“…Currently, the demand for mushrooms in Ethiopia particularly Tigray is increasing for addressing the region's food security challenges. Various agricultural waste products such as wheat straw, grass, rice straw, barley straw, and bean straw have been used for mushroom cultivation in different parts of the world including Ethiopia [15]; [7]. In Tigray, Ethiopia, these substrates are used for animal feed and are not accessible by mushroom growers at reasonable prices.…”