Continuous biocatalytic synthesis of (R)-2-octanol with integrated product separation

Abstract

The continuous biocatalytic synthesis of (R)-2-octanol carried out in an enzyme membrane reactor showed an excellent process stability of the biocatalysts, resulting in a turnover number of 15 million for the applied alcohol dehydrogenase. Utilisation of the ionic liquid AMMOENG^TM 101 as a feasible cosolvent increased substrate concentration, and improved space time yields and turnover numbers of the cofactor by factors of 3 and 6, respectively. Moreover, 80% less waste was generated, while producing the same amount of product. Integrated product separation was realized via solid phase extraction. Extraction of the applied solid phase with supercritical carbon dioxide allowed more than 30 reuses of the solid phase

Enantioselective reduction of sparingly water-soluble ketones: continuous process and recycle of the aqueous buffer system

“…To determine enantiomeric excess (ee), samples of the n-heptane phase (250 μL) were mixed with N-MSTFA (50 μL), heated to 80 °C for 30 min and analysed by GC. 13…”

“…Especially, NR 4 + Cl − -IL were beneficial for the TON of the cofactor as well as for the space-time-yield (STY) and the reaction rate. 12,13 Biocatalysis is generally regarded as safer, greener and more sustainable than conventional chemical catalysis. 14 This is often credited with the fact that water is used as a solvent.…”

New Generations of Ionic Liquids Applied to Enzymatic Biocatalysis

“…Improved storage stabilities and improved enzyme activities were found in the most promising, ammonium-based, AMMOENG TM 101 IL [32]. Later, the same group [33] proved the feasibility of continuous production using the previously recommended IL, combined with product separation using a membrane bioreactor (the so-called process integration). Hussain and co-workers [34] showed that the use of 10%…”

Co-immobilized Alcohol Dehydrogenase and Glucose Dehydrogenase with Resin Extraction for Continuous Production of Chiral Diaryl Alcohol

“…Using commercially available enzyme membrane reactor, (R)-2-octanol could be continuously synthesized by co-immobilized LbADH and GDH, and produced a fairly high STY of 454 g/(L•d). Additionally, product separation and cofactor recycling were achieved by supercritical carbon dioxide (scCO2) extraction [41] (Table 2).…”