Continuous assessment of metabolic activity of mitochondria using resonance Raman microspectroscopy

Lalonde, Joshua W.; Noojin, Gary D.; Pope, Nathaniel J.; Powell, Samantha M.; Yakovlev, Vladislav V.; Denton, Michael L.

doi:10.1002/jbio.202000384

Cited by 7 publications

(3 citation statements)

References 40 publications

(39 reference statements)

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“…7 Surface enhanced Raman spectroscopy uses plasmonic substrate to create local hot spots of electric field enhancement which ultimately increases the efficiency of Raman scattering processes. 8 Resonance Raman spectroscopy utilizing electronic excitation has been also widely used to increase the strength of the signal and can be readily accomplished with cytochrome c 9 and beta carotene in the visible part of the spectrum, and with a large number of organic molecules in the deep UV regime. 10 The above-mentioned improvements improve the signal strengths; however, there is a continuous quest for even higher SNR.…”

Section: Introductionmentioning

confidence: 99%

Denoising Raman spectra using a single layer convolutional model trained on simulated data

Gil

Cheburkanov

Yakovlev

2023

J Raman Spectroscopy

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Raman spectroscopy is a powerful means of revealing chemical and structural information about a sample and acquiring chemically specific images. Such images often suffer from low signal to noise ratios (SNR). In this report, a novel way to improve the SNR using machine learning tools based on simulated data. The proposed approach offers an alternative to time consuming acquisition and labeling of large data sets and can be readily applied to unknown systems. Here, the efficacy of a single layer denoising network trained only on simulated data was evaluated, and it was found that the proposed model was able to provide a substantial improvement in SNR.

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Section: Introductionmentioning

confidence: 99%

Denoising Raman spectra using a single layer convolutional model trained on simulated data

Gil

Cheburkanov

Yakovlev

2023

J Raman Spectroscopy

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“…4 Although many of the features of PBM occur at the "downstream" physiological level, with blood flow and potential amplification via extracellular signaling pathways, we have investigated the detection of immediate effects of photon interaction using in vitro systems. [15][16][17][18][19] Characterizing the fundamental light effects on cells and mitochondria, using rigorous biophysical and enzyme activity methods, can provide valuable information on the initial mechanisms for PBM. It is hoped that knowledge of initial mechanisms of PBM can inform dosimetry requirements for successful induction of PBM therapy in animals and humans that can be translated to enhancing performance.…”

Section: Introductionmentioning

confidence: 99%

Assessment of NIR and THz photostimulation at the cellular and subcellular levels

Denton

Noojin

Pope

2023

Mechanisms of Photobiomodulation Therapy XVII

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The term photobiomodulation (PBM), where modulation usually implies a positive or negative response, has been used to describe a photostimulation effect on cells and tissues that leads to beneficial physiological outcomes, such as increased cellular energetics (ATP) and faster healing. Literature points to multiple hypotheses for the mechanism of PBM therapy when using light in the wavelength range of red to near infrared (NIR). We have used a variety of methods and experimental systems to measure light-dependent biological effects in response to laser parameters that would be expected to produce the physiological effects associated with PBM. Here, we highlight two in vitro experiments addressing two different hypotheses for the mechanism of PBM initiation. First, based on wavelength-dependent efficiencies for nitric oxide (NO) release in hTERT-RPE1 cells, we have used these dependences to determine if optimal NO release correlates to increased activity of complex C-IV in the electron transport chain (ETC). Second, to find any indication that water vibration can lead to stimulation of C-IV activity, we exposed isolated mitochondria with laser light at a frequency of 2.52 terahertz (THz) and compared the results with photostimulation of C-IV activity at 808 nm.

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“…The excitation wavelength of RRS is usually located in the ultraviolet-visible absorption region, making the laser wavelength equal or close to the absorption peak of the material, which will greatly increase the intensity of Raman signal, and thus lower the limit of detection and reduce the measurement time, making it suitable for the detection of some biological samples. Several studies have reported that RRS could be used to detect mitochondrial function in both isolated cells and tissues [15,16].…”

Section: Introductionmentioning

confidence: 99%

Label-free Assessment of Vericiguat Therapy on Mitochondrial Redox States in Septic Mice by Resonance Raman Spectroscopy

Zhao,

Yang,

Zheng

et al. 2023

PIER Letters

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Sepsis is a life-threatening infectious disease. Mitochondrial dysfunction is widespread in severe sepsis. The myocardium contains a large number of mitochondria, and the survival rate of sepsis decreases sharply when cardiac dysfunction is involved. Vericiguat (BAY 1021189) is a novel drug for the prevention of heart failure. In this study, we evaluated the mitochondrial function of septic mice and drug-treated mice by resonance Raman spectroscopy (RRS). RRS is a non-invasive and label-free technique that can identify molecular vibrations by their unique fingerprints, making it ideal for quantitative studies. By choosing 532 nm as the excitation wavelength, which is close to the absorption peak of cytochrome, we can greatly enhance the Raman signal of mitochondrial redox state. RRS can accurately identify the Raman characteristic peak at 750 cm −1 , 1128 cm −1 and 1585 cm −1 attributed to the reduced cytochrome in septic mice. We found that the intensity of the characteristic peak was significantly decreased in septic mice, indicating an imbalance of mitochondrial redox function, while the function was improved in the drug-treated group. It proves that BAY has the potential as a novel treatment for mitochondrial dysfunction in sepsis.

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Continuous assessment of metabolic activity of mitochondria using resonance Raman microspectroscopy

Cited by 7 publications

References 40 publications

Denoising Raman spectra using a single layer convolutional model trained on simulated data

Denoising Raman spectra using a single layer convolutional model trained on simulated data

Assessment of NIR and THz photostimulation at the cellular and subcellular levels

Label-free Assessment of Vericiguat Therapy on Mitochondrial Redox States in Septic Mice by Resonance Raman Spectroscopy

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