos was investigated. The highest frequency of embryogenic callus formation reached 50% when the immature zygotic embryos were incubated on Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D). At higher concentrations of 2,4-D (over 2 mg/L), the frequency of embryogenic callus formation declined significantly. The total number of somatic embryos development was highest with the 3% (w/v) sucrose treatment, which was found to be the optimal concentration for somatic embryo formation. Activated charcoal (AC) and 6-benzyladenine (BA) significantly increased the frequency of plantlet conversion from somatic embryos, but gibberellic acid (GA3) had a negative effect on plantlet conversion and subsequent development from somatic embryos. Even though the cell suspension cultures were maintained for more than 1 year, cell aggregates from embryogenic cell suspension cultures were successfully converted into normal somatic embryos with two cotyledons. To our knowledge, this is the first successful report of a plant regeneration system of E. alatus via somatic embryogenesis. Thus, the embryogenic cell line and plant regeneration system established in this study can be applied to mass proliferation and production of pharmaceutical metabolite in E. alatus.

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“…Seed dormancy prevents seedling growth under unfavorable environmental conditions 40 , 41 . The germination of mature seeds of E. alatus is also delayed by prolonged seed dormancy 41 .…”

Section: Discussionmentioning

confidence: 99%

“…Seed dormancy prevents seedling growth under unfavorable environmental conditions 40 , 41 . The germination of mature seeds of E. alatus is also delayed by prolonged seed dormancy 41 . Embryo growth is a critical step for the in vitro development and germination of somatic embryos.…”

Section: Discussionmentioning

confidence: 99%

Efficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus

Woo

Jie

et al. 2021

Sci Rep

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“…Por su parte, Thammina et al (2012) reportaron un incremento de 26% en la germinación de semillas de Euonymus alatus sobre el control, usando una concentración baja de ácido giberélico (0.05 mg100mL -1 ). En nuestro estudio, se confirma que el ácido giberélico ayuda a eliminar la latencia de las semillas de A. hypogaea y proporciona una mayor germinación de ellas.…”

Section: Effect Of Pre-germinative Treatmentsunclassified

“…In this sense, ATRIP and O'Reilly (2007) observed a 15% increase in seed germination of Betula pubescens on control, using gibberellic acid (20 mg 100 mL -1 ). Thammina et al (2012) reported a 26% increase in seed germination of Euonymus alatus on the control, using a low concentration of gibberellic acid (0.05 mg100 mL -1…”

Section: Effect Of Pre-germinative Treatmentsmentioning

confidence: 99%

Efecto de tratamientos pre germinativos e inoculación microbiana en cacahuate (Arachis hypogaea L.)

Ruíz-Sánchez¹,

Canul-Díaz²,

Pacheco-Aguirre³

et al. 2017

Remexca

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ResumenEl cultivo de cacahuate (Arachis hypogaea L.), ampliamente utilizado por los agricultores por su rentabilidad y mejoramiento de las propiedades físicas y químicas edáficas, presenta una prolongada latencia de semillas, que va hasta seis meses. En otras especies vegetales la latencia de semillas se ha tratado con inductores de germinación como el ácido giberélico (AG 3 ), el ácido indolacético y naproxeno (NPX). Otro problema técnico en el cultivo de cacahuate es el crecimiento lento de las primeras etapas de las plantas. Lo anterior podría incrementar con el uso de cepas bacterianas promotoras de crecimiento. El objetivo del presente trabajo fue evaluar el efecto del AG 3 y NPX como inductores de germinación, y cepas de Bacillus spp. como promotoras de crecimiento vegetal. En la germinación se usaron dos concentraciones y dos tiempos de inmersión en AG 3 y NPX, mientras que para mejorar crecimiento se usaron tres cepas de Bacillus spp. (CBTC1, CBCC57 y CBRF12) como inoculantes. La germinación incrementó con el tratamiento de 10 mg mL -1 de AG 3 ; en tanto, la tasa relativa de crecimiento en altura de las plantas aumentó significativamente a los 15 días después de la siembra, cuando se inocularon con la cepa CBRF12 de Bacillus spp. Asimismo, se encontró un mayor crecimiento significativo de la biomasa seca de la raíz a los 30 días luego de la siembra, con la cepa CBTC1. En conclusión, el porcentaje de germinación de A. AbstractPeanut crop (Arachis hypogaea L.) is widely used by farmers for their profitability and improvement of the physical and chemical soil traits has a prolonged seed dormancy, which runs up to six months. In other plant species, seed dormancy has been treated with inducers of germination, such as gibberellic acid (AG 3 ), indoleacetic acid and Naproxen (NPX). Another technical issue in the peanut crop is the slow growth of the early stages of the plants. This would increase with the use of bacterial strains to growth promoters. The aim of this study was to evaluate the effect of AG 3 and NPX as inducers of germination, and strains of Bacillus spp. as promoting plant growth. Germination and two times two concentrations immersion in AG 3 and NPX were used, while three strains of Bacillus spp were used as inoculants to enhance growth (CBTC1, CBCC57 and CBRF12). Germination increased with the treatment of 10 mg mL -1 of AG 3 ; meanwhile, the relative growth rate in height of the plants was significantly increased at 15 days after sowing, when inoculated with the strain of Bacillus spp CBRF12. We also found a significantly larger increase in root dry biomass at 30 days after planting, with the CBTC1 strain. In conclusion, the percentage of germination of A. hypogaeae increased with the addition of AG 3 . Also, the growth of the plant increased, albeit slightly, and other significantly only 1178 Rev. Mex. Cienc. Agríc. Vol.6 Núm. 6 14 de agosto -27 de septiembre, 2015Esaú Ruiz-Sánchez et al. hypogaeae incrementó con la adición de AG 3 . Por su parte, el crecimiento de la planta aumentó, aun...

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ABA and GA4 dynamic modulates secondary dormancy and germination in Syngonanthus verticillatus seeds

Barreto

Herken

Silva

et al. 2020

Planta

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Continuous biosynthesis of abscisic acid (ABA) may be required for maintaining dormancy of isolated embryos and intact seeds of Euonymus alatus

Cited by 4 publications

References 43 publications

Efficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus

Efficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus

Efecto de tratamientos pre germinativos e inoculación microbiana en cacahuate (Arachis hypogaea L.)

ABA and GA4 dynamic modulates secondary dormancy and germination in Syngonanthus verticillatus seeds

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