Continuous biodiesel conversion via enzymatic transesterification catalyzed by immobilized Burkholderia lipase in a packed-bed bioreactor

Tran, Dang Thuan; Chen, Ching Lung; Chang, Jo‐Shu

doi:10.1016/j.apenergy.2016.01.082

Cited by 63 publications

(29 citation statements)

References 46 publications

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“…In another relevant example, multiple continuous packed‐bed columns have been used in the enzymatic transesterification for the production of biodiesel catalyzed by the lipase from Burkholderia sp immobilized in alkyl‐grafted celite . The main objective was to determine the optimal dimensions of the reaction column to prevent the accumulation of glycerol at the bottom of the column (a frequent problem in this type of configuration), and the pressure drop inside the reactor.…”

Section: Solvent‐free Biocatalysismentioning

confidence: 99%

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Continuous Biocatalysis in Environmentally‐Friendly Media: A Triple Synergy for Future Sustainable Processes

Guajardo

Marı́a²

2019

ChemCatChem

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Enzyme catalysis (biocatalysis) is a mature strategy to perform organic synthesis with high selectivity and under mild reaction conditions. However, despite their origin and biodegradability, the simple use of enzymes is not sufficient to validate a process as green or sustainable. To reinforce the value that biocatalysis may offer, enzymatic processes can be established in environmentally‐friendly media, such as water, neoteric solvents (e. g. deep‐eutectic‐solvents, DES), biogenic solvents (e. g. 2‐MeTHF or terpenes), or even solvent‐free systems (if substrates allow that). Furthermore, these options can be conducted in continuous biocatalytic processes, which are more efficient and sustainable. Hence, a triple synergy is generated, and very promising environmentally‐friendly options can be envisaged. This conceptual paper discusses these alternatives, providing relevant, recently reported, successful examples.

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Section: Solvent‐free Biocatalysismentioning

confidence: 99%

“… Production of biodiesel in packed‐bed reactor. (1) Substrates (oil and methanol); (2) Pump; (3) Catalyst bed; (4) decanter for the removal of glycerol by the bottom; (5) methanol supplier …”

Section: Solvent‐free Biocatalysismentioning

confidence: 99%

Continuous Biocatalysis in Environmentally‐Friendly Media: A Triple Synergy for Future Sustainable Processes

Guajardo

Marı́a²

2019

ChemCatChem

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“…The advantage of a continuous reactor over a batch reactor is that there is no need to unload, clean and reload the reactor after each batch. Accumulation of glycerol would not only inhibit the enzymatic reaction, but also increase the mass transfer resistance of substrates to immobilized lipase and may cause column clogging and pressure dropping 124 . An example of PBR was tested by Xu et al 123 The stability of immobilized lipase in term of mechanical and operational determines its suitability to be used in a reactor.…”

Section: Reactor Designmentioning

confidence: 99%

“…An example of PBR was tested by Xu et al 123 The stability of immobilized lipase in term of mechanical and operational determines its suitability to be used in a reactor. In addition, instead of the long single PBR, Tran et al 124 designed a serial PBRs (three columns in series) To avoid lipase deactivation, methanol can be added into the reaction system in a continuous or stepwise manner.…”

Section: Reactor Designmentioning

confidence: 99%

Enzymatic transesterification for biodiesel production: a comprehensive review

et al. 2016

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Biodiesel is a type of renewable fuel and a potential alternative for continuously consumed fossil resources. Currently, method applied for biodiesel production is transesterification which is divided into non-catalyzed reaction, chemical-catalyzed reaction and enzymatic reaction. Enzymatic reaction is more advantageous than the other methods because of its mild reaction conditions, easy product recovery, no wastewater generation, no saponification and higher quality of products. The main component in this reaction is an enzyme called lipase which can catalyze wide variety of substrate including free fatty acids. Two other main raw materials for biodiesel synthesis are oil and acyl acceptor such as alcohol. Biodiesel catalyzed by enzyme is affected by many factors such as lipase specificity, lipase immobilization, oil composition and purity, oil to acyl acceptor molar ratio, acyl acceptors, temperature, and water content. Many methods have been tested to manipulate these factors and improve the enzymatic reaction for biodiesel production. These methods include combination of lipases, enzyme pretreatment, enzyme post treatment, methanol addition technique, use of solvent and silica gel, and reactor design. This paper will critically discuss the three major components of enzymatic production of biodiesel and the methods used to improve enzymatic reaction, as well as a review on its economic evaluation and industrial scale production.

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“…In the case of PBR, the use of solvents is recommended because during the transesterification reaction the glycerol produced can accumulate and cause column clogging, increasing reactor pressure. Furthermore, the glycerol produced may be adsorbed on the surface of the support of lipase immobilization, turning it inaccessible to hydrophobic substrates, which makes hydrophobic supports recommended for this application . The use of solvent reduces these problems, protecting the enzyme and reducing the substrate viscosity, further improving mass transfer rates through the bed .…”

Section: Introductionmentioning

confidence: 99%

Enzymatic synthesis of ethyl esters from waste oil using mixtures of lipases in a plug‐flow packed‐bed continuous reactor

Poppe

Matte

Freitas

et al. 2018

Biotechnology Progress

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This work describes the continuous synthesis of ethyl esters via enzymatic catalysis on a packed-bed continuous reactor, using mixtures of immobilized lipases (combi-lipases) of Candida antarctica (CALB), Thermomyces lanuginosus (TLL), and Rhizomucor miehei (RML). The influence of the addition of glass beads to the reactor bed, evaluation of the use of different solvents, and flow rate on reaction conditions was studied. All experiments were conducted using the best combination of lipases according to the fatty acid composition of the waste oil (combi-lipase composition: 40% of TLL, 35% of CALB, and 25% of RML) and soybean oil (combi-lipase composition: 22.5% of TLL, 50% of CALB, and 27.5% of RML). The best general reaction conditions were found to be using tert-butanol as solvent, and the flow rate of 0.08 mL min . The combi-lipase reactors operating at steady state for over 30 days (720 h), kept conversion yields of ∼50%, with average productivity of 1.94 g h , regardless of the type of oil in use. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:952-959, 2018.

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Continuous biodiesel conversion via enzymatic transesterification catalyzed by immobilized Burkholderia lipase in a packed-bed bioreactor

Cited by 63 publications

References 46 publications

Continuous Biocatalysis in Environmentally‐Friendly Media: A Triple Synergy for Future Sustainable Processes

Continuous Biocatalysis in Environmentally‐Friendly Media: A Triple Synergy for Future Sustainable Processes

Enzymatic transesterification for biodiesel production: a comprehensive review

Enzymatic synthesis of ethyl esters from waste oil using mixtures of lipases in a plug‐flow packed‐bed continuous reactor

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