Contextual Pavlovian conditioning in the crab Chasmagnathus

Fustiñana, María Sol; Carbó-Tano, Martin; Romano, Arturo; Pedreira, Marı́a Eugenia

doi:10.1007/s10071-012-0570-2

Cited by 22 publications

(49 citation statements)

References 44 publications

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“…To further explore this issue, we initially studied the effect of UPS inhibition on long-term memory in contextual conditioning of the crab Chasmagnathus. In this task, a context (conditioned stimulus, CS) was presented with a cofinishing unconditioned stimulus (US), a visual danger stimulus (Fustiñana et al 2013). We administered the proteasome inhibitor MG132 at a 2.38 mg/animal dose or dimethyl sulfoxide as vehicle (VEH) systemically before training (15-trials) to either trained animals (TR group) or control animals that did not receive the danger stimulus (CT group).…”

Section: Resultsmentioning

confidence: 99%

“…In contextual conditioning of Chasmagnathus, memory retrieval is induced by a brief re-exposure of the animals to the CS without US presentation (Fustiñana et al 2013). The retrieval session consisted of 27 sec of re-exposure to the CS with 5 min of total re-exposure to the experimental device.…”

Section: Inhibition Of the Ups During Reconsolidation Does Not Impairmentioning

confidence: 99%

“…The retrieval session consisted of 27 sec of re-exposure to the CS with 5 min of total re-exposure to the experimental device. Under these conditions, memory is labilized and the reconsolidation process is induced (Fustiñana et al 2013). We trained two pairs of TR and CT groups, and 24 h later one CT-TR pair was injected with MG132 at the same dose used in the previous experiment and the other CT-TR pair was injected with vehicle (four groups: CT-VEH, TR-VEH, CT-MG132, and TR-MG132).…”

Section: Inhibition Of the Ups During Reconsolidation Does Not Impairmentioning

confidence: 99%

“…This drug was extensively used for memory disruption in both consolidation and reconsolidation (Merlo et al 2002Freudenthal et al 2005;Boccia et al 2007;Lubin and Sweatt 2007;Si et al 2012). Recently, we found that sulfasalazine impaired crab contextual memory specifically when it was reactivated by presentation of the training context without reinforcement (Fustiñana et al 2013). To test whether the proteasome inhibitor MG132 is able to block this memory impairment of sulfasalazine in reconsolidation, we trained three matched pairs of TR and CT groups.…”

Section: Inhibition Of the Ups During Reconsolidation Blocks The Amnementioning

confidence: 99%

“…Bicuculline has been found to induce memory enhancement in different species and types of learning (Chapouthiers and Venault 2002). In particular, bicuculline caused enhancement of consolidation and reconsolidation in the crab (Carbo Tano et al 2009, Fustiñana et al 2013. During training session on Day 1, animals received a weak training protocol of four trials, which does not lead to memory retention.…”

Section: Inhibition Of the Ups Blocks The Action Of Mk801 And Bicuculmentioning

confidence: 99%

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Protein degradation by ubiquitin–proteasome system in formation and labilization of contextual conditioning memory

et al. 2014

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The ubiquitin -proteasome system (UPS) of protein degradation has been evaluated in different forms of neural plasticity and memory. The role of UPS in such processes is controversial. Several results support the idea that the activation of this system in memory consolidation is necessary to overcome negative constrains for plasticity. In this case, the inhibition of the UPS during consolidation impairs memory. Similar results were reported for memory reconsolidation. However, in other cases, the inhibition of UPS had no effect on memory consolidation and reconsolidation but impedes the amnesic action of protein synthesis inhibition after retrieval. The last finding suggests a specific action of the UPS inhibitor on memory labilization. However, another interpretation is possible in terms of the synthesis/degradation balance of positive and negative elements in neural plasticity, as was found in the case of long-term potentiation. To evaluate these alternative interpretations, other reconsolidation-interfering drugs than translation inhibitors should be tested. Here we analyzed initially the UPS inhibitor effect in contextual conditioning in crabs. We found that UPS inhibition during consolidation impaired long-term memory. In contrast, UPS inhibition did not affect memory reconsolidation after contextual retrieval but, in fact, impeded memory labilization, blocking the action of drugs that does not affect directly the protein synthesis. To extend these finding to vertebrates, we performed similar experiments in contextual fear memory in mice. We found that the UPS inhibitor in hippocampus affected memory consolidation and blocked memory labilization after retrieval. These findings exclude alternative interpretations to the requirement of UPS in memory labilization and give evidence of this mechanism in both vertebrates and invertebrates.

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Section: Resultsmentioning

confidence: 99%

Section: Inhibition Of the Ups During Reconsolidation Does Not Impairmentioning

confidence: 99%

Section: Inhibition Of the Ups During Reconsolidation Does Not Impairmentioning

confidence: 99%

Section: Inhibition Of the Ups During Reconsolidation Blocks The Amnementioning

confidence: 99%

Section: Inhibition Of the Ups Blocks The Action Of Mk801 And Bicuculmentioning

confidence: 99%

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Protein degradation by ubiquitin–proteasome system in formation and labilization of contextual conditioning memory

et al. 2014

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A crabs' high‐order brain center resolved as a mushroom body‐like structure

Maza

Sztarker

Cozzarin

et al. 2020

J of Comparative Neurology

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The hypothesis of a common origin for high-order memory centers in bilateral animals presents the question of how different brain structures, such as the vertebrate hippocampus and the arthropod mushroom bodies, are both structurally and functionally comparable. Obtaining evidence to support the hypothesis that crustaceans possess structures equivalent to the mushroom bodies that play a role in associative memories has proved challenging. Structural evidence supports that the hemiellipsoid bodies of hermit crabs, crayfish and lobsters, spiny lobsters, and shrimps are homologous to insect mushroom bodies. Although a preliminary description and functional evidence supporting such homology in true crabs (Brachyura) has recently been shown, other authors consider the identification of a possible mushroom body homolog in Brachyura as problematic. Here we present morphological and immunohistochemical data in Neohelice granulata supporting that crabs possess well-developed hemiellipsoid bodies that are resolved as mushroom bodies-like structures. Neohelice exhibits a peduncle-like tract, from which processes project into proximal and distal domains with different neuronal specializations. The proximal domains exhibit spines and en passant-like processes and are proposed here as regions mainly receiving inputs. The distal domains exhibit a "trauben"-like compartmentalized structure with bulky terminal specializations and are proposed here as output regions. In addition, we found microglomeruli-like complexes, adult neurogenesis, aminergic innervation, and elevated expression of proteins necessary for memory processes. Finally, in vivo calcium imaging suggests that, as in insect mushroom bodies, the output regions exhibit stimulus-specific activity. Our results support the shared organization of memory centers across crustaceans and insects.

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Invertebrate learning and cognition: relating phenomena to neural substrate

Perry

Barron

Chen

2013

WIRES Cognitive Science

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Diverse invertebrate species have been used for studies of learning and comparative cognition. Although we have gained invaluable information from this, in this study we argue that our approach to comparative learning research is rather deficient. Generally invertebrate learning research has focused mainly on arthropods, and most of that within the Hymenoptera and Diptera. Any true comparative analysis of the distribution of comparative cognitive abilities across phyla is hampered by this bias, and more fundamentally by a reporting bias toward positive results. To understand the limits of learning and cognition for a species, knowing what animals cannot do is at least as important as reporting what they can. Finally, much more effort needs to be focused on the neurobiological analysis of different types of learning to truly understand the differences and similarities of learning types. In this review, we first give a brief overview of the various forms of learning in invertebrates. We also suggest areas where further study is needed for a more comparative understanding of learning. Finally, using what is known of learning in honeybees and the well-studied honeybee brain, we present a model of how various complex forms of learning may be accounted for with the same neural circuitry required for so-called simple learning types. At the neurobiological level, different learning phenomena are unlikely to be independent, and without considering this it is very difficult to correctly interpret the phylogenetic distribution of learning and cognitive abilities. WIREs Cogn Sci 2013, 4:561-582. doi: 10.1002/wcs.1248 For further resources related to this article, please visit the WIREs website.

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Contextual Pavlovian conditioning in the crab Chasmagnathus

Cited by 22 publications

References 44 publications

Protein degradation by ubiquitin–proteasome system in formation and labilization of contextual conditioning memory

Protein degradation by ubiquitin–proteasome system in formation and labilization of contextual conditioning memory

A crabs' high‐order brain center resolved as a mushroom body‐like structure

Invertebrate learning and cognition: relating phenomena to neural substrate

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